In vitro antioxidant capacity and in vivo hepatoprotective effect of Allophylus edulis leaf extract

Abstract

Allophylus edulis is used in folk medicine primarily for liver conditions such as hepatitis, liver cancer and liver cirrhosis. In vitro hepatoprotective activity was previously demonstrated. The aim of this work was to evaluate the antioxidant capacity and hepatoprotective effect of the ethanolic extract of A. edulis in mice. This was done by first determining the acute toxicity of the extract, evaluating the general behavior, and subsequently verifying the effect on paracetamol-induced toxic hepatitis in male mice. Additionally, the phytochemical profile was performed, and the content of total phenols and its total antioxidant capacity were quantified through the 2,2, azinobis-(3-ethylbenzothiazoline)-6 sulfonic acid radical cation (ABTS) method. The extract of A. edulis leaves did not demonstrate adverse effects up to 2000 mg/kg, p.o. Anthraquinones, flavonoids, triterpenoids, and tannins were detected. A high content of total phenolic compounds (TPC) was related to a high antioxidant capacity. Regarding the results of the biological tests, A. edulis did not affect the general behavior of the mice, and all doses tested decreased glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) activity, the main liver enzyme markers of hepatocellular damage. It is concluded that A. edulis has hepatoprotective activity, which could be related to its antioxidant activity. Keywords: Allophylus edulis; hepatoprotective; acetaminophen; liver enzymes markers; antioxidant capacity

Ethanolic Extract of Allophylus edulis Leaves Attenuates Gentamicin-Induced Acute Nephritis in Mice

“…Oral administration of A. edulis extract was previously reported to be safe and to have hepatoprotective activity, both in vitro and in vivo. In addition, a great antioxidant activity was reported in agreement with a high total phenolic content [27,14]. Moreover, quercetin, and many other phenolic compounds have been previously reported in this plant [27,28].…”

“…ex Niederl., known as "Kokũ" (Sapindaceae) leaves were collected from J.A. Saldívar, Central, Paraguay (25º26´50,4¨S y 57º 27´07,1W), identified, filed a voucher specimen in FCQ Herbarium (G Delmás 284), and extracted as described previously [14]. Briefly, the powder was extracted with previously distilled ethanol by sonicating the mixture and subsequently submitted to reflux.…”

“…Allophylus edulis, locally named "Kokũ", is a medicinal plant used as refresher, digestant, and, to a lesser extent, in the treatment of hepatitis and kidney disorders [13]. Previously, we have demonstrated that the ethanolic extract of A. edulis was able to protect the liver from acute damage induced by paracetamol in mice [14]. Nowadays, scientific information on its effect on kidney diseases is scarce.…”