In vitro Assay of Factors Affecting the Growth of Pathogens Associated with Diseases on Dragon Fruit (Hylocereus spp.) in Peninsular Malaysia

Masyahit, Masanto; Sijam, Kamaruzaman; Awang, Yahya; Satar, Mohd Ghazali Mohd

doi:10.3923/ppj.2009.144.151

Cited by 7 publications

(7 citation statements)

References 30 publications

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“…We did not focus on the biofilm form of growth in this study, as neither Pandoraea species examined has the ability to form biofilms (Caraher et al, 2008). Masyahit et al (2009) showed that B. multivorans displayed the greatest capacity for fungal inhibition in comparison to B. cenocepacia and Ps. aeruginosa.…”

Section: Inhibition Among Co-colonizing Cf Pathogensmentioning

confidence: 99%

Inhibition of co-colonizing cystic fibrosis-associated pathogens by Pseudomonas aeruginosa and Burkholderia multivorans

et al. 2014

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Cystic fibrosis (CF) is a recessive genetic disease characterized by chronic respiratory infections and inflammation causing permanent lung damage. Recurrent infections are caused by Gramnegative antibiotic-resistant bacterial pathogens such as Pseudomonas aeruginosa, Burkholderia cepacia complex (Bcc) and the emerging pathogen genus Pandoraea. In this study, the interactions between co-colonizing CF pathogens were investigated. Both Pandoraea and Bcc elicited potent pro-inflammatory responses that were significantly greater than Ps. aeruginosa. The original aim was to examine whether combinations of pro-inflammatory pathogens would further exacerbate inflammation. In contrast, when these pathogens were colonized in the presence of Ps. aeruginosa the pro-inflammatory response was significantly decreased. Real-time PCR quantification of bacterial DNA from mixed cultures indicated that Ps. aeruginosa significantly inhibited the growth of Burkholderia multivorans, Burkholderia cenocepacia, Pandoraea pulmonicola and Pandoraea apista, which may be a factor in its dominance as a colonizer of CF patients. Ps. aeruginosa cell-free supernatant also suppressed growth of these pathogens, indicating that inhibition was innate rather than a response to the presence of a competitor. Screening of a Ps. aeruginosa mutant library highlighted a role for quorum sensing and pyoverdine biosynthesis genes in the inhibition of B. cenocepacia. Pyoverdine was confirmed to contribute to the inhibition of B. cenocepacia strain J2315. B. multivorans was the only species that could significantly inhibit Ps. aeruginosa growth. B. multivorans also inhibited B. cenocepacia and Pa. apista. In conclusion, both Ps. aeruginosa and B. multivorans are capable of suppressing growth and virulence of co-colonizing CF pathogens.

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Section: Inhibition Among Co-colonizing Cf Pathogensmentioning

confidence: 99%

Inhibition of co-colonizing cystic fibrosis-associated pathogens by Pseudomonas aeruginosa and Burkholderia multivorans

et al. 2014

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“…The genus Burkholderia has been proven to be a very efficient biocontrol agent against several fungal and bacterial diseases in recent times [1,2]. Various species of Burkholderia, primarily B. cepacia, have been found to exhibit inhibitory activity against many potential pathogens [3][4][5][6][7][8][9]. Burkholderia species have been a source of many potentially bioactive products, some possessing antibacterial activity [9,10].…”

mentioning

confidence: 99%

Two new Aromatic Glycosides from a Soil Bacterium Burkholderia gladioli OR1

Khan

Bharti

Saraf

et al. 2016

Natural Product Communications

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Two new aromatic glycosides, named as gladioside I (1) and II (2) were isolated from the culture broth of a soil bacterium, Burkholderia gladioli OR1. Both 1 and 2 contained one unit each of rhamnose and 3- O-methyl xylose and differed from each other in the aglycone part. Compound 1 contained an aromatic aldehyde and compound 2 contained a styrylcarbamate unit as the aglycone part. The structures of 1 and 2 were elucidated by detailed spectral analysis and chemical degradation.

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“…For example, Rahman et al (2007) reported occurrence of antagonistic Pseudomonas aeruginosa strains from the fructospehere of papaya against C. gloeosporioides. Masyahit et al (2009) also reported the antagonistic association between Pseudomonas aeruginosa and C. gloeosporioides in dragon fruit. There are many other reports on the efficacy of Pseudomonas aeruginosa as a potential biocontrol agent for various phytopathogenic fungi (Siddiqui and Shaukat 2002, Mansoor et al 2007, Prakob et al 2009, Deshwal 2012, Maiti et al 2012.…”

Section: Discussionmentioning

confidence: 91%

Isolation, screening and in vitro evaluation of bacterial antago-nists from spent mushroom substrate against Colletotrichum musae

Ranathunge

Rajapaksha

Yogarajah

et al. 2016

Trop Agric Res & Ext

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Colletotrichum musae is a major pathogen in banana fruit which causes heavy losses at the postharvest stage. As a viable alternative to harmful chemical usage and physical control measures, the potential of using bacterial antagonist against C. musae was evaluated in this study. Out of a total of 92 bacterial isolates derived from dilution plates of aqueous extracts of spent mushroom substrate (SMS), 43 isolates were initially selected for screening for antagonistic activity against the C. musae isolate CMK01. Screening of the selected bacterial isolates revealed that 39 of the tested isolates significantly inhibited fungal growth in vitro compared to the control (P <0.05). The diffusible substances secreted by the five selected isolates showed 100% inhibition of colony growth, while (13-58%) inhibition was observed with volatile compounds. Light microscopic observations revealed deformation and blackening of C. musae hyphal tips in the colony margins facing the antagonists. Four of the selected five bacterial isolates were identified with 100% precision as Pseudomonas aeruginosa based on partial 16S rDNA sequences. These promising antagonists have the potential to be developed as effective biocontrol agents against C. musae.

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In vitro Assay of Factors Affecting the Growth of Pathogens Associated with Diseases on Dragon Fruit (Hylocereus spp.) in Peninsular Malaysia

Cited by 7 publications

References 30 publications

Inhibition of co-colonizing cystic fibrosis-associated pathogens by Pseudomonas aeruginosa and Burkholderia multivorans

Inhibition of co-colonizing cystic fibrosis-associated pathogens by Pseudomonas aeruginosa and Burkholderia multivorans

Two new Aromatic Glycosides from a Soil Bacterium Burkholderia gladioli OR1

Isolation, screening and in vitro evaluation of bacterial antago-nists from spent mushroom substrate against Colletotrichum musae

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