In vitro Assessment of Total Bioactive Contents, Antioxidant, Anti-Alzheimer and Antidiabetic Activities of Leaves Extracts and Fractions of Aloe vera

Abstract

The present work was conducted to evaluate the quantitative analysis of the leaves of Aloe vera and to evaluate their potential antioxidant, anti-Alzheimer and antidiabetic properties in vitro. The acetone extract, methanolic extract and its four fractions were subjected to quantitative determination of polyphenol, flavonoid, flavonol, condensed tannin and hydrolysable tannin contents. Then, the antioxidant properties of all extracts and fractions were evaluated by using DPPH free radical scavenging, ABTS cation radical decolorization, Cupric Reducing Antioxidant Capacity and Metal Chelating Activity assays. Anti-Alzheimer activity was tested against acetylcholinesterase and butyrylcholinesterase enzymes using the Ellman method. The antidiabetic activity was evaluated by using Alpha-Glucosidase Inhibition Assay. The acetone extract showed the highest amount of total phenolic content (TPC), total flavonoid content (TFC), flavonol content (FLC) and total condensed tannins content (TCTC) compared to others extracts and fractions. The water fraction exhibited the low content of TFC, TPC and FLC. The content of total hydrolysable tannins (THTC) varied from 0.94 ± 0.01 to 1.22 ± 0.02 µg TAE/mg extract. The methanolic extract exhibited highest antioxidant activity in all tests (IC50 value: 24.21±0.30 μg/mL in DPPH assay, IC50 value : 30.75±1.67 μg/mL in ABTS assay, IC50 value: 140.99±2.95 µg/mL in metal chelating activity, and

Ultrasound-Assisted Extraction, LC–MS/MS Analysis, Anticholinesterase, and Antioxidant Activities of Valuable Natural Metabolites from Astragalus armatus Willd.: In Silico Molecular Docking and In Vitro Enzymatic Studies

“…These findings can be explained by the existence of chemicals in the extracts that are responsible for the inhibition of the AChE [ 4 ] and particularly by the ethanolic extract’s flavonoids. Our results agree with those of the investigations conducted by Teyeb et al [ 35 ].…”

“…These biomolecules have significant importance in reducing oxidative stress, which could destroy the biological mechanisms in the human body [ 3 ]. In reality, the preference for natural substances is also a consequence of the lack of adverse effects where numerous studies have shown that natural compounds counteract the negative effects of pharmaceutical treatments, implying that these natural molecules have greater medicinal value [ 4 ].…”

A Wonder Plant Aloe vera L. (Liliaceae): An Overview of its Folk Traditional Uses, Phytoconstituents, Biological Activities, and Cosmaceutical Applications

“…The chemical makeup of Aloe varies by species, climate, and growth conditions and some important phytochemicals reported in Aloe include alkaloids, flavonoids, saponins, terpenoids, glycosides, tannins, phenolic compounds, carbohydrates, protein, sterols, protein, tri-terpenoids, glucose, and galactose, etc. and some derivatives [17,[25][26][27]. Vitamins, enzymes, micro and macro minerals, sugars, anthraquinones, campesterol, sitosterol and lupeol, salicylic acid, and amino acids were also reported [28].…”

“…A lot of studies have reported diverse classes of phytochemicals (Table 2) in Aloe species using different methods [17,[25][26][27][51][52][53][54][55][56][57][58]. Sathyaprabha et al [51] found tannins, saponins, terpenoids, flavonoids, steroids, cardiac glycosides, phlobatannins, squalene oleic acid and dodecanoic acid in A. vera.…”

Chemical Constituents Antioxidant and Antibacterial Activities of the Leaves and Flowers from Gardenia carinata Wallich

“…Phenolic compounds are a main class of secondary metabolites in medicinal plants. The beneficial effects of phenolic compounds is attributed to the antioxidant activity that mainly due to the capacity of scavenging free radicals, donating hydrogen atoms, electrons, or chelate metal cations [Bendjedid, et al, 2020]. Gardenia carinata Wallich., belonging to the family Rubiaceae, is native from Malaysia and Indonesia and it has been found in the southern part of Thailand.…”