In vitro biological activities of magainin alone or in combination with nisin

Cruz-Chamorro, Lidia; Puertollano, Marı́a A.; Puertollano, Elena; Cienfuegos, Gerardo Álvarez de; Pablo, Manuel A. de

doi:10.1016/j.peptides.2005.11.008

Cited by 68 publications

(42 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The present result showed that magainin II strongly reduces the viability of colo 320 DM cells. Consistent with these results, previous reports indicated that magainin II inhibits cell proliferation in different cancer cell lines 6,17 .…”

Section: Resultssupporting

confidence: 90%

See 1 more Smart Citation

Untitled

2016

IJPSR

View full text Add to dashboard Cite

Antimicrobial peptides (AMP) showed a broad spectrum of cytotoxic activity against cancer cells, which could provide a new class of anticancer drugs. In this study, we have evaluated the cytotoxic and apoptotic activity of magainin II, an AMP compared with 5-Fluorouracil (5-FU) in colo320 DM cancer cells, and magainin II cytotoxicity was assessed on vero cell line by MTT. The cells cultured in the presence or absence of magainin II and 5-FU. The magainin II inhibited cell viability in dose dependent manner was quantified by MTT assay. The IC 50 of magainin II was found to be 89.3nM and at this concentration the cytotoxicity was not observed in vero cell line. The cytotoxicity of the magainin II was further confirmed by lactate dehydrogenase (LDH) assay and neutral red uptake assay (NRU). Apoptosis induction was verified by DNA fragmentation analysis. The morphological changes in colo 320 DM cells was measured by DAPI and acridine orange/ethidium bromide staining, showed nuclear condensation, and this substantiates the apoptotic action of magainin II. The cell cycle analysis by FACS revealed that there was a accumulation of cells at G0/G1 phase in magainin II treated cells. Overall, these results revealed that magainin II has selective cytotoxicity in cancer cells and has the potential to induce apoptosis. Magainin II may offer a novel and effective therapeutic candidate in the treatment of colon cancer with potentially low cytotoxic effects on normal cells. These findings highlight the importance of magainin II as a drug capable of exerting an in vitro antitumoral activity by triggering apoptosis.

show abstract

Section: Resultssupporting

confidence: 90%

“…Similar results were observed in cecropin treated human promyelocytic leukemia HL-60 cell line 28 . Magainin I or BMAP-28 (bovine cathelicidinderived AMP), cationic peptides which also induced DNA fragmentation 17,29 .…”

Section: Fig 1: Determination Of Cytotoxicity (A) Dose-dependent Cymentioning

confidence: 99%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

show abstract

“…AMPs are effective antibiotic agents commonly found in plants, animals, microorganisms, and viruses (29). Magainins are ␣-helical cationic antimicrobial peptides (CAMPs) first isolated from Xenopus laevis (65), and they have been described as possessing other actions, such as antileishmanial (28) and antitumor (18,38) actions. The modes of action proposed for magainins are based on the model of cell membrane disruption and include the random insertion of peptide hydrophobic regions into the membrane alignment, the removal of membrane sections, and pore formation (32,33).…”

mentioning

confidence: 99%

Deciphering the Magainin Resistance Process of Escherichia coli Strains in Light of the Cytosolic Proteome

Maria-Neto

Cândido

Rodrigues

et al. 2012

Antimicrob Agents Chemother

View full text Add to dashboard Cite

e Antimicrobial peptides (AMPs) are effective antibiotic agents commonly found in plants, animals, and microorganisms, and they have been suggested as the future of antimicrobial chemotherapies. It is vital to understand the molecular details that define the mechanism of action of resistance to AMPs for a rational planning of the next antibiotic generation and also to shed some light on the complex AMP mechanism of action. Here, the antibiotic resistance of Escherichia coli ATCC 8739 to magainin I was evaluated in the cytosolic subproteome. Magainin-resistant strains were selected after 10 subsequent spreads at subinhibitory concentrations of magainin I (37.5 mg · liter ؊1 ), and their cytosolic proteomes were further compared to those of magainin-susceptible strains through two-dimensional electrophoresis analysis. As a result, 41 differentially expressed proteins were detected by in silico analysis and further identified by tandem mass spectrometry de novo sequencing. Functional categorization indicated an intense metabolic response mainly in energy and nitrogen uptake, stress response, amino acid conversion, and cell wall thickness. Indeed, data reported here show that resistance to cationic antimicrobial peptides possesses a greater molecular complexity than previously supposed, resulting in cell commitment to several metabolic pathways.

show abstract

“…Indeed, infection control in patients with malignant tumors is one of the most important aspects of hospital management. Amphibian skin secretions contain a large number of antimicrobial peptides, including aurein 1.2, citropin 1.1, gaegurins, magainin, and magainin analogues, which have shown selective toxicity against human cancer cells [3,4,5]. There are now 105 antitumor peptides listed in the antimicrobial peptide database (APD, http://aps.unmc.edu/AP), 11 of which were isolated by Roaek [6] from the Australian bull frogs.…”

Section: Introductionmentioning

confidence: 99%