In vitro assessment of functional properties of lactic acid bacteria isolated from faecal microbiota of healthy dogs for potential use as probiotics

Abstract

Lactic acid bacteria were isolated and identified in the faeces of Chinese Crested and Yorkshire terrier pups and their probiotic features were investigated in vitro. Thirty seven isolates were identified as Lactobacillus or Enterococcus. Out of these isolates, 31 were lactic acid bacteria (LAB) and belonged to the species Lactobacillus reuteri (16/37; 43.3%), Lactobacillus animalis (7/37; 18.9%), Lactobacillus acidophilus (3/37; 8.1%), Lactobacillus sanfranciscensis (2/37; 5.4%), Lactobacillus murinus (2/37; 5.4%), and Lactobacillus paraplantarum (1/37; 2.7%), while six other LAB isolates were Enterococcus spp. (6/37; 16.2%). Strains were tested for resistance to gastric acidity (pH 2.5 for 3 h) and bile salts (0.3% ox gall), cell surface hydrophobicity by microbial adhesion to solvents, antagonism against pathogenic bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Salmonella enterica serovar Typhimurium and Listeria monocytogenes), production of hydrogen peroxide, and antibiotic susceptibility. Thirty four strains were highly resistant to acidic conditions with slight (18 strains) to moderate (16 strains) growth inhibition by bile salts. Seven isolates had highly hydrophobic cellular surfaces and 28 strains exhibited strong antagonism against the bacterial pathogens tested, although 8 isolates tested against Leptospira interrogans had no effect on pathogen growth. All isolates produced low rates of hydrogen peroxide. Based on these results, two Lactobacillus strains showed promising probiotic-related features and merit investigation as probiotics for dogs.

Modeling lactation curve for genetic evaluation of Murrah buffaloes

“…The inhibition zones were large and clear against the reference strains of E. coli (ATCC 25922), K. pneumoniae (ATCC 700603), S. typhimurium (MTCC 3231) and S. enteritidis (ATCC 13076) but limited and hazy zones were observed against Enterobacter species. Silva et al (2013) from Brazil reported promising in-vitro antibacterial activity in Lactobacillus isolates of dog faecal origin against E. coli and other pathogenic bacteria. Vineetha et al (2016) from India reported promising in-vitro antibacterial activity in Lactobacillus isolates of poultry faecal origin against E. coli, S. typhimurium and S. enteritidis.…”

Assessment of the probiotic potential of lactic acid bacteria isolated from Minas artisanal cheese produced in the Campo das Vertentes region, Brazil

“…LAB intrinsically encoding resistance to oxacillin has also been well described in the literature, considering that this antimicrobial is not related to plasmid conjugation (Mathur and Singh ; Silva et al . ; Alvim et al . ).…”

Microbiota and probiotics in canine and feline welfare

“…Lactobacillus fermentum, Lactobacillus rhamnosus, and Lactobacillus salivarius are reported being a part of health canine intestine [13]. Other canine lactobacilli are represented by Lactobacillus murinus and Lactobacillus reuteri [12], Lactobacillus animalis, Lactobacillus sanfranciscensis and Lactobacillus paraplantarum [14].…”