In Vitro Assay of Ethanolic Heat Reflux Extract of<i>Nicotiana tabacum</i>L. var Virginia Against Nosocomial Bacteria Pathogen

Pramono, Andri; Fauzantoro, Ahmad; Hidayati, Irma Rizki; Hygea, Arina; Puspita, O. S.; Muktamiroh, Hikmah; Simanjuntak, Kristina; Gozan, Misri

doi:10.1088/1742-6596/970/1/012021

Cited by 11 publications

(6 citation statements)

References 3 publications

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“…The reflux process used was the maceration process at hot temperatures with a low boiling point solvent. The reflux process in absorbing the target compound using a fume hood to avoid inhalation of diethyl ether vapor by researchers as a result of the inability of the coolant to retain solvent vapor will support to break down cell walls so that the compound can be absorbed optimally [8][9][10]. Heating causes swelling and tremendous coalescence of the oil cells and other constituents.…”

Section: Introductionmentioning

confidence: 99%

Isolation of piperine from black pepper (Piper nigrum) in the provision of standard compounds for natural chemical practice and research activities

Sulman

2021

J. Pijar MIPA

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Research conducted to meet the needs of standard compounds in practical and research activities. The use of standard compounds improves the quality of a study. Piperine is the main secondary metabolite compound of the alkaloid group contained in Black PepperÃÂ Piper nigrumÃÂ L. The isolation method used is reflux and recrystallization methods. The reflux process in absorbing piperine compounds with diethyl ether solvent will help break down the cell walls to absorb the compounds optimally. Recrystallization was repeated 7-8 times until the crystals were pale yellow. The purity test used the TLC test with a solvent ratio of n-hexane and chloroform 6:4 with an Rf value of 0.04 cm. GCMS confirmation of 85% purity, UV-Visible spectroscopic test maximum wavelength 343 with absorbance 0.865 and FTIR test confirmed wave number 928 cm-1 (CO), 996 cm-1 ((CH bending of trans Ã¢â¬âCH=CH-), 1028 cm-1(=COC sym. st.), 1250 cm-1 (=COC asym. st.), 1440 cm-1 (methylenedioxy CH2bending), 1581 cm-1 (arom. C=C st.), 1631 cm-1 (-CO-N-). Based on the confirmed test results, the isolated compound is piperine from the alkaloid group. Piperine compounds can be used as standard compound preparations for testing the purity of compounds in research and chemistry lab work on natural ingredients.

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Section: Introductionmentioning

confidence: 99%

Isolation of piperine from black pepper (Piper nigrum) in the provision of standard compounds for natural chemical practice and research activities

Sulman

2021

J. Pijar MIPA

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“…The in vitro antibacterial activity of developed hydrogels was determined by Kirby-Bauer diffusion [ 25 ]. P. aeruginosa GIM1.551, S. aureus ATCC 25923, P. suis PM-29, and S. epidermidis LY8 were cultured for 12 h at 37°C in Tryptic Soy Broth (TSB; Qingdao Hope Bio-Technology Co., Ltd., China) liquid medium, the concentration of the bacterial suspension was further adjusted to 0.5 McFarland turbidity, and then 200 μL of bacterial suspension was uniformly transferred to the TSB solid medium.…”

Section: Methodsmentioning

confidence: 99%

Sustained release of alginate hydrogel containing antimicrobial peptide Chol-37(F34-R) in vitro and its effect on wound healing in murine model of Pseudomonas aeruginosa infection

