In Vitro Assessment of Migratory Behavior of Two Cell Populations in a Simple Multichannel Microdevice

Abstract

Recent literature suggests that mesenchymal stem/stromal cells (MSC) could be used as Trojan Horses to deliver "death-signals" to cancer cells. Herein, we describe the development of a novel multichannel cell migration device, and use it to investigate the relative migration rates of bone marrow-derived MSC and breast cancer cells (MCF-7) towards each other. Confluent monolayers of MSC and MCF-7 were established in adjacent chambers separated by an array of 14 microchannels. Initially, culture chambers were isolated by air bubbles (air-valves) contained within each microchannel, and then bubbles were displaced to initiate the assay. The MCF-7 cells migrated preferentially towards MSC, whilst the MSC did not migrate preferentially towards the MCF-7 cells. Our results corroborate previous literature that suggests MSC migration towards cancer cells in vivo is in response to the associated inflammation rather than directly to signals secreted by the cancer cells themselves. OPEN ACCESSProcesses 2013, 1 350

Vascular tissue engineering: Fabrication and characterization of acetylsalicylic acid‐loaded electrospun scaffolds coated with amniotic membrane lysate

“…Moreover, at the early stages postimplantation, MSCs can participate in tissue remodeling processes such as ECM remodeling and paracrine signaling. The secretome of MSC is shown to be a rich source of trophic factors to many other cells (Kabiri, Lott, Kabiri, Russell, & Doran, ; Ranganath, Levy, Inamdar, & Karp, ). They can secrete multiple growth factors and proangiogenic cytokines such as VEGF, which, in turn, can recruit circulating progenitors of EC and SMC or induce the migration of more mature vascular cells in adjacent vessels (Watt et al, ).…”

“…The secretome of MSC is shown to be a rich source of trophic factors to many other cells (Kabiri, F I G U R E 8 Morphology of Wharton's jelly-derived MSCs seeded on the scaffolds after 7 days, shown in two different magnification. Aligned (a,b), Aligned-ASA (c,d), Random (e,f) and Random-ASA (g,h)Lott,Kabiri, Russell, & Doran, 2013;Ranganath, Levy, Inamdar, & Karp, 2012). They can secrete multiple growth factors and proangiogenic cytokines such as VEGF, which, in turn, can recruit circulating progenitors of EC and SMC or induce the migration of more mature vascular cells in adjacent vessels(Watt et al, 2013).…”

Effects of platelet rich plasma and chondrocyte co-culture on MSC chondrogenesis, hypertrophy and pathological responses

“…MSC have established trophy effects towards various cell types (Wu et al, 2012[ 44 ]; Kabiri et al, 2013[ 22 ]). MSC can help chondrocytes and get affected by chondrocytes.…”

Special Issue: Design of Bioreactor Systems for Tissue Engineering

“…Continuing the theme of the use of bioreactors as a research tool, Kabiri et al [3] describe how a fourteen channel bioreactor device can be used to study cell migration, and have used it to investigate the migration rates of bone marrow-derived MSC and MCF-7 breast cancer cells towards each other. Papantoniou et al [4] report their work on the application of Design of Experiments methodology to evaluate bioreactor operating conditions and the effects on osteogenic gene expression.…”