In vitro Antioxidant Studies of Fruits of Artemisia nilagirica (Clarke) Pamp.

Abstract

Antioxidant potential of fruits of Artemisia nilagirica was studied using different in vitro models like 1,1-diphenyl-2-picryl hydrazyl, 2,2-azinobis-(3-ethylbenzothizoline-6-sulphonate), nitric oxide, superoxide, hydroxyl radical and lipid peroxidation. Both the ethanol and aqueous extracts of A. nilagirica fruits at 500 μg/ml showed maximum scavenging activity (89.33% and 89.14%) in quenching 1,1-diphenyl-2-picryl hydrazyl radical. The ethanol extract showed better scavenging activity (69.78%) of 1,1-diphenyl-2-picryl hydrazyl radical followed by the scavenging of nitric oxide radical (73.25%) compared to aqueous extract. In contrast, hydroxyl and superoxide radicals were effectively scavenged by aqueous extract. Total antioxidant capacity of ethanol and aqueous extracts at 500 μg/ml concentration was found to be 56.21 and 62.78 mg ascorbic acid equivalents, respectively. However, both the extracts showed only moderate lipid peroxidation inhibition activity. They were also found to contain considerable total phenols and flavonoids suggesting their role as an effective free radical scavenger. These findings suggest that phenolics and flavonoids in the fruits provide substantial antioxidant activity.

Chlorophytum borivilianum (Safed Musli) root extract prevents impairment in characteristics and elevation of oxidative stress in sperm of streptozotocin-induced adult male diabetic Wistar rats

“…The ability of the extract to scavenge nitric oxide was determined using a method by Dastmalchi et al, [ 36 ]. Finally, reducing power scavenging activity of C. borivilianum root extract was determined according to the method by Suseela et al, [ 37 ].…”

Antioxidant activity and mechanism of commercial Rama Forte persimmon fruits (Diospyros kaki)

“…In general, the antioxidant capacity of persimmon extracts seems to be lower in a more lipophilic environment. Similar differences of antioxidant capacities between the two assays were observed in Artemisia nilagirica fruit, which presented 50% protection at 0.23 mg/mL and 0.47 mg/mL against 2-DR oxidation and lipid peroxidation, respectively ( Suseela, Gopalakrishnan & Varghese, 2010 ).…”

‘Synergistic-Cidal’ Effect of Amoxicillin Conjugated Silver Nanoparticles Against Escherichia coli

“…Total antioxidant activity of functionalized AgNPs was expressed as the number of equivalents of ascorbic acid. The phosphomolybdenum method was based on the reduction from Mo (VI) to Mo (V) by the antioxidant compound and the formation of green phosphate/ Mo (V) complex with maximum absorption observed at 695 nm [37]. The present study revealed that the antioxidant activity of AgNPs was in the increasing trend with increasing the concentration of ascorbic acid (control) and AgNPs.…”