In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing

Abstract

Advanced platelet-rich fibrin (A-PRF) induces more proliferation and migration of fibroblasts compared with standard PRF, but it being freshly prepared prior to it being applied is necessary. Therefore, this study aimed to determine the effect of lyophilized A-PRF on growth factor release and cell biological activity. Blood samples were collected from six dogs and processed for fresh and lyophilized A-PRF. The growth factors released included transforming growth factor beta-1 (TGF-β1), vascular endothelial growth factor-A (VEGFA), and platelet-derived growth factor-BB (PDGF-BB), and the fibroblast proliferation as well as wound closure enhancement of both products were compared. The results showed that TGF-β1, PDGF-BB, and VEGFA were continually released from lyophilized A-PRF for over 72 h. Lyophilized A-PRF released significantly more accumulated VEGEA and a tendency to release more TGF-β1 at 72 h as well as VEGFA at 24 h and 72 h than fresh A-PRF. Moreover, lyophilized A-PRF increased fibroblast proliferation and induced a significantly faster wound closure than the control, while no significant difference between fresh and lyophilized A-PRF was found. In conclusion, the lyophilization of canine A-PRF can preserve the release of growth factors and has similar biological activities to a fresh preparation. This encourages the substitution of lyophilized A-PRF instead of fresh A-PRF in regenerative treatments in which the stability of the product is concerned.

Short-Term Effects of Two COX-2 Selective Non-Steroidal Anti-Inflammatory Drugs on the Release of Growth Factors and Cytokines from Canine Platelet-Rich Gel Supernatants

“…PDGF-BB and TGF-β 1 were selected in the present study because these mediators are mainly found in platelets. They are directly involved in the processes of inflammation resolution, chemotaxis, and neovascularization [ 30 , 31 ]. All of these actions are necessary to induce wound healing and subsequent regeneration.…”

In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich Fibrin

“…The study involved six healthy Labrador retriever dogs (three males and three females) from Border Patrol Police Division 33, Chiang Mai, Thailand. As previously reported, the dogs were evaluated for their health status and management [23]. The experiment involved collecting and processing 10 mL of blood from each dog to prepare the canine PRF Animals 2023, 13, 3786 3 of 11 (cPRF), as described in [23].…”

“…As previously reported, the dogs were evaluated for their health status and management [23]. The experiment involved collecting and processing 10 mL of blood from each dog to prepare the canine PRF Animals 2023, 13, 3786 3 of 11 (cPRF), as described in [23]. The experiment compared the antimicrobial effects of three conditions: (1) growth control using phosphate buffer saline (PBS) (Gibco, Life Technologies Corporation, New York, NY, USA); (2) cPRF; and (3) drug control using antibiotics.…”

“…In contrast, Staphylococcus pseudintermedius is the most common bacterium in canine wounds [21,22]. Recent research has reported on the wound-healing property of canine platelet-rich fibrin (cPRF) in vitro and found that cPRF increased fibroblast cell proliferation and induced a significantly faster wound closure than the control [23]. However, no study has yet determined whether cPRF can effectively combat E. coli and S. pseudointermedius or how it does so.…”