In Vitro Antirheumatoid Arthritic Activity of Aqueous Root Extract of Clitoria Ternatea

Bharathee, Ranaweera Chathuranga; Ranjith, Pathirana; Chandana, A. K.; Jayakody, Jayakody Ralalage Anusha Chandra; Daya, Ratnasooriya Wanigasekara

doi:10.7897/2230-8407.0512188

Cited by 9 publications

(6 citation statements)

References 5 publications

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“…Because in arthritic conditions autoantigens are produced due to protein denaturation, thus NSAIDs used in such conditions impair heat-induced denaturation of proteins. Therefore, the outcomes of this study confirmed the anti-arthritic potential of NA-2 formerly reported by Perveen et al (2014) (14).…”

Section: Analgesic Activitysupporting

confidence: 91%

Evaluation of Anti-inflammatory, Antioxidant and Xanthine Oxidase Inhibitory Potential of N-(2-hydroxy phenyl) Acetamide

Arif¹,

Majeed

Simjee³

et al. 2022

Pak Euro. J. Med & LS

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Nonsteroidal anti-inflammatory drugs has long been used to treat various pathological conditions like arthritis, gout, diabetes, cancer, and cardiovascular diseases. Although exploring the effective anti-inflammatory agents possessing antioxidant along with pain relieving properties is still challenging. Acetamide-containing compounds are known to possess analgesic and anti-inflammatory activity. The purpose of this study is to evaluate the anti-inflammatory, anti-oxidant, xanthine oxidase, and analgesic activity of an acetamide derivative, N-(2-Hydroxyphenyl) Acetamide. In-vitro anti-inflammatory and antioxidant activities were assessed by protein denaturation inhibition and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, respectively. Xanthine oxidase potential was also evaluated. In-vivo anti-inflammatory activity was determined by the Formalin-induced paw edema method and analgesic potential was evaluated by an acetic acid-induced writhing test. Results of in-vitro anti-inflammatory activity showed significant (p< 0.001) inhibition of protein denaturation by N-(2-Hydroxyphenyl) Acetamide at both100 and 300 µg/ml. It also exhibited high radical scavenging activity (88.16%) by DPPH at 300 µg/ml. In-vivo Formalin-induced paw edema method revealed significant (p < 0.05) inhibition of paw edema when compared with control and formalin groups. Likewise, NA-2 also significantly (p < 0.001) reduced the number of writhes in the Acetic acid-induced writhing test. Moreover, in both aforementioned in-vivo tests, NA-2 showed comparable results with Indomethacin (10 mg/kg). This study demonstrates that N-(2-Hydroxyphenyl) Acetamide possesses substantial anti-inflammatory, antioxidant, and analgesic activity.

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Section: Analgesic Activitysupporting

confidence: 91%

Evaluation of Anti-inflammatory, Antioxidant and Xanthine Oxidase Inhibitory Potential of N-(2-hydroxy phenyl) Acetamide

Arif¹,

Majeed

Simjee³

et al. 2022

Pak Euro. J. Med & LS

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“…According to Ranaweera et al [15], a well-known in vitro bioassay model to assess the anti-rheumatoid arthritic potential of an aqueous root extracted from the C. ternatea, through the inhibition of heat denaturation of the albumin protein, claimed to act as an index of anti-arthritic activity. In this study, the root extract of the C. ternatea plant was checked against a fresh hen's egg albumin, in which the results stated that the extract inhibited the denaturation of protein, which is one of the leading causes of rheumatoid arthritis.…”

Section: Anti-arthritic and Anti-inflammatory Potentialmentioning

confidence: 99%

The Potential for the Implementation of Pea Flower (Clitoria ternatea) Health Properties in Food Matrix

2023

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Clitoria ternatea is a revered flower and plant in botanical science. While its health benefits are only recently gaining popularity, the plant itself has been the recipient of many traditional and indigenous medicines, including that of Ayurvedic medicine in South Asia. The peculiar property of this flower is its ability to change color depending on its pH. This review article encompasses the literature surrounding this plant and its valuable flower and attempts to cover all aspects of its benefits in the food matrix, including its existing applications. It also aims to look at the flower from a holistic perspective and imagine it as a source of future food.

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“…Three flavonol glycosides, kaempferol-3-β-glucoside, quecetin-3-β-glucoside, and myricetin-3-β-glucoside were isolated from the petals of C. ternatea, amongst these particularly quercetin-3β-D-glucosides were identified by NMR studies, and LC/MS studies in highest quantities particularly in methanolic extracts of the C. ternatea flower petals 15 . The hydroalcoholic extracts of blue flower petal possess potential antioxidant and anti-inflammatory activities, and its disease modifying activities has been correlated to its flavonoid components 16,17,18 .…”

Section: Scheme 1: Role Of Tnfα / Tnfr1 Mediated Signaling In Inflammmentioning

confidence: 99%

Untitled

2018

IJPSR

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TNF-α plays pivotal role in initiation of inflammation via its interaction with TNFR1 in an O 2 .induced oxidative stress dependent manner. The present study investigated whether the anti-oxidant effect of hydroalcoholic extract of Clitoria ternatea (CTE), could facilitate the anti-inflammatory effect of TNFR1 neutralization in context to carrageenan-induced acute inflammation in mice. Sub-plantar injection of λ-carrageenan (0.1% in saline) elicited profound inflammatory reactions in plantar tissue 0-12 hour (h) post carrageenan injection (p.c.i.), as evident from increased PMN infiltration (myeloperoxidase activity), local and systemic release of cytokines and chemokines, free radical generation and oxidative stress in male Swiss albino mice (20-22g, 3-4 weeks of age). The inflammatory reactions were attenuated from 6-12 h pci in CTE (50 mg/kg) and QG (2.5 mg/kg) pre-treated mice, and were potentiated followed by TNFR1 neutralization with anti-TNFR1 antibody (10 μg/mouse) in terms of significant decreases (p < 0.05) in PMN infiltration, pro-inflammatory cytokines and chemokines as well as ROS/RNS generation and oxidative stress in parallel to decreased TNFR1 and NF-κB mediated COX-2 and iNOS expression from 4-12 h pci. Results suggested that anti-inflammatory effect exerted by CTE or QG were potentiated following antibody mediated TNFR1 neutralization which might become a reliable combinatorial approach to combat inflammatory diseases in future therapeutic research.

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In Vitro Antirheumatoid Arthritic Activity of Aqueous Root Extract of Clitoria Ternatea

Cited by 9 publications

References 5 publications

Evaluation of Anti-inflammatory, Antioxidant and Xanthine Oxidase Inhibitory Potential of N-(2-hydroxy phenyl) Acetamide

Evaluation of Anti-inflammatory, Antioxidant and Xanthine Oxidase Inhibitory Potential of N-(2-hydroxy phenyl) Acetamide

The Potential for the Implementation of Pea Flower (Clitoria ternatea) Health Properties in Food Matrix

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