In vitro antioxidant, antimicrobial and antiproliferative studies of four different extracts of Orthosiphon stamineus, Gynura procumbens and Ficus deltoidea

Abstract

BackgroundMedicinal plants are important source of drugs with pharmacological activities. Therefore, there is always rising demands to discover more therapeutic agents from various species. Orthosiphon stamineus, Gynura procumbens and Ficus deltoidea are high valued medicinal plants of Malaysia contain rich source of phenolic and flavonoid compounds. The aims of the present study were to evaluate anti-oxidant, antimicrobial and anti-proliferative effects on A549, HeGP2 and MCF7 cell lines of four different extracts of Orthosiphon stamineus, Gynura procumbens and Ficus deltoidea.MethodologyThe leaves of all selected plants were extracted with methanol, chloroform, ethyl acetate and butanol separately with simple cold maceration. Antioxidant activity of all crude extracts were quantitatively measured against DPPH and Ferric Reducing Assay. Antimicrobial evaluation was done by Microdilution and MTT assay and antipoliferative activity of all extracts of selected plant were evaluated against A549, HePG2 and MCF7 cell lines.ResultsResults showed that methanol extract exhibited highest percentage free radical scavenging activity of almost all extracts of selected plants. Antimicrobials results showed chloroform and methanol extracts of O. stamineus extract were the two most active extracts against resistant MRSA but not S. aureus. Only methanol extract of G. procumbens showed antimicrobial activity against the tested pathogens. Chloroform and methanol extracts of F. deltoidea elicited antimicrobial activity against S. aureus but not MRSA. Antiproliferative activity against three tested cell lines results showed that ethyl acetate extract of O. stamineus showed good effect whereas methanol extract of F. deltoidea and G. procumbens exhibited good antiproliferative activity.ConclusionsThe results of the present investigation demonstrated significant variations in the antioxidant, antimicrobial and antiproliferative effects of different solvent extracts. These data could be helpful in isolation of pure potent compounds with good biological activities from the extracts of plants.

The Effects of Hydrogen Peroxide on Plant Growth, Mineral Accumulation, as Well as Biological and Chemical Properties of Ficus deltoidea

“…Solvent extracts such as the polar solvent (ethanol) has a higher content compared to semi polar (chloroform) where an increase in TPC and TFC significantly increase antioxidant activity. This was supported by Kamran et al [9] where his recent study also indicated that semi polar solvent has higher antioxidant activity compared with polar solvent. Which was not surprising, as we know that flavonoids are a large group of phenolic secondary metabolites which are widespread among plants and are involved in many plant functions [49].…”

“…It is a small plant up to 3 m tall, sometimes occurring as an epiphyte and exhibits low photosynthetic rates during its vegetative growth. Much research has been done to recognize the importance of this plant highlighting its antidiabetic [5,6], antinociceptive [7], antioxidant [8], antimicrobial [9] and anti-proliferative [10] uses. Historically, each part of this plant is known to provide benefits including reduced sugar levels in the blood [5], reduced blood pressure [10], contraction of the vagina after delivery [11], delayed menopause and also to reduce the risk of cancer [11][12][13].…”

Antibacterial, Antioxidant and Phytochemical Screening of Palestinian Mallow, Malva Sylvestris L.

“…Statistically, it is estimated that there are 750,000 plant species on Earth, of which 1-10% are used as food and medicine by both humans and animals [1,2]. Medicinal plants display several pharmacological properties such as antioxidants [3], anti-diabetes [4], antibacterial [5], antiviral [6], anticancer [7], and anti-ulcer activities [8]. The medicinal value of these plants lies in a group of bioactive organic compounds (metabolites, secondary compounds) generally classified into alkaloids, saponins, flavonoids, tannins, glycosides, anthraquinones, steroids, and terpenoids and present at different quantities and qualities within the plants [9].…”

Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice

“…The impact of different solvents on extraction yields, phytochemical constituents and biological activities such as antioxidant, antimicrobial and antiproliferative effects [36] or antidiabetic properties [12] of G. procumbens has been reported. For example, flavonoids such as myricetin, quercetin, kaempferol and apigenin were more highly detected in crude ethanolic extract than ethyl acetate or other fractions [22].…”