In vitro bacterial vaginosis biofilm community manipulation using endolysin therapy

Johnston, William; Ware, Alicia; Kuiters, Willemijn Frederique; Delaney, Christopher; Brown, Jason L.; Hagen, Suzanne; Corcoran, David; Cummings, Matthew; Ramage, Gordon; Kean, Ryan

doi:10.1016/j.bioflm.2022.100101

Cited by 8 publications

(4 citation statements)

References 47 publications

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“…Collectively, these data suggest that posttreatment persistence of Gardnerella spp contributes to treatment failure. Metronidazole has limited ability to disrupt established Gardnerella biofilms [ 40 ], and in vitro studies have found that the minimum inhibitory concentration for both metronidazole and clindamycin is higher for biofilm-forming G vaginalis isolates compared to planktonic isolates [ 41 , 42 ]. Furthermore, 2 recent studies both reported that while metronidazole and clindamycin had some in vitro activity against single-species Gardnerella biofilms, neither antibiotic significantly altered the composition of multispecies biofilms [ 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…Metronidazole has limited ability to disrupt established Gardnerella biofilms [ 40 ], and in vitro studies have found that the minimum inhibitory concentration for both metronidazole and clindamycin is higher for biofilm-forming G vaginalis isolates compared to planktonic isolates [ 41 , 42 ]. Furthermore, 2 recent studies both reported that while metronidazole and clindamycin had some in vitro activity against single-species Gardnerella biofilms, neither antibiotic significantly altered the composition of multispecies biofilms [ 42 , 43 ]. Together these data suggest that antibiofilm agents may be needed to effectively treat BV [ 30 ], particularly in the setting of persistent infections.…”

Section: Discussionmentioning

confidence: 99%

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Prevotella and Gardnerella Are Associated With Treatment Failure Following First-line Antibiotics for Bacterial Vaginosis

Plummer,

Sfameni,

Vodstrcil

et al. 2023

The Journal of Infectious Diseases

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Background Bacterial vaginosis (BV) is a common vaginal dysbiosis that often recurs following first-line antibiotics. We investigated if vaginal microbiota composition was associated with BV recurrence. Methods We analysed samples and data from 121 women who participated in three published trials evaluating novel interventions for improving BV cure, including concurrent antibiotic treatment of regular sexual partners (RSP). Women diagnosed with BV received first-line antibiotics and self-collected vaginal swabs pre-treatment and the day after finishing antibiotics (immediately post-treatment). 16S rRNA gene sequencing was performed on vaginal samples. Logistic regression explored associations between BV recurrence and features of the vaginal microbiota pre- and post-treatment. Results Sixteen women (13%; 95%CI:8-21%) experienced BV recurrence within one-month of treatment. Women with an untreated RSP were more likely to experience recurrence than women with no RSP (P=0.008) or an RSP who received treatment (P=0.011). A higher abundance of Prevotella pre-treatment (adjusted odds ratio [AOR]=1.35, 95%CI:1.05,1.91) and Gardnerella immediately post-treatment (AOR=1.23, 95%CI:1.03,1.49) were associated with increased odds of BV recurrence. Conclusions Having specific Prevotella species prior to recommended treatment and persistence of Gardnerella immediately post-treatment may contribute to the high rates of BV recurrence. Interventions that target these taxa are likely required to achieve sustained BV cure.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prevotella and Gardnerella Are Associated With Treatment Failure Following First-line Antibiotics for Bacterial Vaginosis

Plummer,

Sfameni,

Vodstrcil

et al. 2023

The Journal of Infectious Diseases

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“…The phage endolysin had high anti-G. vaginalis and slightly anti-P. bivia but no anti-F. vaginae activity. Johnston et al (2023) also investigated how endolysin therapy against G. vaginalis biofilm works in vitro. In their study, a four-species biofilm made of G. vaginalis, F. vaginae, P. bivia, and Mobiluncus curtisii was treated using an anti-Gardnerella endolysin (CCB7.1) as this species is the most abundant in polymicrobial community.…”

Section: Mixed Therapies Based On Phages or Phage-delivered Enzymesmentioning

confidence: 99%

Advances in bacteriophage-mediated strategies for combating polymicrobial biofilms

Gliźniewicz,

Miłek,

Olszewska

et al. 2024

Front. Microbiol.

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Bacteria and fungi tend to coexist within biofilms instead of in planktonic states. Usually, such communities include cross-kingdom microorganisms, which make them harder to remove from abiotic surfaces or infection sites. Additionally, the produced biofilm matrix protects embedded microorganisms from antibiotics, disinfectants, or the host immune system. Therefore, classic therapies based on antibiotics might be ineffective, especially when multidrug-resistant bacteria are causative factors. The complexities surrounding the eradication of biofilms from diverse surfaces and the human body have spurred the exploration of alternative therapeutic modalities. Among these options, bacteriophages and their enzymatic counterparts have emerged as promising candidates, either employed independently or in synergy with antibiotics and other agents. Phages are natural bacteria killers because of mechanisms of action that differ from antibiotics, phages might answer worldwide problems with bacterial infections. In this review, we report the attempts to use bacteriophages in combating polymicrobial biofilms in in vitro studies, using different models, including the therapeutical use of phages. In addition, we sum up the advantages, disadvantages, and perspectives of phage therapy.

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“…Simultaneously, at parallel session 10, chaired by Niamh Harrington (University of Warwick, UK) and Estrella Rojo-Molinero (LOC, HUSE, Palma, Spain), the issue “Cooperation, competition or quite the opposite: The complex interactions in biofilm communities” was addressed. On this topic, Johnston et al [ 21 ] presented a poster at the EBF 2022 and wrote a paper about the efficacy of the Gardnerella -specific endolysin CCB7.1 against G. vaginalis in polymicrobial biofilms altering the overall community dynamic and composition.…”

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confidence: 99%

Eurobiofilms 2022: A translational perspective of biofilm-related persistent infections

Macià,

Borghi,

Oliver

2024

Biofilm

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In vitro bacterial vaginosis biofilm community manipulation using endolysin therapy

Cited by 8 publications

References 47 publications

Prevotella and Gardnerella Are Associated With Treatment Failure Following First-line Antibiotics for Bacterial Vaginosis

Prevotella and Gardnerella Are Associated With Treatment Failure Following First-line Antibiotics for Bacterial Vaginosis

Advances in bacteriophage-mediated strategies for combating polymicrobial biofilms

Eurobiofilms 2022: A translational perspective of biofilm-related persistent infections

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