In vitro Antioxidant Potential of Methanolic Extract of Symplocos racemosa Roxb.

Abstract

Many hazardous diseases are caused by free radicals accumulation. A number of investigations are focusing to find out the plant oriented natural antioxidant moieties. The basic aim of this research was to investigate the antioxidant potential of different plant parts of the crude methanolic extract of Symplocos racemosa. Symplocos racemosa (Symplocaceae) commonly known as "Lodhra" and is useful in diarrhoea, dysentery, eye diseases, fever, ulcer, scorpion sting, diabetes and liver disorders. Here the bark, leaf and root were screened for in vitro antioxidant activity study using DPPH, nitric oxide, hydroxyl radical and ABTS assay methods. Both ascorbic acid and rutin are used as a standard. The results of the study indicated that the methanolic extract of Symplocos racemosa showed potent antioxidant activity in ABTS assay method than other methods. These research investigations revealed that Symplocos racemosa is a potent source of natural antioxidants. Hence the plant can be used for management of different stress and anxiety related ailments. Keywords: Symplocos racemosa, Methanolic extract, Antioxidant activity.Asian Journal of Chemistry; Vol. 29, No. 10 (2017), 2271-2274 anticancer [17] and has beneficial effects in gynaecological disorders [18]. As there is no published data for the antioxidant potential of different parts of Symplocos racemosa, so this study was designed to investigate the antioxidant potential of the plant. EXPERIMENTAL Extraction of plant:The plant Symplocos racemosa (Family: Symplocaceae) was collected from Patbil, Karanjia, Mayurbhanj, India. Further, it was authenticated by the taxonomist Dr. Pratap Chandra Panda, Senior Scientist, Regional Plant Resources Centre (RPRC), Bhubaneswar, India and its voucher specimen (Voucher No. 10122) was deposited in the Herbarium of RPRC for further reference. After due authentication the barks, leaves and roots were dried in shade and powdered to obtain coarse powder. The coarse powder material (250 g) was subjected to extraction with methanol (95 % v/v) by using Soxhlet apparatus. The methanol extract was concentrated in vacuo and kept in a vacuum desiccator for complete removal of solvent. The yield was 9.3 % w/w with respect to dried powder [19].2,2-Diphenyl-1-picrylhydrazyl (DPPH) [Sigma-Aldrich, India], methanol, dimethyl sulphoxide, sodium nitroprusside, naphthyl ethylene diamine dihydrochloride, sulphanilic acid, potassium persulphate, ferric chloride, hydrogen peroxide, thiobarbituric acid, trichloro acetic acid, ascorbic acid and rutin were purchased from Merck India Ltd.

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