In vitro biofilm formation and bactericidal activities of methicillin-resistant Staphylococcus aureus clones prevalent in Korea

Cha, Jeong-Ok; Park, Yong-Keun; Lee, Yeong Seon; Chung, Gyung Tae

doi:10.1016/j.diagmicrobio.2010.11.018

Cited by 39 publications

(32 citation statements)

References 32 publications

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“…Nevertheless, microtiter plate-based models similar to the one used here have demonstrated good correlation with respect to biofilm formation with subcutaneous foreign body infections (38), underlining the potential clinical relevance of the model we developed. In addition, our data are consistent with previous studies evaluating these methods for quantifying biofilm formation or drug effects (14,15,17,19,23,37). However, in contrast to previous studies, our approach allows the characterization of antibiotic activity from a pharmacodynamic perspective.…”

Section: Discussionsupporting

confidence: 89%

“…However, in contrast to previous studies, our approach allows the characterization of antibiotic activity from a pharmacodynamic perspective. As such, our data provide additional information compared to the minimal biofilm eradication concentration (MBEC) (34), which is the parameter most commonly used to quantify antibiotic effects on biofilms (9,11,14,15,17,35,36).…”

Section: Discussionmentioning

confidence: 99%

“…These studies typically evaluate a limited set of drugs in parallel (6)(7)(8)(9)(10)(11)(12)(13)(14)(15) and mainly focus on their effect on viability (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Some reports have measured effects on the biofilm matrix, but these are limited to specific antibiotic concentrations (18,19).…”

mentioning

confidence: 99%

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A Combined Pharmacodynamic Quantitative and Qualitative Model Reveals the Potent Activity of Daptomycin and Delafloxacin against Staphylococcus aureus Biofilms

Bauer

Siala

Tulkens

et al. 2013

Antimicrob Agents Chemother

121

105

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Biofilms are associated with persistence of Staphylococcus aureus infections and therapeutic failures. Our aim was to set up a pharmacodynamic model comparing antibiotic activities against biofilms and examining in parallel their effects on viability and biofilm mass. Biofilms of S. aureus ATCC 25923 (methicillin-sensitive S. aureus [MSSA]) or ATCC 33591 (methicillin-resistant S. aureus [MRSA]) were obtained by culture in 96-well plates for 6 h/24 h. Antibiotic activities were assessed after 24/48 h of exposure to concentrations ranging from 0.5 to 512 times the MIC. Biofilm mass and bacterial viability were quantified using crystal violet and the redox indicator resazurin. Biofilms stained with Live/Dead probes were observed by using confocal microscopy. Concentration-effect curves fitted sigmoidal regressions, with a 50% reduction toward both matrix and viability obtained at sub-MIC or low multiples of MICs against young biofilms for all antibiotics tested. Against mature biofilms, maximal efficacies and potencies were reduced, with none of the antibiotics being able to completely destroy the matrix. Delafloxacin and daptomycin were the most potent, reducing viability by more than 50% at clinically achievable concentrations against both strains, as well as reducing biofilm depth, as observed in confocal microscopy. Rifampin, tigecycline, and moxifloxacin were effective against mature MRSA biofilms, while oxacillin demonstrated activity against MSSA. Fusidic acid, vancomycin, and linezolid were less potent overall. Antibiotic activity depends on biofilm maturity and bacterial strain. The pharmacodynamic model developed allows ranking of antibiotics with respect to efficacy and potency at clinically achievable concentrations and highlights the potential utility of daptomycin and delafloxacin for the treatment of biofilm-related infections.S taphylococcus aureus is a major human pathogen, implicated in both hospital-and community-acquired infections. In addition to the increase in antibiotic resistance that often limits therapeutic options, pathogenic bacteria can adapt and survive in specific microenvironments that are also associated with therapeutic failure and recurrence or persistence of infection. Among them, biofilms play a significant role in persistent infections formed on the surface of implanted medical devices and in deep tissues (1-3). Biofilms are complex aggregates of bacteria encased in an extracellular matrix made of polymeric substances like DNA, polysaccharides, teichoic acids, and proteins (4). Biofilms protect bacteria from host defense and antibiotics, allowing them to remain dormant for long periods in the host, and represent a reservoir for resistance development and for bacterial dissemination within the body. Biofilm formation and growth are finely regulated and are accompanied by metabolic changes that could also affect bacterial response to antibiotics (5).Antibiotic activity against staphylococcal biofilms has been studied in a large variety of in vitro or animal models in an attempt ...

