In vitro biocompatibility evaluation of a heparinizable material (PUPA), based on polyurethane and poly(amido-amine) components

Albanese, Antonietta; Barbucci, Rolando; Belleville, J; Bowry, Sudhir K; Eloy, R.; Lemke, Horst‐Dieter; Sabatini, Laura

doi:10.1016/0142-9612(94)90262-3

Cited by 49 publications

(20 citation statements)

References 12 publications

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“…The PUPA lms were heparinized as described elsewhere [33]: brie y, they were kept in a 0.5% (w /v) solution of UH (obtained by mixing 0.5 g of UH with 100 ml of H 2 O and adding CH 3 COOH at pH 3.0) at 60 ± C for 24 h. The same procedure was used to immobilize HyalS 3:5 on the PUPA surface; however, the pH of the HyalS 3:5 solution was 6.0 and the temperature was 55 ± C.…”

Section: Immobilization Of Heparin and Hyals 3:5 On The Surface Of Pupamentioning

confidence: 99%

In vitro study of blood-contacting properties and effect on bacterial adhesion of a polymeric surface with immobilized heparin and sulphated hyaluronic acid

Magnani¹,

Barbucci²,

Montanaro³

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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The blood-contacting properties and the effect on bacterial adhesion of a material based on polyurethane and poly(amido-amine) (PUPA), both in its native form and with the anticoagulant molecules heparin or sulphated hyaluronic acid (HyalS3.5) electrostatically bonded to its surface, were evaluated and compared in vitro. The presence of the biological molecules on the surface was revealed by a dye test and ATR/FTIR analysis. Bound heparin was found to maintain its physiological action, in terms of thrombin inactivation, as well as did free heparin. Moreover, it reduced the degree of platelet adhesion. On the contrary, bound HyalS3.5 lost its anticoagulant activity, though it reduced platelet adhesion. The number of platelets on both modified surfaces was low. Their shape distribution, as determined by SEM, did not differ significantly on the two modified surfaces or with respect to the bare PUPA surface. HyalS3.5 and heparin also inhibited adhesion of Staphylococcus epidermidis to the material. A possible relationship between the platelet and bacterial adhesion is ascribed to the mediating role of plasma proteins.

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Section: Immobilization Of Heparin and Hyals 3:5 On The Surface Of Pupamentioning

confidence: 99%

In vitro study of blood-contacting properties and effect on bacterial adhesion of a polymeric surface with immobilized heparin and sulphated hyaluronic acid

Magnani¹,

Barbucci²,

Montanaro³

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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“…Among these, heparin has been particularly investigated to obtain bloodcompatible materials. [6][7][8][9][10][11] Adding to its anticoagulant properties, heparin has also been shown to inhibit arterial SMC proliferation and intimal hyperplasia in vitro and in vivo. 12,13 Most studies dealt with the coagulant pathway, 14,15 and we proposed to take advantage of its antiproliferative property to elaborate a covalent binding technique of heparin on coating protein gels.…”

Section: Introductionmentioning

confidence: 98%

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

Laemmel

Penhoat

Warocquier-Clerout

et al. 1998

J. Biomed. Mater. Res.

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Gelatin or a mixture of albumin and gelatin has been proposed for the coating of vascular grafts according to their surface thrombogenicity and biocompatibility, and the possibility of biodegradation. Heparin treatment of hemocompatible surfaces improved the patency of prostheses. In this study, different amounts of heparin were immobilized on these protein gels using a water-soluble carbodiimide [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide]. The results showed a coupling of heparin with gelatin and/or albumin at the surface of the gels, stable for as long as 1 month. From 0.20 to 3.60 microg x cm(-2), heparin could be immobilized. The antiproliferative activity of immobilized heparin was controlled toward bovine smooth-muscle cells grown on these gels. Cell growth inhibition was dose dependent, but the percentages of inhibition were lower at day 8 than at day 4 at any heparin concentration used under experimental conditions. Referring to heparin in solution, immobilized heparin displayed an antiproliferative activity that improved the potential interest for coating.

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“…Mixed poly(urethane‐PAA) networks, named PUPA, were more extensively studied . These materials were prepared by first treating A‐PAA with excess diisocyanate in chloroform solution and subsequently coupling the resultant isocyanate‐terminated PAA with a commercial polyurethane via the CONH groups of the latter (Scheme ).…”

Section: Synthetic Featuresmentioning

confidence: 99%

Poly(amidoamine)s: Past, present, and perspectives

Ferruti

2013

J. Polym. Sci. Part A: Polym. Chem.

122

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Poly(amidoamine)s (PAAs) are a family of synthetic polymers obtained by stepwise polyaddition of prim-or sec-amines to bisacrylamides. Nearly all conceivable bisacrylamides and prim-or sec-amines can be employed as monomers endowing PAAs of a structural versatility nearly unique among stepwise polyaddition polymers. PAAs are degradable in aqueous media, including physiological fluids. Many of them are remarkably biocompatible notwithstanding their cationic character. PAAs are per se highly functional polymers and, in addition, can be further functionalized giving rise to an endless variety of polymeric structures meeting the requisites for applications in such apparently disparate fields as inorganic water pollutants scavengers, sensors, drug and protein intracellular carriers, transfection promoters, peptidomimetic antiviral and antimalarial agents. In this review, the unique chemistry of PAAs is discussed and a vast library of PAA structures and PAA applications from the beginning to the present days reported.

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In vitro biocompatibility evaluation of a heparinizable material (PUPA), based on polyurethane and poly(amido-amine) components

Cited by 49 publications

References 12 publications

In vitro study of blood-contacting properties and effect on bacterial adhesion of a polymeric surface with immobilized heparin and sulphated hyaluronic acid

In vitro study of blood-contacting properties and effect on bacterial adhesion of a polymeric surface with immobilized heparin and sulphated hyaluronic acid

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

Poly(amidoamine)s: Past, present, and perspectives

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