In Vitro Biological Characterization of Silver-Doped Anodic Oxide Coating on Titanium

Oleshko, Oleksandr; Liubchak, Iryna; Husak, Yevheniia; Korniienko, Viktoriia; Yusupova, A. A.; Oleshko, Tetiana; Banasiuk, Rafał; Szkodo, Marek; Matros-Taranets, Igor; Kazek-Kęsik, Alicja; Simka, Wojciech; Pogorielov, Мaksym

doi:10.3390/ma13194359

Cited by 21 publications

(11 citation statements)

References 49 publications

(54 reference statements)

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“…The Cur-AgNPs also showed low cytotoxicity. These results stand alongside previous work suggesting that AgNPs mediate stimulation of transcriptional changes towards improved skin appearance 40 , 41 . This reflects the function of fibroblasts in mediating the secretion of extracellular matrix facilitating collagen production which corresponds to the re-epithelialization phase of wound healing 42 .…”

Section: Resultssupporting

confidence: 88%

Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels

Bhubhanil

Talodthaisong

Khongkow

et al. 2021

Sci Rep

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Biocompatible materials that act as scaffolds for regenerative medicine are of enormous interest. Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce. Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties. We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts. We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels. Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways. As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.

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Section: Resultssupporting

confidence: 88%

Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels

Bhubhanil

Talodthaisong

Khongkow

et al. 2021

Sci Rep

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“…All samples were rinsed with distilled water and ultrasonically cleaned in deionized water and 2-propanol for 5 minutes prior to PEO treatment. As the electrolyte, a suspension of Ag nanoparticles (180 mg L -1 ) in a solution containing an organic chelating agent (nitrilotriacetic acid), KH2PO4, and Ca(OH)2 was used [51].As the cathode, a titanium mesh was used, and as the anode, the treated scaffold sample was used. To remove any unattached AgNPs, the treated samples were ultrasonically cleaned after the PEO process in 2-propanol for 5 minutes and rinsed with deionized water.…”

Section: Plasma Electrolytic Oxidation (Peo) Of Scaffoldsmentioning

confidence: 99%

“…Ag nanoparticles were obtained from Nanopure, Poland. The characterization of the AgNPs was described in [51].…”

Section: Plasma Electrolytic Oxidation (Peo) Of Scaffoldsmentioning

confidence: 99%

In vitro evaluation of electrochemically bioactivated Ti6Al4V 3D porous scaffolds

Myakinin

Turlybekuly

Pogrebnjak

et al. 2021

Materials Science and Engineering: C

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Triply periodic minimal surfaces (TPMS) are known for their advanced mechanical properties and are wrinkle-free with a smooth local topology. These surfaces provide suitable conditions for cell attachment and proliferation. In this study, the in vitro osteoinductive and antibacterial properties of scaffolds with different minimal pore diameters and architectures were investigated. For the first time, scaffolds with TPMS architecture were treated electrochemically by plasma electrolytic oxidation (PEO) with and without silver nanoparticles (AgNPs) to enhance the surface bioactivity. It was found that the scaffold architecture had a greater impact on the osteoblast cell activity than the pore size. Through control of the architecture type, the collagen production by osteoblast cells increased by 18.9% and by 43.0% in the case of additional surface PEO bioactivation. The manufactured scaffolds demonstrated an extremely low quasi-elastic modulus (comparable with trabecular and cortical bone), which was 5 -10 times lower than that of bulk titanium (6.4-11.4 GPa vs 100-105 GPa). The AgNPs provided antibacterial properties against both gram-positive and gram-negative bacteria and had no significant impact on the osteoblast cell growth. Complex experimental results show the in vitro effectiveness of the PEO-modified TPMS architecture, which could positively impact the clinical applications of porous bioactive implants.

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“…These dents can be flexible, giving ChAs 06038-2 highly reactive and versatile binding possibilities not only to form complexes with metal ions, but also to bind with metal oxide surface [18]. We recently showed that the NTA can improve deposition of Ag nanoparticles into the PEO surface layers on titanium implants to render them the bacteriostatic properties [10]. Here we continued to investigate the positive effects of addition of the NTA into the PEO bath electrolyte containing potassium phosphate and calcium formate.…”

Section: Introductionmentioning

confidence: 99%

“…The PEO surfaces were made suitable for successful bone integration [6] and to support proliferation of mesenchymal stem cells [7]. On the other hand, the PEO layers were rendered bacteriostatic properties via incorporation of Ag and Cu nanoparticles [8,9,10]. The surface morphology of the PEO layers can also be modified in order to achieve the desired structure which could improve cellular adhesion and viability [11].…”

Section: Introductionmentioning

confidence: 99%

A New Solution for an Electrochemical Modification of Titanium Surface

Kyrylenko¹,

Oleshko²,

Warchoł³

et al. 2020

J. Nano- Electron. Phys.

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Dental/osseous implants manufactured of titanium (Ti) have become a routine and affordable method in medical practice. To increase the overall safety and longtime stability of the implants further, the sophisticated approaches have been investigated in order to supply the implants with the bioactive surface layers. They have to serve two purposes: to increase the osseointegration capacity of the implants and to reduce the chances of bacterial growth and formation of bacterial biofilms leading to periimplantitis. Plasma electrolytic oxidation (PEO) is becoming a promising method to introduce functionalized surface layers on a metal substrate. Various PEO protocols have been suggested in order to achieve better biocompatibility of the dental implants and to increase their resistance to bacterial infections. It was also suggested that the PEO layers could increase resistance to corrosion on the surfaces of metallic implants. The dynamic processes running on the surface of Ti during the PEO processing still require efforts to fully understand the molecular mechanisms of the formation of hard and porous oxide surface layers. We and others have already shown that addition of a chelating agent to the bath electrolyte leads to better outcomes in the morphology and functional characteristics of the implants. Here we report in depth characterization of the PEO parameters and the produced PEO surface layers using the bath electrolyte containing another widely used chelating agent, nitrilotriacetic acid (NTA) along with potassium phosphate and calcium formate. The results will contribute to further understanding the mechanisms of the PEO process and to establishing routine protocols for commercial exploitation of the PEO method.

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In Vitro Biological Characterization of Silver-Doped Anodic Oxide Coating on Titanium

Cited by 21 publications

References 49 publications

Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels

Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels

In vitro evaluation of electrochemically bioactivated Ti6Al4V 3D porous scaffolds

A New Solution for an Electrochemical Modification of Titanium Surface

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