In vitro assessment of a new ABO immuno-sorbent with synthetic carbohydrates attached to sepharose

Abstract

In vitro assessment of a new A60 immunosorbent with synthetic carbohydrates attached to sepharoseAbstract Transplantation across the ABO barrier is sometimes done in cases of emergency, such as acute liver failure, but is also carried out in elective cases, e.g. kidneys from living donors. Reducing the recipient anti-A/B antibody titres is often necessary in ABO-incompatible kidney transplantation. This is usually done by the use of techniques such as plasmapheresis and protein A-or sepharose-linked anti-human Ig immunoadsorption. A new ABO immunosorbent with synthetic A-or B-trisaccharide carbohydrate epitopes linked to a sepharose matrix has been tested. Columns made of this material have been tested in vitro with plasma from Aand B-individuals, assessed for antibody reduction capacity, flow characteristics, biocompatibility, and unspecific protein adsorption. The columns have a high capacity for ABO antibody removal, reducing titres by three to seven steps in one passage. We noted a high biocompatibility, with no unspecific protein adsorption, no activation of coagulation factors, and a low activation of complement, no immune complex formation and no cytotoxicity towards cultured mammalian L929 cells.

Selective Capture of Anti‐N‐glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate

“…[ 1 , 2 ] Historically, polymeric scaffolds based on carbohydrate epitopes have been used as immunosorbents, acting as “autoantibody scavengers” to remove pathogenic antibodies, particularly IgMs, from patient sera. [ 3 , 4 , 5 ] Various grafting strategies enable control of the overall architecture of the final grafted polymer, integrating precise chemical structures with diverse functionalities, yielding novel synthetic polymeric multivalent conjugates. The stability and processability of these bioinspired polymers enable their use as surrogates of the mimicked molecules, featuring similar functions and occasionally exceeding the performance because of multivalent presentation.…”

Efficient Conjugation of Oligosaccharides to Polymer Particles through Furan/Maleimide Diels–Alder Reaction: Application to the Capture of Carbohydrate‐Binding Proteins

“…The depletion of anti‐glycan antibodies in sera is beneficial for therapeutic developments and analytical studies of autoimmune diseases, such as the Guillain–Barré syndrome and Crohn's disease . Carbohydrate‐based immunoadsorbents have been shown to be efficient and safe in organ transplantation across the ABO blood group barrier . They also hold promise for improving universal blood plasma supply .…”