In vitro assessment of physiological traits and ROS detoxification pathways involved in tolerance of Damask rose genotypes under salt stress

Abstract

Rosa damascena is one of the most important medicinal and ornamental plants in Iran which is tolerant of salinity to some extent. However, the selection of genotypes that are more tolerant to salinity will influence on Damask cultivation in salt stress-affected regions. For this purpose, a factorial experiment in a completely randomized design with three replicates was performed under in vitro conditions on four Damask rose genotypes (Atashi, Bi-Khar, Chahar-Fasl and Kashan) at 5 concentrations of NaCl (0, 25, 50, 75, and 100 mM), and the physico-chemical traits were measured 14 and 28 days after treatment.The results showed that Atashi genotype with high levels of Chl a, Chl b, total Chl content, carotenoids, relative leaf water content, proline, total soluble protein, TPC, TFC, TAA, and the highest increase in the activity of antioxidant enzymes such as GPX, APX, CAT, SOD, and POD as well as the lowest amount of hydrogen peroxide showed a better protection mechanism against oxidative damage than the other three genotypes (Bi-Khar, Chahar-Fasl and Kashan) in the 14th and 28th days by maintaining the constructive and induced activities of antioxidant enzymes, it was shown that Bi-Khar genotype had moderate tolerance and Kashan and Chahar-Fasl genotypes had low tolerance to salinity stress. In vitro selection methods can be used effectively for salt tolerant screening of Damask rose genotypes, although the same experiment should be conducted in open filed cultures to verify the in vitro experimental results.

Biosynthesis of copper nanoparticles using Solenostemma argel and their effect on enhancing salt tolerance in barley plants

“…Numerous studies have indicated that exposure to salt stress can trigger the production of reactive oxygen species, leading to heightened activity of antioxidant enzymes as a protective mechanism 74 – 76 . This is consistent with our results, where the levels of antioxidant enzymes including SOD, CAT, APX, and GR activity significantly increased in barley plants as response to salt stress.…”

Metabolically Tailored Selection of Ornamental Rose Cultivars through Polyamine Profiling, Osmolyte Quantification and Evaluation of Antioxidant Activities

“…Extensive literature emphasizes the strong correlation between elevated endogenous PRO levels and its multifaceted role in increasing stress tolerance against various abiotic stressors, including salt stress [46], drought [47,48] and temperature extremes [7]. These functions are attributed to proline's antioxidant, osmoprotective and cryoprotective properties, along with its function as a chaperone and modulator of gene expression [49].…”

Melatonin application in in vitro conditions may modulate the phyto-biochemical mechanisms of Hymenocrater longiflorus Benth. under salinity stress

“…Based on the current study on the H. longi orus plant under in vitro culture, increasing NaCl in the medium enhanced the SOD, APX, and CAT enzyme activities. Previous reports have also indicated a drastic increase in antioxidant enzyme activities in the Damask rose genotype shoot cultures(Azizi et al 2023) and Leucojum aestivum plants obtained from embryogenic cells under salt stress(Ptak et al 2019). In addition, the present study demonstrated that, depending on the concentrations, melatonin led to a further increase in the activity of the SOD, POD, and CAT enzymes in plants exposed to salinity.…”