In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues

Abstract

Quercetagetin, the major flavonoid in marigold (Tagetes erecta L.) inflorescence residues was extracted and purified. The content of quercetagetin after the purification was 89.91 ± 0.26 %. The in vitro antioxidant activity of quercetagetin and its potential in controlling diabetes mellitus and obesity were investigated and compared to quercetin and rutin. The 50 % inhibitory concentration (IC 50 ) values of quercetagetin on scavenging 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and hydroxyl radicals were 27.12 ± 1.31 μmol/L, 12.16 ± 0.56 μmol/L and 1833.97 ± 6.66 μmol/L, respectively. The IC 50 values of quercetagetin on α-glucosidase, α-amylase and pancreatic lipase were 180.11 ± 3.68 μmol/L, 137.71 ± 3.55 μmol/L and 2327.58 ± 12.37 μmol/L, respectively. These results indicated that quercetagetin exhibited strong in vitro antioxidant, anti-diabetic and antilipemic activities. Lineweaver-Burk plots analysis elucidated that quercetagetin inhibited α-glucosidase and α-amylase non-competitively, while its inhibition against pancreatic lipase was involved in a mixed-type pattern. Moreover, strong correlations were found between ABTS ·+ /DPPH · scavenging activities and lipase inhibitory activity (R 2 > 0.90), as well as ·OH scavenging activity and α-amylase inhibitory activity (R 2 = 0.8967).

Production of flavonoid and lignan aglycones from flaxseed and soy extracts by Bifidobacterium strains

“…Herbacetin has been described to have anti‐hyperglycaemic and anti‐hyperlipidemic properties (Veeramani et al , ). Quercetagetin exhibited strong in vitro antioxidant, anti‐diabetic and antilipidemic activities, having potential application for the treatment of obesity and diabetes (Wang et al , ).…”

Production of high antioxidant activity flavonoid monoglucosides from citrus flavanone with immobilised α‐L‐rhamnosidase in one step

“…Literature reported that aglycones have better antioxidant and anti‐inflammatory effects than diglycosides (Yang et al ., ). It was also reported that quercetin exhibits greater antioxidant activity than rutin, and therefore, it has potential benefits to treat diabetes and obesity (Wang et al ., ). The production of antioxidants by removing rhamnose from rutin was reported in literature (Cruz‐Zúñiga et al ., ).…”

Antidiabetic and antioxidant effects of Croton lobatus l. in alloxan-induced diabetic rats

“…However, pancreatic islet cells of diabetic rats treated with C. lobatus leaf methanol extracts appear normal. This suggests an inhibition of the toxic mechanisms involved in the alloxan-induced, oxidative stress-mediated destruction of pancreatic Islet cells by the antioxidant rich, flavonoid-containing C. lobatus extract inducing increased antioxidant enzyme activity, and consequently preserving Islet cells integrity [ 51 ].…”