In vitro approaches to assess the hazard of nanomaterials

Drašler, Barbara; Sayre, Phil; Steinhäuser, Klaus Günter; Petri‐Fink, Alke; Rothen‐Rutishauser, Barbara

doi:10.1016/j.impact.2017.08.002

Cited by 189 publications

(203 citation statements)

References 156 publications

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“…Monitoring of NPs and nanomaterials is a challenge and need more advancements in tracking their motion in systems. In vitro assessments have already been done and reported by many research groups that examined the effects of nanomaterials on cells in terms of toxicity and carcinogenicity (Drasler et al, 2017;Liu, 2006). The adversity of the nanomaterials lies in their novel and exotic physicochemical properties.…”

Section: Limitations and Upcoming Challengesmentioning

confidence: 99%

Nanomaterials for removal of waterborne pathogens

Ojha

2020

Waterborne Pathogens

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Section: Limitations and Upcoming Challengesmentioning

confidence: 99%

Nanomaterials for removal of waterborne pathogens

Ojha

2020

Waterborne Pathogens

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“…These studies suggest that 3D-microfluidic systems, which are getting use of both 3D microenvironment and flow properties, are valuable technologies to better understand the spatiotemporal performance of nanomaterials and better guide researchers for a rational design [46,47]. There are various in-depth review articles which discusses the importance of culture components, co-culture conditions, presence of 3D microenvironment or microfluidic systems during preclinical evaluation of drugs and biotechnological biomolecules [48][49][50][51]. Such reports points out that future studies focusing on the toxicity and genotoxicity of GBMs should also consider using physiologically relevant experimental conditions that will mimic an in vivo particle exposure scenario.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

A closer look at the genotoxicity of graphene based materials

et al. 2019

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Graphene-based materials (GBMs) have attracted many scientists because of their optical, thermal, mechanical and electronic properties. Their good dispersibility in different type of solvents including water, the possibility to formulate them according to desired function, and the wide surface area, which can allow various chemical modifications, expanded the use of these materials in biological systems. For these reasons, GBMs have been extensively studied in vitro and in vivo in the biomedical field. However, the toxicity and genotoxicity of GBMs must be thoroughly investigated before they can be translated into clinical settings. The main mechanism of graphene toxicity is thought to be caused by reactive oxygen species produced in cells, which in turn interact with various biomolecules including DNA. In this review we aimed to discuss different genotoxicity studies performed with GBMs with specific focus on the different cell types and conditions. By comparing and discussing such reports, scientists will be able to engineer non toxic GBMs for future preclinical and/or clinical studies. In order to allow a safer and faster transition to clinic, future studies should involve state-ofthe-art technologies such as systems biology approaches or three-dimensional microfluidic systems, which can better predict the normal physiological scenario.

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“…In addition to a variety of in vitro end point assessments, OMICS approaches allow for comparison of different in vitro systems as well as better correspondence to in vivo exposure scenarios (Kinaret et al, 2017b;Drasler et al, 2017). Combination of different OMICS methods can inform more thoroughly about ENMs MOA, which could be further used in ENM grouping approaches, as suggested by Riebeling et al (2017).…”

Section: Molecular Effectsmentioning

confidence: 99%

“…Combination of different OMICS methods can inform more thoroughly about ENMs MOA, which could be further used in ENM grouping approaches, as suggested by Riebeling et al (2017). Gene expression profiling of co-cultures and 3D cultures, as well as systems taking into account exposure route such as Air Liquid Interface cultures (Latvala et al, 2016;Kletting, 2017), better resemble the in vivo exposure scenarios, but before clear conclusions about the ENM MOA can be drawn, cell type-specific responses need to be addressed by omics approaches (Drasler et al, 2017;Snyder-Talkington et al, 2015). The importance of multi-omics approaches in the study of MOA of toxicants has been demonstrated in several toxicogenomics studies (Jayapal, 2012;Gavin, 2016).…”

Section: Molecular Effectsmentioning

confidence: 99%

“…However, responding to the constant pressure in developing reliable and efficient alternative screening methods, many studies tried to predict the potential ENMs effects in vivo by analyzing their effects in vitro (Drasler et al, 2017;Braakhuis, 2015;Törnqvist et al, 2014;Bachler et al, 2015). We recently demonstrated that carbon based nanomaterials have distinct transcriptional MOA between in vivo and in vitro experimental settings and that commonalities are to be found by using comprehensive gene network models (Kinaret et al, 2017b).…”

Section: Introductionmentioning

confidence: 99%

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Multi-omics analysis of ten carbon nanomaterials effects highlights cell type specific patterns of molecular regulation and adaptation

et al. 2018

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New strategies to characterize the effects of engineered nanomaterials (ENMs) based on omics technologies are emerging. However, given the intricate interplay of multiple regulatory layers, the study of a single molecular species in exposed biological systems might not allow the needed granularity to successfully identify the pathways of toxicity (PoT) and, hence, portraying adverse outcome pathways (AOPs). Moreover, the intrinsic diversity of different cell types composing the exposed organs and tissues in living organisms poses a problem when transferring in vivo experimentation into cell-based in vitro systems.To overcome these limitations, we have profiled genome-wide DNA methylation, mRNA and microRNA expression in three human cell lines representative of relevant cell types of the respiratory system, A549, BEAS-2B and THP-1, exposed to a low dose of ten carbon nanomaterials (CNMs) for 48 h. We applied advanced data integration and modelling techniques in order to build comprehensive regulatory and functional maps of the CNM effects in each cell type.We observed that different cell types respond differently to the same CNM exposure even at concentrations exerting similar phenotypic effects. Furthermore, we linked patterns of genomic and epigenomic regulation to intrinsic properties of CNM. Interestingly, DNA methylation and microRNA expression only partially explain the mechanism of action (MOA) of CNMs. Taken together, our results strongly support the implementation of approaches based on multi-omics screenings on multiple tissues/cell types, along with systems biology-based multi-variate data modelling, in order to build more accurate AOPs.

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In vitro approaches to assess the hazard of nanomaterials

Cited by 189 publications

References 156 publications

Nanomaterials for removal of waterborne pathogens

Nanomaterials for removal of waterborne pathogens

A closer look at the genotoxicity of graphene based materials

Multi-omics analysis of ten carbon nanomaterials effects highlights cell type specific patterns of molecular regulation and adaptation

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