In vitro assessment of metabolic profile of Enterococcus strains of human origin

Bhagwat, Ashlesha; Annapure, Uday S.

doi:10.1186/s43141-019-0009-0

Cited by 21 publications

(6 citation statements)

References 44 publications

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“…Los microorganismos que presentan capacidad antioxidante, mediante la producción de metabolitos y/o presencia de componentes en su superficie celular, podrían disminuir o anular el efecto negativo de los ROS (Bhagwat & Annapure, 2019). En este estudio, se evaluaron diferentes ensayos in vitro.…”

Section: Resultados Y Discusiónunclassified

Capacidad antioxidante de bacterias lácticas aisladas de peces e invertebrados marinos de la provincia de Chubut, Patagonia-Argentina

Sosa,

Parada,

Sánchez-Cabrera

et al. 2023

Rev. Cien. Mar. Cost.

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La actividad antioxidante de las bacterias ácido lácticas (BAL) ha sido estudiada en profundidad en cepas aisladas de organismos terrestres, alimentos fermentados y sin procesar. Sin embargo, esta información en aislamientos de origen marino aún está siendo determinada. Tal característica presenta un interés particular, debido a su uso potencial en alimentos funcionales. En este trabajo, se estudió la capacidad antioxidante de 16 BAL bacteriocinogénicas provenientes de organismos marinos de la costa del Chubut (periodo 2010-2022), mediante ensayos in vitro e in vivo. Se evaluó la capacidad antioxidante in vitro por medio de la reducción de 2,2-difenil-1-picrihidrazilo (DPPH), actividad antioxidante reductora de iones cúpricos (CUPRAC) y barrido de radicales oxidrilos, empleando células sin lisar. Se utilizó Saccharomyces cerevisiae como modelo biológico eucariota para valorar el efecto protector in vivo. Los resultados obtenidos por secuestro de radicales DPPH exhibieron valores entre 15-37.5%; el ensayo de secuestro de radicales oxidrilos mostró valores entre 13.3-61.9% y el método CUPRAC los valores variaron entre 0.223-0.551, equivalentes a mmol de ácido ascórbico por ml de suspensión celular. El efecto protector de las BAL en la supervivencia de S. cerevisiae presentó valores entre 36-90%. No fue posible establecer relaciones entre los valores obtenidos por distintos métodos o entre miembros del mismo género o especie; por ello, la capacidad antioxidante podría considerarse una característica dependiente de la cepa. Los resultados sugieren que la capacidad antioxidante de BAL bacteriocinogénicas de origen marino podría usarse como un carácter metabólico de selección con potencial biotecnológico en el desarrollo de alimentos funcionales.

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Section: Resultados Y Discusiónunclassified

Capacidad antioxidante de bacterias lácticas aisladas de peces e invertebrados marinos de la provincia de Chubut, Patagonia-Argentina

Sosa,

Parada,

Sánchez-Cabrera

et al. 2023

Rev. Cien. Mar. Cost.

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“…Liver derived conjugated BAs can be deconjugated in the small intestine by bile salt hydrolases (BSHs). These BSHs can be detected in gut microbiota ( 25 ) such as Lactobacillus spp ( 26 )., Bifidobacterium spp ( 27 )., Enterococcus spp ( 28 )., Clostridium spp ( 29 , 30 )., and Bacteroides spp ( 31 ).. More recent studies show that BSHs can be found in 591 intestinal bacterial strains within 117 genera in human gut microbiota. Notably, 27.52% of these bacterial strains contains only BSH paralogs ( 32 ).…”

Section: Effects Of Gut Microbiota On Basmentioning

confidence: 99%

Gut microbiota derived bile acid metabolites maintain the homeostasis of gut and systemic immunity

Gao

Yang

2023

Front. Immunol.

