In vitro assays suggest Shenqi Fuzheng Injection has the potential to alter melanoma immune microenvironment

Du, Juan; Cheng, Brian Chi‐Yan; Fu, Xiu‐Qiong; Su, Tao; Li, Ting; Guo, Hui; Li, Sumei; Wu, Jinfeng; Yu, Hua; Huang, Weichao; Cao, Hui; Yu, Zhi‐Ling

doi:10.1016/j.jep.2016.08.038

Cited by 23 publications

(16 citation statements)

References 24 publications

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“…This also indicates that SFI exerts its effects by regulating the expression of the VEGF gene. Our results further confirmed the beneficial effects of SFI on the VEGF expression in consistence with previous report [23]. Although SFI is widely in China as an adjuvant drug for many cancers, such as lung and gastric cancers, the underlying mechanism is barely known.…”

Section: Discussionsupporting

confidence: 92%

Shenqi Fuzheng Injection (SFI) Enhances IFN-α Inhibitory Effect on Hepatocellular Carcinoma Cells by Reducing VEGF Expression: Validation by Gene Silencing Technique

Chen

et al. 2019

BioMed Research International

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Shenqi Fuzheng Injection (SFI) is a traditional Chinese medicine injection with anticancer properties and is mainly composed of ginseng and astragalus. Its efficacy has been confirmed in clinical trials, but the mechanism remains unclear. We investigated the effect of SFI on vascular endothelial growth factor (VEGF) gene expression in hepatocellular carcinoma (HCC) cells and identified its possible mechanism of synergistic effects when combined with the chemotherapeutic drug interferon (IFN-) α. An MTT assay was used to measure the inhibition effects of low-dose IFN-α (6000 IU) with or without SFI (0.5 g/L) on the HCC cell line MHCC97. VEGF-silenced MHCC97L-mir200 cell lines were prepared using lentiviral vectors and evaluated by real-time PCR to determine the inhibition effect. We examined MHCC97L-mir200 and MHCC97L cells by MTT assay, using IFN-α alone or in combination with SFI. The inhibition ratio of IFN-α (6000 IU) was -29.5%, while that for IFN-α (6000 IU) + SFI (0.5 g/L) was 17.0%, which was significantly higher than that for the IFN-α group (P < 0.01). The VEGF gene was silenced successfully in MHCC97-L cells. After interference of VEGF, the inhibition by SFI and IFN-α in MHCC97L-mir200 did not differ from that in MHCC97-L cells (P > 0.05). SFI can reduce the expression of VEGF in HCC, which can increase the efficacy of IFN-α, providing a theoretical basis for clinical application.

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Section: Discussionsupporting

confidence: 92%

Shenqi Fuzheng Injection (SFI) Enhances IFN-α Inhibitory Effect on Hepatocellular Carcinoma Cells by Reducing VEGF Expression: Validation by Gene Silencing Technique

Chen

et al. 2019

BioMed Research International

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“…In YFD/cisplatin combination treated group, the tumor cell density was further decreased and the invasion of inflammatory cell was more significant. These results indicate that YFD enhances anti-tumor effect by improving immune response to tumor tissues, consistent with the documented immune activation effect of Radix Astragali and Radix Codonopsis 10 , 16 . It is important to note that YFD is composed of 12 Chinese medicinal herbs.…”

Section: Discussionsupporting

confidence: 86%

Yangyin Fuzheng Decoction enhances anti-tumor efficacy of cisplatin on lung cancer

