In vitro Antiplasmodial and Toxicity Activities of Crude Extracts and Compounds from Euclea latideus (Ebenaceae)

Abstract

Aims:In our search for new antiplasmodial agents, in vitro antiplasmodial activities of the crude extracts and isolated pure compounds were determined. In addition to the in vitro assays, in vivo acute toxicity of the crude extracts was investigated to assess the safety of the plants. Furthermore, structure elucidation of the pure compounds was also carried out to determine the identity of the isolated compounds. Study Design: Extraction of the root crude extracts of Euclea latideus was done using four solvents: hexane, dichloromethane, ethyl acetate and methanol. Isolation and purification were Original Research Article

Ethnobotanical uses, phytochemistry and biological activity of the genus Euclea: A review

“…Euclea natalensis is widely found along the eastern coast of southern Africa (Johanna, 2007). Euclea latideus is well presented in the lowlands of the tropical and to a lesser extent, in subtropical regions of the world ( Philip et al, 2018 ). A versatile medicinal plant in Ethiopia from this genus is Euclea divinorum.…”

“…Aqueous, dichloromethane, and methanol leaf and twig extracts of E. undulata have shown antimalarial activity against Plasmodium falciparum using the parasite lactate dehydrogenase assay ( Maroyi, 2017 ). E. latideus is also effective against P. falciparum especially for the chloroquine resistant strain of P. falciparum due to the presence of lupeol, betulin, and 3β-(5-hydroxy feruloyl) lup-20 (30)-ene ( Philip et al, 2018 ). The dichloromethane and methanol (1:1) root and leaf extracts of E. natalensis demonstrated promising activity in a research by Clarkson et al (2004) employing the parasite lactate dehydrogenase assay, with (IC 50 ) values of 5.1 and 5.3 mg/mL, respectively, against P. falciparum .…”

“…The dichloromethane and methanol (1:1) root and leaf extracts of E. natalensis demonstrated promising activity in a research by Clarkson et al (2004) employing the parasite lactate dehydrogenase assay, with (IC 50 ) values of 5.1 and 5.3 mg/mL, respectively, against P. falciparum . A study done by Philip et al (2018) indicated that the extracts and isolated compounds from E. latideus demonstrated antiplasmodial activity against chloroquine sensitive and chloroquine resistant strains of P. falciparum. The leaves of E. natalensis also showed antiplasmodial activity with an IC 50 of 25.6 μg/mL ( Tajuddeen et al, 2022).…”