In vitro Antisalmonellal and Antioxidant Properties of Mangifera indica L. Stem Bark Crude Extracts and Fractions

Noghogne, Landric; Gatsing, Donatien; Fotso,; Kodjio, Norbert; Sokoudjou, Jean Baptiste; Kuiaté, Jules Roger

doi:10.9734/bjpr/2015/13390

Cited by 14 publications

(9 citation statements)

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“…The antioxidant activity of the extracts could be explained by their richness in polyphenolic substances, tannins and more particularly in total flavonoids. These results are in agreement with those of several authors, who reported a positive correlation between all phenolic content and antioxidant activity [31,5]. Hydroethanolic and aqueous extracts of B. rufescens leaves and stem barks showed IC 50 values between 9.780 and 19.531 μg/ml.…”

Section: Antioxidantsupporting

confidence: 93%

In vitro Antisalmonella and Antioxidant Activity of Hydroethanolic and Aqueous Extracts of Bauhinia rufescens Leaf and Stem Bark Extracts

Djenguemtar

Konack

Sokoudjou

et al. 2022

MRJI

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In Chad, enteric fever remains a major public health problem where it is still endemic due to the precariousness of life hygiene combined with the abusive and inappropriate use of antibiotics. Objective: The aim of this work was to evaluate the in vitro antisalmonella and antioxidant activity of extracts from the leaves and stem bark of B. rufescens. Methods: Phytochemical screening of these extracts was performed by standard methods to justify the observed activities. The antisalmonella activity was evaluated using the liquid microdilution method. Antioxidant activity of these extracts was determined by investigating their 1, 1-diphenyl-2 picrylhydrazyl (DPPH●) antiradical and iron reducing capacities. Results: The Minimum Inhibitory Concentrations (MICs) were varied from 256 to 1024 µg/ml. The 95% hydroethanolic extract of the leaves exhibited higher DPPH● antiradical activity than all extracts and IC50s ≤ 20 μg/ml for all extracts tested. Conclusion: These results showed that the 95% hydroethanolic extract of B. rufescens leaves possess in vitro antisalmonella and antioxidant activities and could be used for in vivo antisalmonella and antioxidant studies.

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Section: Antioxidantsupporting

confidence: 93%

In vitro Antisalmonella and Antioxidant Activity of Hydroethanolic and Aqueous Extracts of Bauhinia rufescens Leaf and Stem Bark Extracts

Djenguemtar

Konack

Sokoudjou

et al. 2022

MRJI

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“…It is in this perspective that the hydro-ethanolic extract of Canarium schweinfurthii has been explored for its antisalmonellal activity and its bioactive compounds. Several plants are traditionally used against human salmonellosis [41][42][43][44][45][46] and avian salmonellosis [24][25][26]47]. Plants with high anti-salmonellal potential that show promise for the control of avian salmonellosis include Aloe secundiflora [47], Thymus vulgaris [48], Curcuma longa and Scutellaria baicalensis [25] and Erica mannii [27].…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization and in vitro anti-salmonellal activity of compounds from stem bark extract of Canarium schweinfurthii

Sokoudjou

Atolanı

Njateng

et al. 2020

BMC Complement Med Ther

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Background Bacteria belonging to the Salmonella genus are major concern for health, as they are widely reported in many cases of food poisoning. The use of antibiotics remains a main stream control strategy for avian salmonellosis as well as typhoid and paratyphoid fevers in humans. Due to the growing awareness about drug resistance and toxicities, the use of antibiotics is being discouraged in many countries whilst advocating potent benign alternatives such as phyto-based medicine. The objective of this work was to isolate, characterise the bioactive compounds of Canarium schweinfurthii; and evaluate their anti-salmonellal activity. Methods The hydro-ethanolic extract of Canarium schweinfurthii was fractionated and tested for their anti-salmonellal activity. The most active fractions (i.e. chloroform and ethyl acetate partition fractions) were then explored for their phytochemical constituents. Fractionation on normal phase silica gel column chromatography and size exclusion chromatography on Sephadex LH-20 led to the isolation of four compounds (maniladiol, scopoletin, ethyl gallate and gallic acid) reported for the first time in Canarium schweinfurthii. Results Result indicated that scopoletin and gallic acid had greater activity than the crude extracts and partition fractions. Among the isolated compounds, scopoletin showed the highest inhibitory activity with a MIC of 16 μg/ml against Salmonella Typhimurium and Salmonella Enteritidis. Conclusions The overall results of this study indicates that the hydro-ethanolic extract as well as some of isolated compounds have interesting anti-salmonellal activities that could be further explored for the development of potent therapy for salmonellosis. Furthermore, the study adds credence to the folkloric applications of the plant.