Shi,

Dong,

Chen

et al. 2023

J Vet Sci

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Background Antibiotic resistance is a significant public health concern around the globe. Antimicrobial peptides exhibit broad-spectrum and efficient antibacterial activity with an added advantage of low drug resistance. The higher water content and 3D network structure of the hydrogels are beneficial for maintaining antimicrobial peptide activity and help to prevent degradation. The antimicrobial peptide released from hydrogels also hasten the local wound healing by promoting epithelial tissue regeneration and granulation tissue formation. Objective This study aimed at developing sodium alginate based hydrogel loaded with a novel antimicrobial peptide Chol-37(F34-R) and to investigate the characteristics in vitro and in vivo as an alternative antibacterial wound dressing to treat infectious wounds. Methods Hydrogels were developed and optimized by varying the concentrations of crosslinkers and subjected to various characterization tests like cross-sectional morphology, swelling index, percent water contents, water retention ratio, drug release and antibacterial activity in vitro , and Pseudomonas aeruginosa infected wound mice model in vivo . Results The results indicated that the hydrogel C proved superior in terms of cross-sectional morphology having uniformly sized interconnected pores, a good swelling index, with the capacity to retain a higher quantity of water. Furthermore, the optimized hydrogel has been found to exert a significant antimicrobial activity against bacteria and was also found to prevent bacterial infiltration into the wound site due to forming an impermeable barrier between the wound bed and external environment. The optimized hydrogel was found to significantly hasten skin regeneration in animal models when compared to other treatments in addition to strong inhibitory effect on the release of pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-α). Conclusions Our results suggest that sodium alginate -based hydrogels loaded with Chol-37(F34-R) hold the potential to be used as an alternative to conventional antibiotics in treating infectious skin wounds.

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“…The antibacterial activity of Chol-37(F34-R) was detected using the Kirby-Bauer diffusion method [ 40 ]. Four bacterial strains were used as experimental strains: L. monocytogenes CICC21634 cultured with BHI, P. aeruginosa GIM1.551 cultured with TSB, S. aureus ATCC25923, and S. typhimurium SL1344 cultured with LB medium.…”

Section: Methodsmentioning

confidence: 99%

Enhancing the antibacterial activity of antimicrobial peptide PMAP-37(F34-R) by cholesterol modification

et al. 2020

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Background The problem of increasing resistance against conventional antibiotics has drawn people’s attention. Therefore, the development of novel antibacterial agents with effective and safe therapeutic effects is imminent. Antimicrobial peptides (AMPs) are considered a promising class of antibacterial agents due to their broad antibacterial spectrum. Results In this study, on the basis of our previously studied peptide PMAP-37(F34-R), a novel antimicrobial peptide Chol-37(F34-R) was developed by N-terminal cholesterol modification to increase hydrophobicity. We observed that the N-terminal cholesterol-modified Chol-37(F34-R) showed higher antimicrobial activity than PMAP-37(F34-R) in vitro. Chol-37(F34-R) also exhibited effective anti-biofilm activity and may kill bacteria by improving the permeability of their membranes. Chol-37(F34-R) exerted high stability in different pH, salt, serum, and boiling water environments. Chol-37(F34-R) also showed no hemolytic activity and substantially low toxicity. Furthermore, Chol-37(F34-R) exhibited good potency of bacteria eradication and promoted wound healing and abscess reduction in infected mice. Meanwhile, in S. aureus ATCC25923-infected peritonitis model, Chol-37(F34-R) exhibited an impressive therapeutic effect by reducing the decrease in systemic bacterial burden and alleviating organ damage. Conclusions Our findings suggested that the N-terminal cholesterol modification of PMAP-37(F34-R) could improve antibacterial activity. Chol-37(F34-R) displayed excellent bactericidal efficacy and impressive therapeutic effect in vivo. Thus, Chol-37(F34-R) may be a candidate for antimicrobial agents against microbial infection in the clinic.

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In Vitro Assay of Ethanolic Heat Reflux Extract ofNicotiana tabacumL. var Virginia Against Nosocomial Bacteria Pathogen

Cited by 11 publications

References 3 publications

Isolation of piperine from black pepper (Piper nigrum) in the provision of standard compounds for natural chemical practice and research activities

Isolation of piperine from black pepper (Piper nigrum) in the provision of standard compounds for natural chemical practice and research activities

Sustained release of alginate hydrogel containing antimicrobial peptide Chol-37(F34-R) in vitro and its effect on wound healing in murine model of Pseudomonas aeruginosa infection

Enhancing the antibacterial activity of antimicrobial peptide PMAP-37(F34-R) by cholesterol modification

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