In vitro Biological Evaluation of Small Intestinal Submucosa - Chitosan / Hyaluronic Acid Tridimensional Scaffolds as Deep Wound Healing Dressings

Escobar, Juliana Jaramillo; Nino, Vivian Andrea Talero; Bonilla, Laura Lorena Cardenas; Quintero, Mateo Pineda; Diaz, Julieth Paola Oliveros; Camargo, Carolina Muñoz; Briceño, Juan Carlos

doi:10.1109/sib.2018.8467734

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…Finally, formazan crystals were dissolved by agitation for 5 min and then, each well was analyzed in a BioRad micro plate reader at 595 nm, with a reference wavelength of 655 nm. The results were expressed as the percentage of living cells as calculated from MTT reduction, assuming the absorbance of control cells as 100%, and optical micrographs were taken in order to corroborate the results obtained in the cell viability assays [ 27 , 28 ].…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and Biocompatible Polycaprolactone and Copper Oxide Nanoparticle Wound Dressings against Methicillin-Resistant Staphylococcus aureus

2020

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One of the major health problems linked to methicillin-resistant Staphylococcus aureus (MRSA) is severe diabetic foot ulcers (DFU), which are associated with hospital-acquired infections, lower limb amputations and emerging resistance to the current antibiotics. As an alternative, this work aims to develop a biodegradable and biocompatible material with antimicrobial capacity to prevent DFU. This was achieved by producing active polymeric films with metallic nanoparticles dispersed through a polycaprolactone (PCL) dressing. First, the antimicrobial activity of copper oxide nanoparticles (CuONPs) was tested by the microdilution method, selecting the lowest concentration that has an inhibitory effect on MRSA. Then, active PCL films were prepared and characterized in terms of their physicochemical properties, antimicrobial performance, cytotoxicity, genotoxicity and hemocompatibility. Active films had chemical and thermal properties like the ones without the antimicrobial agents, which was confirmed through FTIR, Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) analysis. In relation to antimicrobial activity, active PCL films inhibited MRSA growth when treated with CuONPs at a concentration of 0.07% (w/w). After exposure to the active film extracts, human foreskin fibroblast cells (ATCC® SCRC1041™) (HFF-1) exhibited a cell viability average above 80% for all treatments and no DNA damage was found. Finally, PCL films with 0.07% (w/w) CuONPs proved to be hemocompatible, and none of the films evaluated had red blood cell breakage greater than 5%, being within the acceptable limits established by the International Organization for Standardization ISO 10993-4:2002.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial and Biocompatible Polycaprolactone and Copper Oxide Nanoparticle Wound Dressings against Methicillin-Resistant Staphylococcus aureus

2020

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“…Membranes composed of chitosan and HA were examined and characterized by numerous investigators [ 94 , 95 , 96 , 97 , 98 , 99 , 100 ]. There are several papers which reported the use of membranes or scaffolds composed of chitosan and HA in the presence of another component, such as gelatin [ 101 , 102 ], alginate [ 103 ], collagen I [ 104 ], arginine derivatives [ 105 ], or polycaprolactone [ 106 ].…”

Section: Introductionmentioning

confidence: 99%

Versatile Use of Chitosan and Hyaluronan in Medicine

Valachová

Šoltés

2021

Molecules

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Chitosan is industrially acquired by the alkaline N-deacetylation of chitin. Chitin belongs to the β-N-acetyl-glucosamine polymers, providing structure, contrary to α-polymers, which supply food with energy. Another β-polymer providing structure is hyaluronan. A lot of studies have been performed on chitosan to explore its industrial use. Since chitosan is biodegradable, non-toxic, bacteriostatic, and fungistatic, it has numerous applications in medicine. Hyaluronan, one of the major structural components of the extracellular matrix in vertebrate tissues, is broadly exploited in medicine as well. This review summarizes the main areas where these two biopolymers have an impact. The reviewed areas mostly cover most medical applications, along with non-medical applications, such as cosmetics.

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Clustering-Based Water Quality Exploration and Visualization in Municipal Water Supply

Shakhari,

Banerjee

2024

2024 IEEE International Conference on Interdisciplinary Approaches in Technology and Management for Social Innovation (IATMSI)

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In vitro Biological Evaluation of Small Intestinal Submucosa - Chitosan / Hyaluronic Acid Tridimensional Scaffolds as Deep Wound Healing Dressings

Cited by 4 publications

References 27 publications

Antimicrobial and Biocompatible Polycaprolactone and Copper Oxide Nanoparticle Wound Dressings against Methicillin-Resistant Staphylococcus aureus

Antimicrobial and Biocompatible Polycaprolactone and Copper Oxide Nanoparticle Wound Dressings against Methicillin-Resistant Staphylococcus aureus

Versatile Use of Chitosan and Hyaluronan in Medicine

Clustering-Based Water Quality Exploration and Visualization in Municipal Water Supply

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