In vitro assembly of thermostable Csm complex in CRISPR–Cas type III/A system

Park, Kwang Hyun; An, Yan; Woo, Eui-Jeon

doi:10.1016/bs.mie.2018.10.025

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…The target sequence located upstream of the protospacer-associated motif (PAM), NGG, in the case of CRISPR/Cas9, was designed. The system has been widely used in plants for characterization of gene function and precision plant breeding [ 20 , 21 , 22 ].…”

Section: Genome-editing Technologymentioning

confidence: 99%

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

Fuhrmann-Aoyagi

et al. 2022

CIMB

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Global warming and climate change have severely affected plant growth and food production. Therefore, minimizing these effects is required for sustainable crop yields. Understanding the molecular mechanisms in response to abiotic stresses and improving agricultural traits to make crops tolerant to abiotic stresses have been going on unceasingly. To generate desirable varieties of crops, traditional and molecular breeding techniques have been tried, but both approaches are time-consuming. Clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALENs) are genome-editing technologies that have recently attracted the attention of plant breeders for genetic modification. These technologies are powerful tools in the basic and applied sciences for understanding gene function, as well as in the field of crop breeding. In this review, we focus on the application of genome-editing systems in plants to understand gene function in response to abiotic stresses and to improve tolerance to abiotic stresses, such as temperature, drought, and salinity stresses.

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Section: Genome-editing Technologymentioning

confidence: 99%

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

Fuhrmann-Aoyagi

et al. 2022

CIMB

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“…Recently, natural active compounds originating from medicinal plants have been exploited as promising antifungal and antibiofilm agents against C. albicans , owing to their abundant sources ( Atanasov et al., 2021 ). Some studies have suggested that natural plant compounds affect important biofilm virulence factors, including the inhibition of biofilm formation, adhesion, promotion of biofilm cell detachment, and dispersion of established mature biofilms ( Lu et al., 2019 ; Park et al., 2019 ). Similar to classical antifungal drugs, these natural compounds exert anti- Candida effects by generating reactive oxygen species (ROS) and targeting Candida cellular metabolic pathways, including ABC transporter mediated drug efflux, cell cycle progression, mitochondrial activity, and ergosterol, chitin, and cell wall biosynthesis ( Shariati et al., 2022 ; Campos Péret et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Isobavachalcone exhibits antifungal and antibiofilm effects against C. albicans by disrupting cell wall/membrane integrity and inducing apoptosis and autophagy

Qian,

Lu,

Gao

et al. 2024

Front. Cell. Infect. Microbiol.

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Isobavachalcone (IBC) is a natural flavonoid with multiple pharmacological properties. This study aimed to evaluate the efficacy of IBC against planktonic growth and biofilms of Candida albicans (C. albicans) and the mechanisms underlying its antifungal action. The cell membrane integrity, cell metabolic viability, and cell morphology of C. albicans treated with IBC were evaluated using CLSM and FESEM analyses. Crystal violet staining, CLSM, and FESEM were used to assess the inhibition of biofilm formation, as well as dispersal and killing effects of IBC on mature biofilms. RNA-seq combined with apoptosis and autophagy assays was used to examine the mechanisms underlying the antifungal action of IBC. IBC exhibited excellent antifungal activity with 8 μg/mL of MIC for C. albicans. IBC disrupted the cell membrane integrity, and inhibited biofilm formation. IBC dispersed mature biofilms and damaged biofilm cells of C. albicans at 32 μg/mL. Moreover, IBC induced apoptosis and autophagy-associated cell death of C. albicans. The RNA-seq analysis revealed upregulation or downregulation of key genes involved in cell wall synthesis (Wsc1 and Fks1), ergosterol biosynthesis (Erg3, and Erg11), apoptisis (Hsp90 and Aif1), as well as autophagy pathways (Atg8, Atg13, and Atg17), and so forth, in response to IBC, as evidenced by the experiment-based phenotypic analysis. These results suggest that IBC inhibits C. albicans growth by disrupting the cell wall/membrane, caused by the altered expression of genes associated with β-1,3-glucan and ergosterol biosynthesis. IBC induces apoptosis and autophagy-associated cell death by upregulating the expression of Hsp90, and altering autophagy-related genes involved in the formation of the Atg1 complex and the pre-autophagosomal structure. Together, our findings provide important insights into the potential multifunctional mechanism of action of IBC.

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Crystal structure of the Csm5 subunit of the type III-A CRISPR-Cas system

Park

Kim

et al. 2020

Biochemical and Biophysical Research Communications

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In vitro assembly of thermostable Csm complex in CRISPR–Cas type III/A system

Cited by 4 publications

References 17 publications

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

Isobavachalcone exhibits antifungal and antibiofilm effects against C. albicans by disrupting cell wall/membrane integrity and inducing apoptosis and autophagy

Crystal structure of the Csm5 subunit of the type III-A CRISPR-Cas system

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