In vitro Biocompatibility Evaluation of Anodic Alumina Substrates with Electrochemically Embedded Silver

Kiradzhiyska, Denitsa; Batsalova, Tsvetelina; Dzhambazov, Balik; Mancheva, Rositsa

doi:10.37358/rc.20.10.8352

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…Figure 5 represents the obtained results. In accordance with our previous findings [27] NIH/3T3 cells demonstrated higher sensitivity to anodic alumina and silver-modified alumina substrates compared to human fibroblasts. The two specimens with anodized for 30 or 60 min alumina film showed the lowest cell survival level.…”

Section: Biocompatibility Of Silver-modified Alumina Substratessupporting

confidence: 93%

“…It was also found that the concentration of Ag is higher at the anodic films formed in the acid electrolyte for 60 min. It is considered that cell growth, cytotoxicity, genotoxicity and biocompatibility depend on the morphological characteristics such as size, shape, surface charge, concentration, distribution of particles, exposure time and cell type [27,28]. In order to determine the concentration of deposited Ag particles into the anodized alumina layers, all samples were submitted to induction coupled plasma optical emission spectroscopy (ICP-OES) and the acquired quantitative data are graphically represented in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

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Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications

Kiradzhiyska

Milcheva

Mancheva

et al. 2021

Metals

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The present study reports a specific method for preparation of silver-modified anodic alumina substrates intended for biomaterial applications. Al2O3 coatings were obtained by anodization of technically pure aluminum alloy in sulfuric acid electrolyte. Silver deposition into the pores of the anodic structures was carried out employing in situ thermal reduction for different time periods. The obtained coatings were characterized using scanning electron microscopy (SEM), potentiodynamic scanning after 168 h in 3.5% NaCl solution and bioassays with human fibroblast and NIH/3T3 cell lines. The modified alumina substrates demonstrated better biocompatibility compared to the control anodic Al2O3 pads indicated by increased percent cell survival following in vitro culture with human and mouse fibroblasts. The Ag-deposition time did not affect considerably the biocompatibility of the investigated anodic layers. SEM analyses indicated that mouse NIH/3T3 cells and human fibroblasts adhere to the silver-coated alumina substrates retaining normal morphology and ability to form cell monolayer. Therefore, the present studies demonstrate that silver coating of anodic alumina substrates improves their biocompatibility and their eventual biomedical application.

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Section: Biocompatibility Of Silver-modified Alumina Substratessupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications

Kiradzhiyska

Milcheva

Mancheva

et al. 2021

Metals

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Silver-Doped Anodic Alumina with Antimicrobial Properties—Synthesis and Characterization

Kiradzhiyska

Milcheva

Batsalova

et al. 2022

Metals

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The incessant need for the elimination of pathogenic viruses and multi-drug resistant bacteria has been a critical issue during recent decades, and requires the creation of new antimicrobial materials. Our study describes the production of silver-modified anodic alumina substrates by two methods, and estimation of their bactericidal activity. Aluminum oxide coatings were obtained via an anodization process of low-purity aluminum in an acidic bath for different time periods. The realization of silver infiltration into the pores of the alumina layers was carried out employing two different routes—electrochemical deposition, and in situ thermal reduction. The obtained films were characterized using scanning electron microscopy (SEM). Changes in the surface morphology and thickness of the initial oxide structures after hot water sealing procedure were observed. The presence of silver inside the pores of the alumina layers was also assessed. It was found that silver electrodeposition resulted in greater surface saturation. Large silver accumulations were observed on the thinner anodic films which experienced electroplating for longer time periods. Finally, the antibacterial activity of the modified alumina structures against Gram-negative (Escherichia coli) and Gram-positive (Bacillus cereus) bacteria was evaluated. The results demonstrate that silver deposits acquired by the electrochemical technique improve the bactericidal efficiency of the anodic aluminum oxide (AAO) layers. On the contrary, alumina structures with chemically embedded Ag particles did not show significant antibacterial properties. Overall, the present studies demonstrate that biological activity of silver-doped AAO films depends on the techniques used for their modification.

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In vitro Biocompatibility Evaluation of Anodic Alumina Substrates with Electrochemically Embedded Silver

Cited by 2 publications

References 20 publications

Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications

Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications

Silver-Doped Anodic Alumina with Antimicrobial Properties—Synthesis and Characterization

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