In vitro Biofumigation of Brassica Tissues Against Potato Stem Rot Caused by Sclerotinia sclerotiorum

Ojaghian, Mohammad Reza; Jiang, Heng; Xie, Guan Lin; Cui, Zhou; Zhang, Jing Ze; Li, Bin

doi:10.5423/ppj.2012.28.2.185

Cited by 33 publications

(32 citation statements)

References 17 publications

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“…There are no directly comparable studies but in a study by Bomford (2009), fresh plant material of B. juncea 'Pacific Gold' incorporated into soil failed to reduce carpogenic germination of S. sclerotiorum sclerotia, although they were only exposed to the biofumigant for three days. In contrast, Ojaghian et al (2012) found that growing and incorporating B. juncea into a field as a green manure significantly reduced disease incidence of S. sclerotiorum on potatoes.…”

Section: Discussionmentioning

confidence: 59%

“…B. juncea was found to be the only cruciferous plant tested to affect sclerotial viability of S. sclerotiorum in another study, delaying myceliogenic germination by seven days, although after ten days approximately 80 % of the sclerotia germinated (Smolinska and Horbowicz 1999). However, whilst Rahimi et al (2013) and Ojaghian et al (2012) also found B. juncea varieties to be the most effective in inhibiting mycelial growth, other studies reported B. oleracea var.…”

Section: Introductionmentioning

confidence: 90%

“…They also reported that freezing and thawing plant tissue increased ITC release efficiencies to 26 % The most effective biofumigant plant for suppressing mycelial growth of S. sclerotiorum in this study was B. juncea 'Caliente 99'. Similarly, Ojaghian et al (2012) reported that fresh macerated tissues of B. juncea 'Bresska' were the most effective in reducing mycelial growth of S. sclerotiorum, when compared to B. napus and B. campestris. Also using fresh macerated plant material, Larkin and Griffin (2007) reported that a B. juncea variety inhibited mycelial growth of S. sclerotiorum by 90.2 %, slightly higher than the 85.3 % found in the present study using a rate of 2 g dried plant material.…”

Section: Discussionmentioning

confidence: 99%

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Volatiles from biofumigant plants have a direct effect on carpogenic germination of sclerotia and mycelial growth of Sclerotinia sclerotiorum

Warmington

Clarkson

2015

Plant Soil

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Original citation:Warmington, Rachel and Clarkson, John P. (2015) Volatiles from biofumigant plants have a direct effect on carpogenic germination of sclerotia and mycelial growth of Sclerotinia sclerotiorum. Plant and Soil. doi: 10.1007/s11104-015-2742-8 Permanent WRAP url: http://wrap.warwick.ac.uk/74305 Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-for profit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Publisher's statement:"The final publication is available at Springer via http://dx.doi.org/10.1007/s11104-015-2742-8." A note on versions:The version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP url' above for details on accessing the published version and note that access may require a subscription. AimsSclerotia of Sclerotinia sclerotiorum survive in soil and germinate to produce apothecia which release airborne ascospores. Current control methods rely predominantly on the use of fungicides to kill ascospores. The aim of this research was to identify potential biofumigation treatments which suppress sclerotial germination, providing a potential alternative and long-term approach to disease management. MethodsMicrocosm and in vitro experiments were conducted using dried and milled plant material from six different biofumigant crop plants to determine effects on carpogenic germination of sclerotia and mycelial growth of S. sclerotiorum. ResultsAll biofumigant plants significantly reduced germination of S. sclerotiorum sclerotia in the microcosm experiments, but were less effective against larger sclerotia. In vitro experiments showed a direct effect of biofumigant volatiles on both the mycelial growth of S. sclerotiorum, and carpogenic germination of sclerotia, where the most effective treatment was B. juncea 'Vittasso'.Conclusions It was clear from this study that biofumigant crop plants have potential as part of an integrated disease management system for control of S. sclerotiorum. The microcosm experiments described here provide a straightforward and reliable screening method for evaluating different biofumigants for activity.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Volatiles from biofumigant plants have a direct effect on carpogenic germination of sclerotia and mycelial growth of Sclerotinia sclerotiorum

