In Vitro Binding Assay of 31Methionine-oxidized Cholecystokinin Octapeptide to the CCKB Receptor

A novel flow-injection analysis system for evaluation of antioxidants by using sodium dichloroisocyanurate as a source of hypochlorite anion

“…Recently, we reported that hydroxyl radicals could oxidize specifically 27 Met of glucagon (6), and furthermore that the ability of 27 Met sulfoxide of glucagon to produce cAMP was reduced to approximately 25% of that of native glucagon in HepG2 cells (6). A similar phenomenon has been observed in the case of cholecystokinin octapeptide (CCK8) (7), in which the oxidized CCK8 revealed in vitro-altered bioactivity in the LOVO cells (8). Similarly, oxidation of functionalized proteins or bioactive peptides by ROS could deteriorate their original and regular functions (9,10).…”