In vitro assessment of Argemone mexicana, Taraxacum officinale, Ruta chalepensis and Tagetes filifolia against Haemonchus contortus nematode eggs and infective (L3) larvae

Díaz, Gabriela Jasso; Hernández, Glafiro Torres; Zamilpa, Alejandro; Pérez, Carlos M.; Bribiesca, J. Efrén Ramírez; Hernández–Mendo, Omar; Arroyo, Hussein Sánchez; Cortazar, Manasés González; Gives, Pedro Mendoza de

doi:10.1016/j.micpath.2017.05.048

Cited by 32 publications

(15 citation statements)

References 16 publications

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“…It has been reported to have an anti-inflammatory effect and may improve blood flow and mitotic activity in traumatized rat livers [ 32 ]. In addition, its antimicrobial and antifungal activities have beedn reported [ 17 ]. The activity of this alkaloid was also reported in vitro against P. falciparum , Leishmania donovani , and Trypanosoma brucei rhodesiense [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Study of Antiamoebic Activity of Methanol Extracts ofArgemone mexicanaon Trophozoites ofEntamoeba histolyticaHM1-IMSS

Elizondo-Luévano

Castro‐Ríos

Sánchez

et al. 2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Infections caused by parasites in humans represent one of the main public health concerns. Amoebiasis, a parasitic infection caused by Entamoeba histolytica (E. histolytica), is considered endemic in Mexico, where Argemone mexicana (A. mexicana) has been used in traditional medicine to treat intestinal parasitic diseases. The objective of this work was to evaluate the potential biological activity of A. mexicana on E. histolytica. For this purpose, a methanolic extract was prepared from A. mexicana leaves, and a differential fractionation was carried out with solvents of different polarities. The inhibitory capacities of the extract and its fractions were evaluated in vitro using HM1-IMSS, a strain of Entamoeba histolytica. A. mexicana extract was found to have a growth-inhibiting activity for E. histolytica, showing IC50 = 78.39 μg/mL. The extract was characterized phytochemically, and the methanolic extract fractions were analyzed by liquid chromatography (HPLC) and mass spectrometry (MS). Berberine and jatrorrhizine were present in the active fractions, and these compounds may be responsible for the antiparasitic activity. The identification of amoebicidal activity of A. mexicana on E. histolytica gives support to the traditional use. Further studies with berberine and jatrorrhizine will be carried out to understand the mechanism involved.

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Section: Resultsmentioning

confidence: 99%

In Vitro Study of Antiamoebic Activity of Methanol Extracts ofArgemone mexicanaon Trophozoites ofEntamoeba histolyticaHM1-IMSS

Elizondo-Luévano

Castro‐Ríos

Sánchez

et al. 2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Few investigations have been carried out to determine the effect of phenylpropanoids on parasitic nematodes and data on nematicidal action are restricted to a small number of studies on fish, dog and swine parasites [27]. Recently, some phenylpropanoids have been evaluated with regard to the effects on nematodes of ruminant parasites; caffeic acid, p-coumaric acid, ferulic acid, methyl caffeate, methyl p-coumarate and methyl ferulate were identified as having ovicidal action [36] and chlorogenic acid was identified as having both ovicidal and larvicidal action [37] against H . contortus .…”

