In Vitro Behavior of Primary Human Osteoblasts Onto Microrough Titanium Surface

Abstract

Results indicate that the test group surface is more biocompatible, well tolerated, and suitable for supporting osteoblasts growth and proliferation.

Osteoblastic differentiating potential of dental pulp stem cells <i>in vitro</i> cultured on a chemically modified microrough titanium surface

“…For this reason the rough sandblasted surface was treated by organic or inorganic acid etching which smoothened the peaks created by the formation of microcavities, and promoted protein adhesion which is essential for the early stages of bone healing 30) . It was already reported, in a previous study from our laboratory, performed on the same titanium discs, that the inorganic ions surface coating after acid etching surface was found to be more biocompatible and better tolerated by human osteoblasts 9) . A morphological study of the titanium discs, performed by AFM, reported in our previous work 9) , evidences a nanometric modification of the surface.…”

“…It was already reported, in a previous study from our laboratory, performed on the same titanium discs, that the inorganic ions surface coating after acid etching surface was found to be more biocompatible and better tolerated by human osteoblasts 9) . A morphological study of the titanium discs, performed by AFM, reported in our previous work 9) , evidences a nanometric modification of the surface. The innovation of this surface is represented by the surface treatment which create a stable layer on titanium discs able to better promote cell adhesion, as firstly demonstrated by Zizzari et al 9) ,and subsequently by Gehrke et al 24) .…”

“…A morphological study of the titanium discs, performed by AFM, reported in our previous work 9) , evidences a nanometric modification of the surface. The innovation of this surface is represented by the surface treatment which create a stable layer on titanium discs able to better promote cell adhesion, as firstly demonstrated by Zizzari et al 9) ,and subsequently by Gehrke et al 24) . In the present work, the goal was to better understand if this surface treatment could also have a positive influence on the differentiation of mesenchymal stem cells towards the osteoblastic lineage.…”

“…Firstly, surface treatment with inorganic ions induces a significant roughness modification which is responsible, in turn, of an increased cell adhesion and of a reduced osteoblasts cytotoxicity, as demonstrated in our previous paper 9) . Furthermore, it can be argued that magnesium and calcium addition on test surface plays a pivotal role in promoting and enhancing cell differentiation thus improving bone-implant contact, as supported by the scientific literature 21,22,24) .…”

“…To improve the osteoconductive and osteogenic properties for optimal osteointegration, the surface of titanium implants was constantly modified, mostly trying to increase its hydrophilicity 7) . The microstructure and the nanostructure of the titanium surface influenced the behavior of mesenchymal precursors of osteoblasts, thus affecting cell proliferation and differentiation towards the osteoblastic lineage [8][9][10][11] . Mesenchymal stem cells are capable of self-renewal and, when appropriately induced, of differentiating into many cellular lineages [12][13][14] , undifferentiated and highly clonogenic cells may be isolated from the dental pulp, the periodontal ligament and the dental papilla 15,16) .…”