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“…The free radical scavenging activities of Dracaena deisteliana leaves extracts were evaluated using the DPPH assay method as described by [21] and [22]. Briefly, the extract (2000 μg/ml) was twofold serially diluted with methanoln, hence 100 μl of diluted extract were mixed with 900 μl of 0.3 mM 2,2-diphenyl-1-picrylhydrazyl (DPPH) methanol solution to give a final extract concentration of 12.5, 25, 50, 100 and 200 μg/ml.…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo Antioxidant potential of aqueous and hydroethanolic extracts leaves ofDracaena deistelianaEng. (Dracacenaceae)

Feudjio

Goka

Josias

et al. 2023

Preprint

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Enterobacteria such as those of the genus Salmonella express the oxyR gene that codes for several proteins that allow it to resist free radicals. This resistance of Salmonella is often at the origin of an overproduction of these free radicals that can lead to oxidative stress. To investigate the antioxidant activity in vitro (aqueous and hydroethanolic extracts in vivo (of the 55% hydroethanolic extract) of Dracaena deisteliana leaves in albino rats of Wistar strain previously infected with the Salmonella strain Typhi ATCC 6539. The in vitro antioxidant activity of these extracts was determined by studying their anti-radical power with DPPH•, then their iron-reducing power and determining their flavonoids and total phenols content. In vivo antioxidant activity was determined in plasma and tissues of albino rats of wistar strain aged between 8 and 10 weeks previously infected with Salmonella typhi ATCC 6539. These infected rats concurrently received daily doses of Dracaena deisteliana extract (10, 20 and 51.84 mg/kg) or ciprofloxacin (14 mg/kg) as positive control, for 15 days. At the end of the treatment period, the animals were sacrificed and blood, liver, kidney, heart, lung, spleen testis and ovaris were collected for evaluation of antioxidants parameters, which included malondialdehyde, nitric oxide, catalase and peroxidase, as well as biological responses. Regarding in vitro antioxidant tests, the 65% hydroethanolic extract showed an anti-radical activity with DPPH• superior to that of all hydroethanolic extracts at 100 μg/ml. However, the infused showed lower antiradical activity than all extracts at 12.5 and 200 μg/ml concentrations. The 55% hydroethanolic extract had the best IC50of (11.99 μg/ml). The iron reducing power of this extract was higher than the other extracts at 200 μg/ml. The highest levels of flavonoids and total phenols were obtained respectively with the 55% and 95% hydroethanolic extract. The hydroethanol extract of Dracaena deisteliana (10, 20 and 51.84 mg/kg) cured the infected rats between the 9th and 13th day of treatment. The extract also significantly reduced (p < 0.05) blood malondialdehyde and nitric oxide levels, and significantly increased (p >0.05) the activity of catalase and peroxidase in the infected rats. The results suggest that leaves extract of Dracaena deisteliana contains antisalmonella and antioxidant substances, which could be used for the treatment of typhoid fever and another salmonellosis. In addition, 55% hydroethanolic extract of this plant possesses antisalmonella activity and reduces the state of oxidative stress caused by S. typhi during rat's infection.

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In vitro Antisalmonellal and Antioxidant Properties of Mangifera indica L. Stem Bark Crude Extracts and Fractions

Cited by 14 publications

References 0 publications

In vitro Antisalmonella and Antioxidant Activity of Hydroethanolic and Aqueous Extracts of Bauhinia rufescens Leaf and Stem Bark Extracts

In vitro Antisalmonella and Antioxidant Activity of Hydroethanolic and Aqueous Extracts of Bauhinia rufescens Leaf and Stem Bark Extracts

Isolation, characterization and in vitro anti-salmonellal activity of compounds from stem bark extract of Canarium schweinfurthii

In vitro and in vivo Antioxidant potential of aqueous and hydroethanolic extracts leaves ofDracaena deistelianaEng. (Dracacenaceae)

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