In-vitro antioxidative potential of methanolic aerial extracts from three ethnomedicinal plants of Assam: A Comparative Study

Abstract

Antioxidants are the substances that neutralize free radicals or their actions. The traditional antioxidant phytochemicals from plants have been reported to inhibit the propagation of free radical reactions and to protect the human body from disease. The use of synthetic antioxidants has always been questioned because of their toxicity therefore researches are being carried out on plants nowadays to bring out the potent natural antioxidants. With this intent three ethnomedicinal plants of Assam viz., Calamus leptospadix Griff., Heliotropium indicum Linn., Lasia spinosa Lour. were explored with a view to evaluate their in-vitro antioxidative potentials. The methanolic aerial extracts were studied for phytochemical analysis and antioxidative properties by different invitro assays: DPPH radical scavenging method, Nitric oxide radical scavenging activity, Hydrogen peroxide radical scavenging activity, Total phenolic content and Total flavonoid content. The percentage of inhibition was calculated by comparing the absorbance values of the control and test samples. The study conducted revealed that methanolic extract of Calamus leptospadix (CL) exhibited the highest DPPH radical scavenging activity (IC50 value of 29 ± 3.24 μg/ml), Nitric oxide radical scavenging activity (233.33 ± 3.54 μg/ml), Total phenolic content (8.02 ± 0.32 μg/ml expressed as gallic acid equivalents) and Total flavonoid contents (61.71 ± 1.16 μg/ml expressed as quercetin equivalents). Methanolic extract of Lasia spinosa (LS) showed highest hydrogen peroxide radical scavenging activity (IC50 value of 221.89 ± 2.14 μg/ml). The results obtained in the present study indicate that aerial parts of Calamus leptospadix have potent, Lasia spinosa have moderate and Heliotropium indicum (HI) have mild antioxidant and free radical scavenging activity.

Phenolic Profile, Essential Oil Composition and Bioactivity of Lasia spinosa (L.) Thwaites

“…DPPH• radical scavenging abilities were increased with the increased concentration of the test samples. The MEE exhibited 81% scavenging activity at the concentration of 1 mg/mL and our result is consistent with that of Mahato et al, [27],who investigated the methanol extract of L. spinosa for free radical scavenging activities and found moderate antioxidant activity. Phenolic compounds are reported as strong antioxidant compounds in different studies [28].…”

Antibacterial Activity of Extracts of Pinus Wallachiana, Pinus Roxburgii and Cedrus Deodara Resins Against Some Common Pathogenic Bacteria

“…Our results of ethanolic extract were accordance to the research conducted by Mohanty and, Sahoo G. in which they reported that Cedrus deodara Pinus roxburgii and Pinus wallachiana showed highest zone of inhibition against S. aureus, P. aeruginosa and E. coli [41].…”

Chemo-type of essential oil of Ocimum basilicum L. from DR Congo and relative in vitro antioxidant potential to the polarity of crude extracts

“…Nowadays antioxidant phytochemicals, notably flavonoids and other polyphenol compounds from plants have been reported to inhibit the propagation of oxidant compounds like free radical and to protect the living system from disease caused by the oxidation process [8][9][10][11][12]. As a result extraction, characterization and the use of natural antioxidants from plants have been known to be a great progress [10][11][12].…”