In Vitro Biotransformation of Steroid Hormones by Human Semen

Castañeda, Evangelina; Ríos, Esteban P.; Pérez, Ana E.; Lichtenberg, R.; Cordero, Claudio; Iramain, Carlos A.; Pérez‐Palacios, Gregorio

doi:10.1016/s0015-0282(16)40274-8

Cited by 15 publications

(4 citation statements)

References 19 publications

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“…In agreement with previous reports (De Aloysio et al, 1974;Nieschlag et al, 1978;Schoenfeld et al, 1978;Bain, 1979) the mean values for steroids in seminal plasma are generally lower than those in serum. Such a decrease could be attributed to a limitation in their transference from plasma (the selective 'blood-testis barrier' proposed by Setchell, Voglmayr & Waites, 1969) or to a faster metabolism and/or conjugation of steroids by the genital tract, its accessory glands or even the spermatozoa (Castañeda et al, 1974;Purvis et al, 1975b;Purvis & Diczfalusy, 1976).…”

Section: Discusssionmentioning

confidence: 99%

Hormone levels in serum and seminal plasma of men with different types of azoospermia

Diez¹,

Buitrago²,

Corrales³

et al. 1983

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Hormone concentrations in the serum and seminal plasma of 15 normozoospermic, 17 excretory azoospermic and 14 secretory azoospermic men were measured. The results indicate that: (a) serum FSH and LH levels are markedly elevated in secretory azoospermia, as compared with excretory azoospermia and normozoospermia; (b) serum 17 alpha-hydroxyprogesterone levels are somewhat raised in secretory azoospermia as compared with excretory azoospermia and normozoospermia; (c) serum testosterone levels are lower in both types of azoospermia with respect to normozoospermia; (d) in secretory azoospermia the oestradiol serum levels are relatively high and dihydrotestosterone serum levels relatively low, whereas the serum levels of these hormones in excretory azoospermia are similar to those in normozoospermic men; (e) in the seminal plasma of azoospermic patients the levels of prolactin, progesterone, testosterone, dihydrotestosterone and oestradiol were depressed, but only dihydrotestosterone levels could be of value in differentiating types of azoospermia because they are lower in secretory azoospermia. We suggest that the measurement of FSH, LH, 17 alpha-hydroxyprogesterone, dihydrotestosterone and oestradiol in serum and dihydrotestosterone in seminal plasma may be used in the differential diagnosis between secretory and excretory azoospermia when invasive tests are unavailable.

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Section: Discusssionmentioning

confidence: 99%

Hormone levels in serum and seminal plasma of men with different types of azoospermia

Diez¹,

Buitrago²,

Corrales³

et al. 1983

Reproduction

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“…Human gametes in vitro possess the enzymatic ability to perform several steroidal transformations, including pregnenolone to dehydroepiandrosterone [2], progesterone to androgens [ 14, 151, dehydroepiandrosterone to A4-androstenedione and testosterone [6, 1 11, testosterone to 5a-dihydrotestosterone [ 131, and estradiol-17P to estrone [12]. These conversions suggest that human spermatozoa in vivo might contain C,,, C,,, and C,, steroids, most likely to be of physiological importance in male reproductive processes.…”

Section: Introductionmentioning

confidence: 99%

“…[1,2,6,7,16, 17-3H]progesterone (sp act 100-130 Ci/mmol), [ l ,2,6,7-3H]testosterone (sp act 80-105 Ci/mmol), and [2,4,6,7,16,17-3H]estradiol-17/3 (sp act 140-170 Ci/mmol) were purchased from Amersham International plc, UK. The unlabeled steroids were from Sigma Chemical Co., St. Louis, MO, USA.…”

mentioning

confidence: 99%

Concentrations of Intracellular Sex Steroids in Human Spermatozoa

Chew

Loganath

Peh

et al. 1993

Archives of Andrology

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In an attempt to determine whether human spermatozoa contain steroid hormones, the immunoreactive levels of progesterone, testosterone, and estradiol-17 beta were measured in the extracts of sonicated specimens of sperm obtained as ejaculates from husbands of infertile couples (n = 67, age 26-44 years). In the range of sperm concentration between 3.0 and 869.6 million per ejaculate (143.2 +/- 13.6 mean +/- SEM), the concentrations were as follows: progesterone 127.3-6685.4 fmol/ml (1434.5 +/- 178.6), testosterone 98.3-2219.1 fmol/ml (611.6 +/- 49.0), and estradiol-17 beta 33.0-678.1 fmol/ml (206.9 +/- 19.7). Using Pearson's correlation coefficient, the steroid hormone levels correlated significantly with the total sperm number (p < .02). The spermatozoa contained small but measurable amounts of the sex steroids.

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“…Human spermatozoa are highly specialized cells that contain steroid metabolizing enzymes capable of catalyzing the transformation of pregnenolone to dehydroepiandrosterone [2], progesterone to androgens [ 161, testosterone to A4-androstenedione and 5a-dihydrotestosterone [14], and estradiol-17P to estrone [15]. Based on the validity of these conversions, we have recently identified levels of progesterone, testosterone, estradiol-l7P, 17a-hydroxyprogesterone, and cortisol in human spermatozoa [4, 121. Results from these experiments raised the possibility that these unicellular organisms with high metabolic activity contain key enzymes considered to be exclusive to the steroidogenic pathway operating in placenta, as well as the adrenal glands and gonads.…”

mentioning

confidence: 99%

Characterization of a Δ⁵-3β-Hydroxy Pathway of Testosterone Synthesis in the Human Spermatozoa

Gunasegaram

Loganath

Peh

et al. 1997

Archives of Andrology

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In Vitro Biotransformation of Steroid Hormones by Human Semen

Cited by 15 publications

References 19 publications

Hormone levels in serum and seminal plasma of men with different types of azoospermia

Hormone levels in serum and seminal plasma of men with different types of azoospermia

Concentrations of Intracellular Sex Steroids in Human Spermatozoa

Characterization of a Δ⁵-3β-Hydroxy Pathway of Testosterone Synthesis in the Human Spermatozoa

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In Vitro Biotransformation of Steroid Hormones by Human Semen

Cited by 15 publications

References 19 publications

Hormone levels in serum and seminal plasma of men with different types of azoospermia

Hormone levels in serum and seminal plasma of men with different types of azoospermia

Concentrations of Intracellular Sex Steroids in Human Spermatozoa

Characterization of a Δ5-3β-Hydroxy Pathway of Testosterone Synthesis in the Human Spermatozoa

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Characterization of a Δ⁵-3β-Hydroxy Pathway of Testosterone Synthesis in the Human Spermatozoa