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Bile acids (BAs) as cholesterol-derived molecules play an essential role in some physiological processes such as nutrient absorption, glucose homeostasis and regulation of energy expenditure. They are synthesized in the liver as primary BAs such as cholic acid (CA), chenodeoxycholic acid (CDCA) and conjugated forms. A variety of secondary BAs such as deoxycholic acid (DCA) and lithocholic acid (LCA) and their derivatives is synthesized in the intestine through the involvement of various microorganisms. In addition to essential physiological functions, BAs and their metabolites are also involved in the differentiation and functions of innate and adaptive immune cells such as macrophages (Macs), dendritic cells (DCs), myeloid derived suppressive cells (MDSCs), regulatory T cells (Treg), Breg cells, T helper (Th)17 cells, CD4 Th1 and Th2 cells, CD8 cells, B cells and NKT cells. Dysregulation of the BAs and their metabolites also affects development of some diseases such as inflammatory bowel diseases. We here summarize recent advances in how BAs and their metabolites maintain gut and systemic homeostasis, including the metabolism of the BAs and their derivatives, the role of BAs and their metabolites in the differentiation and function of immune cells, and the effects of BAs and their metabolites on immune-associated disorders.

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“…Avirulent probiotic bacterial strains of E. canintestini S18A, E. rivorum S22C, and E. rivorum S14B previously isolated from human vagina and infants' meconium were used for the probiotic preparation by spray-drying. The bacterial strain sequences were deposited in NCBI GenBank under accession numbers KX 830979, KX 830971, and KX 830973, respectively [10][11][12]. The strains preserved in 40% glycerol stocks were inoculated in 100 ml of MRS broth and incubated for 48 h at 37 °C.…”

Section: Bacterial Cell and Probiotic Preparationmentioning

confidence: 99%

“…The present investigation involves the effect of encapsulation of three previously screened potential probiotic Enterococcus strains of human origin using a proteincarbohydrate complex as wall material and spray-drying method on viability [10,11].…”

Section: Introductionmentioning

confidence: 99%

Spray drying studies of probiotic Enterococcus strains encapsulated with whey protein and maltodextrin

Bhagwat

Bhushette

Annapure

2020

Beni-Suef Univ J Basic Appl Sci

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Background Probiotic Enterococcus strains of human origin were microencapsulated by spray drying using whey protein and maltodextrin as an encapsulating agent. The obtained encapsulates were characterized for stability, viability, and physiological properties. Results The microcapsules were prepared from probiotic Enterococcus strains that were previously isolated from human vagina and infants’ meconium. The microcapsules revealed similar particle sizes and morphologies. The highest hygroscopicity was observed in the microcapsules produced with strain E. rivorum S22C (0.17 ± 1.15) g water/kg powder/min. E. canintestini S18A revealed highest dissolution time in water (703 ± 2 s). The DSC thermogram revealed excellent thermal stability of all microcapsules. The physicochemical and morphological characteristics of the microcapsules were acceptable with regard to residual water content, particle mean size, and thermophysical properties and storage stability under room temperature conditions, with a low inactivation rate of Enterococcus strains. All the microcapsules revealed the recommended count of probiotic cells, low moisture content with low water activity. Observation under a scanning electron microscope revealed spherical-shaped partially collapsed structures measuring between 9 and 14 μm with surface concavities. Conclusions The microcapsule probiotic strains of Enterococcus microencapsulated by spray drying using whey protein and maltodextrin revealed properties of acceptable standards. These strains can have future potential as developing probiotic animal feed and food industry.

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In vitro assessment of metabolic profile of Enterococcus strains of human origin

Cited by 21 publications

References 44 publications

Capacidad antioxidante de bacterias lácticas aisladas de peces e invertebrados marinos de la provincia de Chubut, Patagonia-Argentina

Capacidad antioxidante de bacterias lácticas aisladas de peces e invertebrados marinos de la provincia de Chubut, Patagonia-Argentina

Gut microbiota derived bile acid metabolites maintain the homeostasis of gut and systemic immunity

Spray drying studies of probiotic Enterococcus strains encapsulated with whey protein and maltodextrin

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