In vitro biotransformation of dimethylarsinic acid and trimethylarsine oxide by anaerobic microflora of mouse cecum analyzed by HPLC-ICP-MS and HPLC-ESI-MS

Kubachka, Kevin M.; Kohan, Michael C.; Conklin, Sean D.; Herbin-Davis, Karen; Creed, John T.; Thomas, David J.

doi:10.1039/b817820h

Cited by 42 publications

(39 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The thiolation of DMAs V and TMAsO by mouse cecal contents was verified in a recent study [32]. One to two minutes after addition of DMAs V , conversion to DMMTAs V and DMDTAs V was detectable; after 24 h, DMAs V was quantitatively converted to DMMTAs V , DMDTAs V as well as TMAsS.…”

Section: Thiolation Of As Compounds By Intestinal Microorganismsmentioning

confidence: 87%

“…As each colon compartment harbors a different microbial community, it can be studied whether metal(loid) metabolism depends on specific fermentation activities and is thus colon region dependent. For example, the thiolation of methylated arsenicals is a process that may depend on the activity of sulfate-reducing microorganisms in the gut [32,41,42]. In addition, Meyer et al previously suggested that metal(loid) methylation maybe a process closely linked to methanogenic activity [85].…”

Section: Recommendations For Future Researchmentioning

confidence: 99%

“…In particular, the thiolation is a highly relevant process, as DMMTAs V is much more efficiently taken up by cells and significantly more toxic in comparison to its oxo-analog, DMAs V [35,36]. From the in vivo studies with rats [22][23][24] as well as the in vitro studies with mice ceca [32] and cultured human colon microbiota [41] it can be inferred that the formation of methylated thioarsenicals can entirely take place in the gut lumen. The large intestine is generally rich in sulfides due to the presence of sulfate reducers.…”

Section: Contribution Of Intestinal As Biotransformation To Overall Mmentioning

confidence: 99%

See 2 more Smart Citations

Biotransformation of metal(loid)s by intestinal microorganisms

Diaz-Bone

Wiele

2010

Pure and Applied Chemistry

View full text Add to dashboard Cite

Many metals and metalloids undergo complex biotransformation processes by micro organisms in the environment, namely, Ge, As, Se, Cd, In, Sn, Sb, Te, Hg, Tl, Pb, Bi, and Po. Though the human intestine harbors a highly diverse and metabolically active microbial community, the knowledge on metal(loid) biotransformation by gut microbiota is limited. Microbial metal(loid) metabolism in the gut is highly relevant when assessing health risks from oral exposure, as both the bioavailability and the toxicity of the ingested compound can be modulated. This review gathers and compares a broad selection of scientific studies on the intestinal biotransformation of metal(loid)s.It can be inferred that metal(loid) biotransformation by intestinal microbiota is a common process, resulting in both beneficial and adverse toxicological effects. Whereas for Hg the intestinal demethylation of methylmercury results in enhanced elimination, highly bioavailable and toxic arsenic and Bi species are formed by intestinal microorganisms. In either case, we conclude that the gut microbial potency should be considered to be taken up in toxicokinetic studies and models for assessing the health risks of oral metal(loid) exposure. This will allow the relevance of intestinal metal(loid) biotransformation to be assessed for human health risks.

show abstract

Section: Thiolation Of As Compounds By Intestinal Microorganismsmentioning

confidence: 87%

Section: Recommendations For Future Researchmentioning

confidence: 99%

Section: Contribution Of Intestinal As Biotransformation To Overall Mmentioning

confidence: 99%

See 1 more Smart Citation

Biotransformation of metal(loid)s by intestinal microorganisms

Diaz-Bone

Wiele

2010

Pure and Applied Chemistry

View full text Add to dashboard Cite

show abstract

“…Nevertheless, the highly toxic MMA III detected as a metabolite by human colon microbiota indicates that As methylation will not lead to detoxification (Van de Wiele et al, 2010). Due to As thiolation by SBR, some thiolated arsenicals, DMMTA V with high toxicity and harmless DMDTA V , are often found in the urine after inorganic As exposure (Kubachka et al, 2009;Naranmandura et al, 2007;Raml et al, 2007).…”

Section: Tablementioning

confidence: 99%

“…Arsenic species that have previously been detected in the colon digests, include arsenate [As(V)], arsenite [As(III)], low-toxicity monomethylarsonic acid (MMA V ) and dimethylarsinic acid (DMA V ), highly toxic monomethylarsonous acid (MMA III ), and monomethylmonothioarsonic acid (MMMTA V ) of unknown toxicity (Van de Wiele et al, 2010). In addition, some studies on urine analysis by As thiolation using animal models revealed sulfur-containing methylated As metabolites, dimethylmonothioarsinic acid (DMMTA V ), and dimethyldithioarsinic acid (DMDTA V ) (Kubachka et al, 2009;Naranmandura et al, 2007;Raml et al, 2007). As bioaccessibility in the gastric and small intestinal phases is used to estimate As potential harm to human health.…”

Section: Introductionmentioning

confidence: 99%

Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME

Yin

Zhang

et al. 2016

Science of The Total Environment

View full text Add to dashboard Cite

• Five in vitro gastrointestinal methods combined with SHIME (colon phase) were used.• As bioaccessibility varied in the colon phase among these methods.• Plenty of As(III) and methylated arsenicals by microbial transformation were observed.• As bioaccessibility in PBET/SBRC-SHIME colon phase were closer to in vivo results for NIST 2710a. Arsenic (As) speciation analysis is essential when evaluating the risks upon oral exposure to As-contaminated soils. In this study, we first investigated the variability in the As bioaccessibility and speciation using a combination of five common in vitro methods (SBRC, PBET, DIN, UBM and IVG) (gastric and small intestinal phases) and the SHIME model (colon phase). Our results indicate that the As bioaccessibility varies in the colon phase. An increase in the As bioaccessibility for SBRC and PBET, and a decrease for UBM and IVG were observed in the colon phase. In addition, we found different extents of methylation and large amounts of arsenite [As(III)] due to microbial reduction in the colon digests. The UBM-SHIME method displayed a higher methylation percentage of 13.5-82.1%, but a lower methylation percentage of 0.2-21.8% was observed in the SBRC-SHIME method. Besides, The MMA V levels in the colon digests were positively correlated with those of As(III) and DMA V , so DMA V can be considered an indicator to evaluate the As metabolic speed of in vitro cultured human gut microbiota. Based on the standard reference soil of NIST 2710a, the As bioaccessibility in the colon phase of PBET-SHIME and SBRC-SHIME were the closest to the in vivo results. Combining in vitro methods and SHIME will remarkably affect the accurate assessment of potential risks to human health associated with oral exposure to soil As.

show abstract

The Chemistry and Metabolism of Arsenic

Thomas

2015

Arsenic

View full text Add to dashboard Cite

In vitro biotransformation of dimethylarsinic acid and trimethylarsine oxide by anaerobic microflora of mouse cecum analyzed by HPLC-ICP-MS and HPLC-ESI-MS

Cited by 42 publications

References 40 publications

Biotransformation of metal(loid)s by intestinal microorganisms

Biotransformation of metal(loid)s by intestinal microorganisms

Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME

The Chemistry and Metabolism of Arsenic

Contact Info

Product

Resources

About