Section: Discussionmentioning

confidence: 99%

Discovery of potential ovicidal natural products using metabolomics

et al. 2019

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Plant extracts are a potential source of new compounds for nematode control and may be an excellent alternative for the control gastrointestinal nematodes that are resistant to conventional anthelmintics. However, research involving natural products is a complex process. The main challenge is the identification of bioactive compounds. Online analytical techniques with universal detectors, such as high-performance liquid chromatography-mass spectrometry (HPLC-MS), together with metabolomics could enable the fast, accurate evaluation of a massive amount of data, constituting a viable option for the identification of active compounds in plant extracts. This study focused on the evaluation of the ovicidal activity of ethanol extracts from 17 plants collected from the Pantanal wetland in the state of Mato Grosso do Sul, Brazil, against eggs of Haemonchus placei using the egg hatchability test. The ethanol extracts were obtained using accelerated solvent extraction. The data on ovicidal activity, mass spectrometry and metabolomics were evaluated using HPLC-DAD-MS, partial least squares regression analysis (PLS-DA) and a correlation map (univariate correlation analyses) to detect compounds that have a positive correlation with biological activity. Among the ten metabolites with the best correlation coefficients, six were phenylpropanoids, two were triterpene saponins, one was a brevipolide, and one was a flavonoid. Combinations of metabolites with high ovicidal action were also identified, such as phenylpropanoids combined with the triterpene saponins and the flavonoid, flavonoids combined with iridoid and phenylpropanoids, and saponins combined with phenylpropanoid. The positive correlation between classes of compounds in plants belonging to different genera and biological activity (as previously identified in the literature) reinforces the robustness of the statistical data and demonstrates the efficacy of this method for the selection of bioactive compounds without the need for isolation and reevaluation. The proposed method also enables the determination of synergism among the classes, which would be impracticable using traditional methods. The present investigation demonstrates that the metabolomic technique was efficient at detecting secondary metabolites with ovicidal activity against H. placei. Thus, the use of metabolomics can be a tool to accelerate and simplify bioprospecting research with plant extracts in veterinary parasitology.

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“…Using bioassay-guided purification from Tagetes filifolia, chlorogenic acid proved to be the anthelmintic compound (LC 50 248 µg/mL) in an in vitro test (egg hatching or mortality of H. contortus larvae) [80].…”

Section: Natural Products Active Against Intestinal Parasitic Nematodesmentioning

confidence: 99%

“…They isolated anthelmintic compounds such as epicatechin (EC 50 = 10 µg/mL) with higher efficacy than rutin (EC 50 = 30 µg/mL). Additionally, chlorogenic acid was also isolated and tested but did not show significant effects, even though Jasso Diaz et al [80] previously found (albeit weak) anthelmintic activity.…”

Section: Natural Products Active Against Intestinal Parasitic Nematodesmentioning

confidence: 99%

Plant-Based Natural Products for the Discovery and Development of Novel Anthelmintics against Nematodes

2020

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Intestinal parasitic nematodes infect approximately two billion people worldwide. In the absence of vaccines for human intestinal nematodes, control of infections currently relies mainly on chemotherapy, but resistance is an increasing problem. Thus, there is an urgent need for the discovery and development of new anthelmintic drugs, especially ones with novel mechanisms of action. Medicinal plants hold great promise as a source of effective treatments, including anthelmintic therapy. They have been used traditionally for centuries and are mostly safe (if not, their toxicity is well-known). However, in most medicinal plants the compounds active against nematodes have not been identified thus far. The free-living nematode C. elegans was demonstrated to be an excellent model system for the discovery of new anthelmintics and for characterizing their mechanism of action or resistance. The compounds discussed in this review are of botanical origin and were published since 2002. Most of them need further studies of their toxicity, mechanisms and structure-activity relationship to assess more fully their potential as drugs.

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In vitro assessment of Argemone mexicana, Taraxacum officinale, Ruta chalepensis and Tagetes filifolia against Haemonchus contortus nematode eggs and infective (L3) larvae

Cited by 32 publications

References 16 publications

In Vitro Study of Antiamoebic Activity of Methanol Extracts ofArgemone mexicanaon Trophozoites ofEntamoeba histolyticaHM1-IMSS

In Vitro Study of Antiamoebic Activity of Methanol Extracts ofArgemone mexicanaon Trophozoites ofEntamoeba histolyticaHM1-IMSS

Discovery of potential ovicidal natural products using metabolomics

Plant-Based Natural Products for the Discovery and Development of Novel Anthelmintics against Nematodes

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