In vitro antiviral activity of Thymbra spicata L. extract on bovine respiratory viruses (BCoV, BPIV-3, BRSV, BVDV and BoHV-1)

Toker, Eda Baldan; Yeşilbağ, Kadir

doi:10.1111/jam.15418

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…Treatment of these viruses with 1.5% T. spicata L. extract resulted in a reduction of infectious virions by 99.44%, 100.0%, 94.38%, 99.97% and 99.87% for BCoV, BPIV‐3, BRSV, BVDV and BoHV‐1, respectively. The extract of T. spicata L. also demonstrated strong dose‐dependent inhibition of viral replication in vitro for all the mentioned viruses 31 …”

Section: Discussionmentioning

confidence: 89%

“…The extract of T. spicata L. also demonstrated strong dose-dependent inhibition of viral replication in vitro for all the mentioned viruses. 31 Rosmarinic acid, vitexin and gallic acid were found the highest amount in leaf subextract. Rosmarinic acid is a phenolic acid consisting of an ester of caffeic acid and 3,4-dihydroxy phenyl lactic acid and is a bioactive phytochemical that can be found in many plants as ethnomedicines.…”

Section: Quantitative Analysis Of Secondary Metabolitesmentioning

confidence: 92%

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Qualitative and quantitative phytochemicals of essential oils and extracts of Thymbra spicata subsp. spicata L. as a spice for diabetes mellitus

Yuca,

Civaş,

Tekman

et al. 2023

Flavour & Fragrance J

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The aim of this paper was to evaluate the antidiabetic and antioxidant activities of methanolic extracts, n‐hexane, dichloromethane, ethyl acetate, butanol, aqueous subextracts and essential oils of flowers, roots, leaves and aerial parts of Thymbra spicata subsp. spicata, which has been utilized in the public medicine systems of Turkey, Greece, Egypt and Rome for the treatment of asthma and bronchitis, as well as for flavour and aroma in the food industry and protection. Quantitative determination of secondary metabolites in the most effective samples of the plant was also analysed by LC–MS/MS. Moreover, the chemical composition of essential oils of different parts of the plant was analysed via GC‐FID and GC/MS. The main constituents in the flower, leaf and aerial part were found to be carvacrol (75.6%), γ‐terpinene (10.5%), carvacrol (73.3%), γ‐terpinene (9.5%), p‐cymene (8.6%), carvacrol (76.1.8%) and p‐cymene (7.3%), respectively. Quinic acid, caffeic acid, vanillic acid, naringin, hesperidin and rosmarinic acid were measured in four ethyl acetate subextracts, and rosmarinic acid was found to have the highest amount in the flower subextract with a value of 48095.1083 ng/mL. The ethyl acetate extract of flowers showed the best activity with a 326 ± 12 μg/mL IC50 value, while the standard acarbose IC50 value was 4143 ± 243 μg/mL. It was determined that the leaf ethyl acetate extract, in particular, had a very high % inhibition value on ABTS·+ (99.137 ± 0.011% inhibition) and DPPH• (41.068 ± 0.031% inhibition). It is thought that the plant, which has been used as a spice in the kitchen for centuries by the public, can be safely used due to its high antidiabetic and antioxidant effects.

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Section: Discussionmentioning

confidence: 89%

Section: Quantitative Analysis Of Secondary Metabolitesmentioning

confidence: 92%

Qualitative and quantitative phytochemicals of essential oils and extracts of Thymbra spicata subsp. spicata L. as a spice for diabetes mellitus

Yuca,

Civaş,

Tekman

et al. 2023

Flavour & Fragrance J

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“…For laboratory studies, the epithelial cell line Madin Darby Bovine Kidney (MDBK) is commonly used for the in vitro multiplication of BVDV [ 106 , 107 , 108 ], BoHV-1 [ 107 , 109 ], and BoHV-4 [ 110 , 111 , 112 ]. In addition, the susceptibility of primary endometrial cultures to BoHV-4 and BVDV has been reported [ 102 , 113 , 114 ].…”

