In vitro antiplasmodial and cytotoxicity activities of crude extracts and major compounds from Goniothalamus lanceolatus

Kaharudin, Fatin Amelina; Zohdi, Rozaini Mohd; Mukhtar, Shahida Muhamad; Sidek, Hasidah Mohd; Bihud, Nur Vicky; Rasol, Nurulfazlina Edayah; Ahmad, Fauziah; Ismail, Nor Hadiani

doi:10.1016/j.jep.2020.112657

Cited by 11 publications

(11 citation statements)

References 20 publications

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“…The extract activity as antiplasmodial obtained an IC50 value of 38.61±19.06µg/mL. This shows that the extract exhibited moderate antiplasmodial activity with IC50 below 50µg/mL as reported by Kaharudin et al (2020). The compound's content of the extract is believed to be active antiplasmodial compounds.…”

Section: In Vitro Antiplasmodial Assaysupporting

confidence: 57%

Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22

et al. 2020

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Low molecular weight (LMW) antiplasmodial compounds isolated from bacteria, particularly Streptomyces have not been widely reported. This study aimed to identify LMW compounds from Streptomyces sp. GMR22 as antiplasmodial. Isolation of the LMW compounds from the supernatant of fermentation culture using solvent of n-hexane:ethylacetate (EtOAc) (85:15v/v)and identified using gas chromatography-mass spectrometry (GC-MS). Antiplasmodial assay of n-hexane:EtOAc extract was carried out in vitro against P. falciparum (3D7). Parasitemia percentage obtained through microscopic observations and 50% inhibitory concentration (IC50) obtained through probit analysis. The antiplasmodial confirmation test was done by flow cytometry using SYBR Green I for Plasmodium DNA and anti-human CD235a for erythrocytes. The LMW compounds were investigated using SwissADME for drug-likeness. n-Hexane:EtOAc extract contained 21 LMW compounds from alcohol, hydrocarbon, ester, aromatic/diester, diester, fatty acid, and triester classes, which possessed moderate antiplasmodial activity with an IC50 value of 38.61 ± 19.06 µg/mL. Confirmation by flow cytometry analysis showed that the extract at 50 µg/mL exhibited antiplasmodial activity based on a decreased Plasmodium DNA intensity as compared to the control group. The result of drug-likeness screening obtained that 3 LMW compounds were drug-likeness, namely phenylethyl alcohol, ethyl citrate, and di-n-butyl phthalate. Streptomyces sp. GMR22 produced LMW compounds as antiplasmodial, and further study was needed for identification of antiplasmodial active compounds.

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Section: In Vitro Antiplasmodial Assaysupporting

confidence: 57%

Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22

et al. 2020

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“…P. falciparum and P. vivax, among Plasmodium species, are the most virulent agents of human malaria, causing substantial global morbidity and mortality. 1 Owing to the rising number of cases of resistance to the majority of currently available medications, controlling malaria parasites is becoming increasingly difficult. Despite decades of intensive research and development efforts, there is currently no commercially available malaria vaccine.…”

Section: Introductionmentioning

confidence: 99%

“…2 As a result, chemotherapeutic agents are still in high demand for the complete treatment of malaria, and the rising rate of resistance means that finding new antimalarial drugs is a top priority. 1 Aside from clinically approved medications, various plants have long been used to prevent, treat, and/or relieve the symptoms of malaria. Medicinal plants were used by more than 80% of Africans, including Ethiopians, to meet their basic healthcare needs.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Antiplasmodial Activity of Hydroalcoholic Crude Extract and Solvent Fractions of Zehneria scabra Roots Against Plasmodium berghei in Swiss Albino Mice

Nureye¹,

Tekalign²,

Fisseha³

et al. 2021

IDR

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Background: Since drug resistance makes controlling malaria parasites a major challenge, these pioneering researchers explore and discover new novel drugs from a variety of sources. As a result, this study aimed to assess the anti-plasmodial activity of hydroalcoholic crude extract and solvent fractions of Zehneria scabra roots in mice infected with Plasmodium berghei. Methods: The antimalarial activity and safety profile of Zehneria scabra extracts were tested in a mouse model using four-day suppressive, prophylactic, and rane's tests against chloroquine-sensitive Plasmodium berghei. Mice were divided into five groups at random: group I received distilled water (10 mL/kg), group II, III, and IV received 200, 400, and 600 mg/kg of the extract, respectively, and group V received chloroquine (25 mg/kg). The antimalarial activity of the extract was determined using parasitemia levels, survival time, rectal temperature, and weight variation. Results: At all dose levels, the crude extract and solvent fractions of Zehneria scabra showed significant (p<0.05 to p<0.001) chemosuppression, with the crude extract and butanol fraction showing the highest chemosuppression (73.09% and 74.09%, respectively). Apart from suppressing parasitemia, the extract also increased survival time and secured packed cell volume reduction substantially (p<0.05 to p<0.001), while the crude extract had no significant impact on body weight or rectal temperature reduction in four-day suppressive and prophylactic models. Conclusion:The result designated that Zehneria scabra is endowed with significant antimalarial activity. These results thus support the traditional use of Zehneria scabra, for the treatment of malaria.

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“…Thus, one of the advantages of plant extracts is their complex composition. Their components have several biological activities that result in a greater total activity and this can occur due to synergy, cumulative effects, addictive properties of the constituents or bioavailability (CARMONA;PEREIRA, 2013;KAHARUDIN et al, 2020).…”

Section: Ravnmentioning

confidence: 99%

Study of encapsulation and anticancer properties of plant extracts with probiotics

Holkem¹

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In vitro antiplasmodial and cytotoxicity activities of crude extracts and major compounds from Goniothalamus lanceolatus

Cited by 11 publications

References 20 publications

Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22

Antiplasmodial Activity of The Low Molecular Weight Compounds from Streptomyces sp. GMR22

Evaluation of Antiplasmodial Activity of Hydroalcoholic Crude Extract and Solvent Fractions of Zehneria scabra Roots Against Plasmodium berghei in Swiss Albino Mice

Study of encapsulation and anticancer properties of plant extracts with probiotics

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