In vitro antioxidant, antibacterial, in vivo immunomodulatory, antitumor and hematological potential of exopolysaccharide produced by wild type and mutant Lactobacillus delbureckii subsp. bulgaricus

Abstract

Biological evaluation of exopolysaccharides (EPS) produced by wild type and mutant Lactobacillus delbureckii (EPSWLD and EPSMLD) was investigated. Varying degrees of functional groups associated with polysaccharides were present thus confirming the EPS. The EPSs had strong antioxidant potential in a dose dependent (0.5-10 mg/ mL) manner. EPSWLD and EPSMLD exhibited the highest 1,1-diphemy 1-2-picryl-hydrazyl (DPPH) activity (73.4 % and 65.6 %), total antioxidant activity (1.80 % and 1.42 %), H 2 O 2 scavenging activity (88.5 % and 78.6 %) and Ferric Reducing Antioxidant Power (FRAP) (1.89 % and1.81 %) at 10 mg/mL respectively. WLD and MLD were highly susceptible to chloramphenicol, cotrimoxazole, tetracycline, erythromycin and ceftazidine and resistant to cefuroxime, gentamicin and cloxacillin. The EPSs had antibacterial activity against the test pathogens. B. subtilis and S. aureus had the highest susceptibility (26.0 mm and 23.0 mm). EPSMLD modulate the highest IgG, IgA and IgM production (68-126 mg/dL and 67-98 mg/dL and 64-97 mg/dL) in the treated tumor induced mice (TTIM). EPSWLD and EPSMLD exhibited reduction capability on the CEA level (3.99-4.35 ng/L and 4.12-4.23 ng/L) of the TTIM. EPSWLD TTIM had the highest amount of RBC, WBC and PCV (5.6 Â 10 12 %, 68000% and 42%). The EPS increased the lifespan of TTIM. In conclusion EPSWLD and EPSMLD had strong biological potential with pharmacological and neutraceutical activity.

Exopolysaccharides from probiotic bacteria and their health potential

“…EPS from Lactobacillus plantarum NCU116 increased the expression of pro-apoptotic genes such as Fas, FasL and c-Jun, through toll-like receptor-2 (TLR-2) which mediates apoptosis in mouse intestinal epithelial cancer cells [108]. EPS produced by wild and mutant Lactobacillus delbrueckii has been studied to disclose their inhibitory role on tumor cell by the reduction in carcino-embryonic antigen level of tumor induced mice [109]. Silver nanoparticles synthesized using EPS from Lactobacillus brevis demonstrates various applications in the biomedical field especially as a powerful anti-proliferative agents against various human cancer cell lines [110].…”

Selection of Wild Lactic Acid Bacteria Strains as Promoters of Postbiotics in Gluten-Free Sourdoughs

“…strains yielded amounts of EPS ranging from 380 to 737.3 mg/L. In the studies by Adebayo-Tayo et al (2018) [117] and Adebayo-Tayo et al (2020) [118], the EPS production yields were compared between mutant and wild LAB strains, where the wild strain of Lactobacillus delbrueckii has been shown to be more effective (5.57 g/L). Nonetheless, in the same study [117] the EPS yields of 5.49 and 5.58 g/L with a wild and mutant strain of Weissella confusa were obtained.…”

Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods

“…Several other authors also reported the impact of culture medium composition on the level of EPS produced by LAB [ 34 , 37 , 42 , 55 , 57 ]. In this regard, a number of media specifically developed for EPS production, including skim or partially skim milk, whey based medium, semi-defined medium, chemically defined medium and basal minimum medium have also been applied [ 58 ].…”

“…bulgaricus showed a high amount of EPS production ca. (5.5 g/L) in modified skim milk with glucose as a carbon source [ 42 ]. Additionally, S. thermophilus has been found to exhibit high EPS production in milk medium in comparison to MRS or M17 broths [ 59 ].…”