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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A Combined Pharmacodynamic Quantitative and Qualitative Model Reveals the Potent Activity of Daptomycin and Delafloxacin against Staphylococcus aureus Biofilms

Bauer

Siala

Tulkens

et al. 2013

Antimicrob Agents Chemother

121

105

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“…Vancomycin concentrations well over 4000 lg/mL are readily obtained in the knee immediately after infusion, and with a daily infusion regimen, this level can be achieved daily for 6 weeks or longer [46]. Moderate concentrations of antibiotics are necessary to prevent biofilm formation [11,12], but once biofilm and persister cells have formed, concentrations of orders of magnitude greater (as high as 4000 lg/ mL) are necessary to eradicate the bacteria within the biofilm [7,8,11,12,17,27,31,45,49,50]. Clearly, some risk of toxicity exists with direct intraarticular infusion of antibiotics, but this method may be the safest way to achieve high antibiotic levels over time [54][55][56] while allowing for the opportunity to decrease the dose, change antibiotics, or stop them altogether in the case of allergic reactions or renal toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…High local antibiotic concentration around the site of an infected THA is a matter of major clinical importance, because bacteria protected by biofilm require concentrations that are orders of magnitude greater than the minimal inhibitory concentration for the planktonic forms of the same bacterium to eliminate resistant organisms that are protected by the glycocalyx [8,11,12,16,32]. Intravenous antibiotics generally do not achieve these levels of concentration in synovial fluid, but instead achieve levels around two to three times the minimal inhibitory concentration (MIC) [46].…”

Section: Introductionmentioning

confidence: 99%

One-stage Revision With Catheter Infusion of Intraarticular Antibiotics Successfully Treats Infected THA

Whiteside

Roy

2017

Clinical Orthopaedics &Amp; Related Research

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Background Two-stage revision surgery for infected total hip arthroplasty (THA) is commonly advocated, but substantial morbidity and expense are associated with this technique. In certain cases of infected THA, treatment with one-stage revision surgery and intraarticular infusion of antibiotics may offer a reasonable alternative with the distinct advantage of providing a means of delivering the drug in high concentrations. Questions/purposes We describe a protocol for intraarticular delivery of antibiotics to the hip through an indwelling catheter combined with one-stage revision surgery and examine (1) the success as judged by eradication of infection at 1 year when treating chronically infected cemented stems; (2) success in treating late-onset acute infections in well-ingrown cementless stems; and (3) what complications were associated with this approach in a small case series. Methods Between

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Design, synthesis, and biological evaluation of pleuromutilin derivatives containing 1,2,4‐triazole linker

Zhou

Wang

et al. 2023

Drug Development Research

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A series of thioether pleuromutilin derivatives containing 1,2,4‐triazole on the side chain of C14 were designed and synthesized. The in vitro antibacterial activities experiments of the synthesized derivatives showed that compounds 72 and 73 displayed superior in vitro antibacterial effect against MRSA minimal inhibitory concentration (MIC = 0.0625 μg/mL) than tiamulin (MIC = 0.5 μg/mL). The results of time‐kill study and postantibiotic effect study indicated that compound 72 could inhibit the growth of MRSA quickly (−2.16 log10 CFU/mL) and showed certain postantibiotic effect (PAE) time (exposure to 2 × MIC and 4 × MIC for 2 h, the PAE was 1.30 and 1.35 h) against MRSA. Furthermore, the binding mode between compound 72 and 50S ribosome of MRSA was explored by molecular docking and five hydrogen bonds were formed between compound 72 and 50S ribosome.

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In vitro biofilm formation and bactericidal activities of methicillin-resistant Staphylococcus aureus clones prevalent in Korea

Cited by 39 publications

References 32 publications

A Combined Pharmacodynamic Quantitative and Qualitative Model Reveals the Potent Activity of Daptomycin and Delafloxacin against Staphylococcus aureus Biofilms

A Combined Pharmacodynamic Quantitative and Qualitative Model Reveals the Potent Activity of Daptomycin and Delafloxacin against Staphylococcus aureus Biofilms

One-stage Revision With Catheter Infusion of Intraarticular Antibiotics Successfully Treats Infected THA

Design, synthesis, and biological evaluation of pleuromutilin derivatives containing 1,2,4‐triazole linker

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