Wei¹,

Wang²,

Chen³

et al. 2018

J. Cancer

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Traditional Chinese medicine has been widely used in cancer treatment in China. Yangyin Fuzheng Decoction is a traditional Chinese compound medicine, composed of 12 traditional Chinese herbs. This study aimed to investigate anti-tumor activity and the underlying mechanisms of Yangyin Fuzheng Decoction combined with cisplatin in the treatment of lung cancer. We established lung cancer model in C57BL/6 mice injected with mouse Lewis lung cancer cells. Our results demonstrated that Yangyin Fuzheng Decoction treatment increased necrotic area in tumor tissue, and significantly enhanced the recruitment of inflammatory cells into the tumor. In addition, Yangyin Fuzheng Decoction treatment enhanced the anti-tumor efficacy of cisplatin and partially recovered mouse body weight loss caused by cisplatin treatment. Mechanistically, we found that Yangyin Fuzheng Decoction upregulated the expression of pro-apoptotic proteins p53 and Bax and suppressed the expression of anti-apoptotic protein Bcl-2. Combined treatment of Yangyin Fuzheng Decoction and cisplatin further increased p53 and Bax levels and suppressed Bcl-2 level. Taken together, these data suggest that Yangyin Fuzheng Decoction could synergistically enhance the apoptotic signaling in cancer cells during chemotherapy. In addition, it has health improving and immune response enhancing effects. Yangyin Fuzheng Decoction could be a promising adjunct agent for lung cancer chemotherapy.

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“…The TME enables a large number of regulatory T cells (Tregs) that penetrate and accumulate in tumor tissues, inhibit the differentiation and maturation of effector cells such as lymphocytes, macrophages, dendritic cells (DC), and isolate them from tumor tissues to inhibit immune responses. The immunosuppressive microenvironment is closely related to the “deficiency of vital Qi” in Chinese medicine ( 48 ). The “syndrome” of TCM involves multiple systems and levels of Western medicine.…”

Section: Introductionmentioning

confidence: 99%

Research Status and Molecular Mechanism of the Traditional Chinese Medicine and Antitumor Therapy Combined Strategy Based on Tumor Microenvironment

Zhang

Lou

Wang³

et al. 2021

Front. Immunol.

117

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Treatment of malignant tumors encompasses multidisciplinary comprehensive diagnosis and treatment and reasonable combination and arrangement of multidisciplinary treatment, which is not a simple superimposition of multiple treatment methods, but a comprehensive consideration of the characteristics and specific conditions of the patients and the tumor. The mechanism of tumor elimination by restoring the body’s immune ability is consistent with the concept of “nourishing positive accumulation and eliminating cancer by itself” in traditional Chinese medicine (TCM). The formation and dynamic changes in the tumor microenvironment (TME) involve many different types of cells and multiple signaling pathways. Those changes are similar to the multitarget and bidirectional regulation of immunity by TCM. Discussing the relationship and mutual influence of TCM and antitumor therapy on the TME is a current research hotspot. TCM has been applied in the treatment of more than 70% of cancer patients in China. Data have shown that TCM can significantly enhance the sensitivity to chemotherapeutic drugs, enhance tumor-suppressing effects, and significantly improve cancer-related fatigue, bone marrow suppression, and other adverse reactions. TCM treatments include the application of Chinese medicine monomers, extracts, classic traditional compound prescriptions, listed compound drugs, self-made compound prescriptions, as well as acupuncture and moxibustion. Studies have shown that the TCM functional mechanism related to the positive regulation of cytotoxic T cells, natural killer cells, dendritic cells, and interleukin-12, while negatively regulating of regulatory T cells, tumor-associated macrophages, myeloid-derived suppressive cells, PD-1/PD-L1, and other immune regulatory factors. However, the application of TCM in cancer therapy needs further study and confirmation. This article summarizes the existing research on the molecular mechanism of TCM regulation of the TME and provides a theoretical basis for further screening of the predominant population. Moreover, it predicts the effects of the combination of TCM and antitumor therapy and proposes further developments in clinical practice to optimize the combined strategy.

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In vitro assays suggest Shenqi Fuzheng Injection has the potential to alter melanoma immune microenvironment

Cited by 23 publications

References 24 publications

Shenqi Fuzheng Injection (SFI) Enhances IFN-α Inhibitory Effect on Hepatocellular Carcinoma Cells by Reducing VEGF Expression: Validation by Gene Silencing Technique

Shenqi Fuzheng Injection (SFI) Enhances IFN-α Inhibitory Effect on Hepatocellular Carcinoma Cells by Reducing VEGF Expression: Validation by Gene Silencing Technique

Yangyin Fuzheng Decoction enhances anti-tumor efficacy of cisplatin on lung cancer

Research Status and Molecular Mechanism of the Traditional Chinese Medicine and Antitumor Therapy Combined Strategy Based on Tumor Microenvironment

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