Section: Challenges and Obstacles In Bovine Embryo-maternal Communica...mentioning

confidence: 99%

Mesenchymal Stem Cells in Embryo-Maternal Communication under Healthy Conditions or Viral Infections: Lessons from a Bovine Model

Calle

Ramírez

2022

Cells

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Bovine mesenchymal stem cells are a relevant cell population found in the maternal reproductive tract that exhibits the immunomodulation capacity required to prevent embryo rejection. The phenotypic plasticity showed by both endometrial mesenchymal stem cells (eMSC) and embryonic trophoblast through mesenchymal to epithelial transition and epithelial to mesenchymal transition, respectively, is essential for embryo implantation. Embryonic trophoblast maintains active crosstalk via EVs and soluble proteins with eMSC and peripheral blood MSC (pbMSC) to ensure the retention of eMSC in case of pregnancy and induce the chemotaxis of pbMSC, critical for successful implantation. Early pregnancy-related proteins and angiogenic markers are detected as cargo in EVs and the soluble fraction of the embryonic trophectoderm secretome. The pattern of protein secretion in trophectoderm-EVs changes depending on their epithelial or mesenchymal phenotype and due to the uptake of MSC EVs. However, the changes in this EV-mediated communication between maternal and embryonic MSC populations infected by viruses that cause abortions in cattle are poorly understood. They are critical in the investigation of reproductive viral pathologies.

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“…T. spicata leaves and flowers are used as food ingredients in salad and in tea infusions. Different biological and bio-functional effects have been reported for T. spicata including anti-oxidant and antisteatotic [ 12 ], antihypercholesterolaemic [ 13 , 14 ], antitumoral [ 15 ], antiviral [ 16 ], and antimicrobial [ 17 , 18 , 19 ] activities.…”

Section: Introductionmentioning

confidence: 99%

Nutritional and Physiological Properties of Thymbra spicata: In Vitro Study Using Fecal Fermentation and Intestinal Integrity Models

Khalil,

Piccapane,

Vacca

et al. 2024

Nutrients

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(Poly)phenolic-rich Mediterranean plants such as Thymbra spicata have been associated with several health-promoting effects. The nutritional value, as well as physiological interaction of T. spicata with the gastrointestinal tract, has not been investigated before. The nutritional composition of T. spicata leaves was here characterized by standard analytical methods. T. spicata leaves were subjected to ethanolic extraction, simulated gastrointestinal digestion, and anaerobic microbial gut fermentation. Phenols/flavonoid contents and radical scavenging activity were assessed by colorimetric methods. The volatile organic compounds (VOCs) were detected by gas chromatography coupled with mass spectrometry. The effect on intestinal integrity was evaluated using a Caco-2 monolayers mounted in a Ussing chamber. T. spicata contains a high amount of fiber (12.3%) and unsaturated fatty acids (76% of total fat). A positive change in VOCs including short-chain fatty acids was observed without significant change in viable microbe. T. spicata and carvacrol (main phenolic compound) enhanced ionic currents in a concentration-dependent manner without compromising the Caco-2 monolayer’s integrity. These effects were partially lost upon simulated digestion and completely abolished after colonic fermentation in line with polyphenols and carvacrol content. Conclusion: T. spicata represents a promising nutrient for the modulation of gut microbiota and the gut barrier. Further studies must better define its mechanisms of action.

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In vitro antiviral activity of Thymbra spicata L. extract on bovine respiratory viruses (BCoV, BPIV-3, BRSV, BVDV and BoHV-1)

Cited by 6 publications

References 33 publications

Qualitative and quantitative phytochemicals of essential oils and extracts of Thymbra spicata subsp. spicata L. as a spice for diabetes mellitus

Qualitative and quantitative phytochemicals of essential oils and extracts of Thymbra spicata subsp. spicata L. as a spice for diabetes mellitus

Mesenchymal Stem Cells in Embryo-Maternal Communication under Healthy Conditions or Viral Infections: Lessons from a Bovine Model

Nutritional and Physiological Properties of Thymbra spicata: In Vitro Study Using Fecal Fermentation and Intestinal Integrity Models

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