Warmington

Clarkson

2015

Plant Soil

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“…Studies detected that volatile molecules emitted by fungi cultures had nematicide and nematostatic effects on the Meloidogyne species (Riga et al, 2008;Freire et al, 2012). Other studies have shown nematicide and fungicidal effects of VOCs produced by plants, such as isothiocyanates (Lazzeri et al, 1993;Zasada and Ferris, 2003;Ojaghian et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Research has shown that plants and micro-organisms that can produce volatile organic compounds (VOCs) in soils and substrates may be used to treat diseased plants through biofumigation (Neves et al, 2007;Ojaghian et al, 2012). In addition to toxicity to pathogens, VOCs can attract natural enemies and can act as an activation signal for resistance-related genes (Aharoni et al, 2003;Arimura et al, 2005;Kessler et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Pimenta

Ferreira

Pedroso

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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Since volatiles do not leave residues in food and cause low toxicity to humans, they can act as a skeleton for new nematicidal molecules, once they have demonstrated activity against phytonematodes. The aim of this study was to evaluate the effect of volatile organic compounds (VOCs) released by wood-associated fungi on controling second-stage juveniles (J2) of Meloidogyne incognita. All 28 wood-associated fungi isolates produced VOCs which caused in excess of 76 % immobility in exposed J2 of M. incognita. The fungus isolate VOCs also caused significant mortality compared to control when J2 were exposed to them. After 6 h of exposure, the fungus species Epicoccum nigrum and Schizophyllum commune produced VOCs that immobilized exposed J2 compounds, in relation to the control. When J2 were inoculated into tomato under greenhouse conditions, the M. incognita infectivity and reproduction were reduced by exposure to the VOCs. Volatiles produced by most of the fungi isolates and analyzed by gas chromatograph coupled to a mass spectrometer (CG-MS), included alcohols, esters, terpenes, and ketones. Certain compounds appeared only in S. commune or in E. nigrum suggesting their involvement in the high level of damage caused to nematode reproduction on tomato. Volatiles emitted by wood fungi demonstrated another mode of action of these microorganisms in nature.

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Uncovering the biofumigant capacity of allyl isothiocyanate from several Brassicaceae crops against Fusarium pathogens in maize

et al. 2020

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BACKGROUND: Driven by environmental concerns, chemical fumigants are no longer allowed in many countries. Therefore, other strategies for reducing fungal inoculum in soils and on crop debris are being explored. In the present study, several Brassicaceae crops were screened for their potential to control Fusarium gramineaum and Fusarium poae mycelial growth in an in vitro inverted Petri dish experiment. Volatile production was measured using gas chromatography-mass spectrometry headspace analysis. A selection of cultivars from each crop species was further investigated using a pot experiment with maize. RESULTS: Ethiopian mustard (Brassica carinata) and brown mustard (Brassica juncea) released volatile allyl isothiocyanate (AITC) and a higher concentration of AITC was correlated with a better fungal growth reduction in the in vitro screening. Brown mustard cultivar Etamine completely inhibited growth of both Fusarium spp. Pure AITC in a solution with methanol resulted in a sigmoid dose-response curve for both Fusarium spp. tested. Fusarium poae appeared to be more tolerant to AITC than F. graminearum. A pot experiment revealed that the incorporation of brown mustard plant material could alleviate the clear negative effect of F. graminearum infection on maize growth. CONCLUSION: The present study demonstrated the correlation between the fungistatic effect of biofumigation crops on Fusarium spp. and their production of volatile AITC in vitro, without the addition of exogenous enzymes, and confirmed the biofumigation potential of brown mustard in a pot experiment with maize. These results may help farmers when selecting a green manure crop suitable for biofumigation.

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In vitro Biofumigation of Brassica Tissues Against Potato Stem Rot Caused by Sclerotinia sclerotiorum

Cited by 33 publications

References 17 publications

Volatiles from biofumigant plants have a direct effect on carpogenic germination of sclerotia and mycelial growth of Sclerotinia sclerotiorum

Volatiles from biofumigant plants have a direct effect on carpogenic germination of sclerotia and mycelial growth of Sclerotinia sclerotiorum

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Uncovering the biofumigant capacity of allyl isothiocyanate from several Brassicaceae crops against Fusarium pathogens in maize

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