In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen

Salmani, Hossein; Towhidi, Armin; Zhandi, Mahdi; Bahreini, Majid; Sharafi, Mohsen

doi:10.1016/j.cryobiol.2014.02.008

Cited by 97 publications

(86 citation statements)

References 36 publications

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“…Furthermore, neither glycerol nor egg yolk alone could reduce the intact acrosome percentage; however, combination of these two protectants significantly reduces the percentage of intact acrosome spermatozoa [82]. Salmani et al [83] have suggested the use of soyabeanlecithin which is a suitable plant based cryoprotectant for caprine sperms. The beneficial effects of soyabean-lecithin as a substitute for egg yolk during cryopreservation of goat sperm are established in the works of Jiménez-Rabadán et al, Roof et al, Salmani et al, and Vidal et al [84][85][86][87].…”

Section: Cattlementioning

confidence: 99%

“…Samples were frozen in LN 2 vapor, 4 cm above the L, for 7 min; subsequently the straws were plunged into the LN 2 for storage. [83] Blanca-Celtiberica buck Diluted semen was cooled to 5 ∘ C for 2 h, diluted further, and held at 5 ∘ C for 2 h; another sample was cooled to 5 ∘ C for 4 h; both samples were frozen over N 2 vapour for 10 min, 4 cm above N 2 level, plunged, and stored in LN 2 .…”

Section: Goatmentioning

confidence: 99%

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Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Mandal

Badyakar

Chakrabarty

2014

Advances in Andrology

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Lipid is an important constituent of cell membrane. Membrane lipid composition of spermatozoa has been correlated to different function. Many researchers have related membrane lipid with survival success after cryopreservation or cold shock. Sperm maturation and acrosome reactions are natural phenomenon, but cryopreservation or cold shock is not. Therefore, sperm cells are not programmed for such change and undergo stress. So the change in membrane lipid composition due to cold shock or cryopreservation may be looked upon as response of spermatozoa to a certain stressed condition. A significant body of research worked on the relationship between membrane lipid and fatty acid composition and ability of cell to tolerate adverse change in temperature. However, as the approach of different research groups was different, it is very difficult to compare the changes. Studies have been done with different species, ejaculated/seminal or epididymal sperm. Lipid analyses have been done with whole cell membrane isolated by different methods. Fatty acids estimated were from whole cell, plasma membrane, head membrane, or phospholipids. The cryopreservation condition, media composition, and diluents/cryoprotectants were also different. At this onset a comprehensive review is needed to cover changes of sperm membrane lipid composition of different species under different cryopreservation conditions.

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Section: Cattlementioning

confidence: 99%

Section: Goatmentioning

confidence: 99%

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Mandal

Badyakar

Chakrabarty

2014

Advances in Andrology

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“…The basic extender was prepared by mixing 300 mM Tris, 28 mM glucose, 95 mM citric acid, 5% glycerol (v/v), and 500 µg/ ml gentamicin in distilled water respectively. Three different treatment extenders were prepared by adding 1%, 1.5% and 2% Soybean Lecithin (w/v) with 100 ml of basic extender and control extender was prepared by adding 15% egg yolk (Salmani et al, 2014). After preparation extender was kept in water bath at 37° C for semen dilution.…”

Section: Methodsmentioning

confidence: 99%

“…In similar to our findings, 1% SL was reported optimal for cryopreservation of ram (Sharafi et al, 2009 andForouzanfar et al, 2010) and human semen. Salmani et al (2014) observed increase of soybean lecithin level above 1.5% in the extender leads to reduce sperm quality after freezing-thawing process. Forouzanfar et al (2010) reported that high concentration of soybean lecithin was toxic for sperm motility and viability.…”

Section: Functional Membrane Integritymentioning

confidence: 99%

“…The first mechanism for protective effects of soybean lecithin is described by Bergeron and Manjunath (2006) suggested an interaction between seminal plasma proteins and LDL in extenders. Furthermore, the influential antioxidant compounds in soy lecithin as glutathione may protect the sperm viability via scavenging the LPO and prevention of MDA formation during cooling or freezing process (Salmani et al, 2014).…”

Section: Functional Membrane Integritymentioning

confidence: 99%

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Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender

Konyak

Mandal

Mondal

et al. 2018

IJAR

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This experiment was conducted with the aim to study the effect of replacing egg yolk with soybean lecithin (SL) for cryopreservation of Black Bengal buck semen. Sexually matured Black Bengal buck (n = 5) were used and the ejaculates were obtained using an artificial vagina method. The semen samples were pooled and diluted in Tris extender with 5% Glycerol containing either 15% egg yolk (control group) or SL at different concentrations (1% SL, 1.5% SL and 2% SL). The semen samples were filled in straws and cooled gradually to 5 °C. Semen straws were equilibrated for 3 hours at 5°C and were frozen in static liquid nitrogen vapor and stored in liquid nitrogen. Semen samples were evaluated after initial dilution, after completion of equilibration and after freeze thawing for in vitro sperm characters such as sperm motility, functional membrane integrity and malondioldehyde (MDA) concentration. Semen samples preserved in extender containing 1% SL was able to maintain in vitro sperm characters similar to the extender containing egg yolk. However, significant (P<0.05) reduction in all semen parameters was observed as the concentration of soybean lecithin increased above 1% level. It is concluded that an extender containing soybean lecithin @ 1% with 5% Glycerol can be used for replacing egg yolk for cryopreservation of Black Bengal buck semen.

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The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

Aktar,

Toker,

Koca

et al. 2024

Veterinary Medicine & Sci

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ObjectivesThis study aimed to examine the effects of supplementation of vitamin D to the egg‐yolk extender on characteristics of frozen–thawed ram semen.MethodsSemen samples obtained from adult rams were pooled and divided into five equal volumes. It was reconstituted with extenders containing different concentrations of vitamin D: 0 (control), 12.5 (VITD 12.5), 25 (VITD 25), 50 (VITD 50), and 100 ng/mL (VITD 100), and then they were frozen. Sperm motility parameters, plasma membrane functional integrity, acrosomal integrity, DNA fragmentation, and mitochondrial membrane potential of the groups were evaluated after sperm thawing.ResultsTotal motility and progressive motility were higher in VITD 50 than in all other groups (p < 0.05). Higher sperm straightness, linearity, and wooble were higher in VITD 50 than in the control group (p < 0.05). A similar pattern of VITD 50 was observed for plasma membrane integrity and mitochondrial membrane potential (p > 0.05).ConclusionsIn the study, it was observed that adding vitamin D to the extender had a beneficial effect on ram spermatological parameters. In addition, it was concluded that the use of the 50 ng/mL vitamin D in the extender provided more effective protection than the other doses.

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In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen

Cited by 97 publications

References 36 publications

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender

The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

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