In vitro breeding: application of embryonic stem cells to animal production†

Goszczynski, Daniel Estanislao; Cheng, Hao; Demyda-Peyrás, S.; Medrano, Juan F.; Wu, Jun; Ross, Pablo J.

doi:10.1093/biolre/ioy256

Cited by 45 publications

(30 citation statements)

References 114 publications

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“…Moreover, transfer of IVP embryos is growing worldwide while that of MOET embryos is relatively stable ( Hansen, 2020 ). If procedures to generate gametes from embryonic stem cells become a reality ( Goszczynski et al, 2019 ), the use of IVP embryos will accelerate.…”

Section: Some Ways Forwardmentioning

confidence: 99%

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Hansen

2020

Journal of Animal Science

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Typically, bovine embryos are transferred into recipient females about day 7 after estrus or anticipated ovulation, when the embryo has reached the blastocyst stage of development. All the biological and technical causes for failure of a female to produce a blastocyst 7 d after natural or artificial insemination (AI) are avoided when a blastocyst-stage embryo is transferred into the female. It is reasonable to expect, therefore, that pregnancy success would be higher for embryo transfer (ET) recipients than for inseminated females. This expectation is not usually met unless the recipient is exposed to heat stress or is classified as a repeat-breeder female. Rather, pregnancy success is generally similar for ET and AI. The implication is that either one or more of the technical aspects of ET have not yet been optimized or that underlying female fertility that causes an embryo to die before day 7 also causes it to die later in pregnancy. Improvements in pregnancy success after ET will depend upon making a better embryo, improving uterine receptivity, and forging new tools for production and transfer of embryos. Key to accelerating progress in improving pregnancy rates will be the identification of phenotypes or phenomes that allow the prediction of embryo competence for survival and maternal capacity to support embryonic development.

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Section: Some Ways Forwardmentioning

confidence: 99%

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Hansen

2020

Journal of Animal Science

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“…New advances in in vitro germ cell and gamete development from mouse ESCs have led to recent interest in the potential for in vitro breeding in livestock (Goszczynski et al, 2018;Hou et al, 2018). The advantage of this proposed method would be that it could effectively remove the wait required for animals to reach sexual maturity prior to meiosis and conception.…”

Section: Generation Intervalmentioning

confidence: 99%

“…In vitro breeding could potentially reduce the generation time for cattle 10-fold from approximately 2.5 to 0.25 years (Goszczynski et al, 2018). The potential increase in the rate of genetic gain is not limited to the consequences of a reduced generation time; in vitro breeding could also allow thousands of embryos to be produced from a single mating, thereby increasing the intensity of selection (i) and the chance of obtaining elite individuals from each parental pairing.…”

Section: Generation Intervalmentioning

confidence: 99%

Genome editing approaches to augment livestock breeding programs

Bishop

Eenennaam

2020

Journal of Experimental Biology

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The prospect of genome editing offers a number of promising opportunities for livestock breeders. Firstly, these tools can be used in functional genomics to elucidate gene function, and identify causal variants underlying monogenic traits. Secondly, they can be used to precisely introduce useful genetic variation into structured livestock breeding programs. Such variation may include repair of genetic defects, the inactivation of undesired genes, and the moving of useful alleles and haplotypes between breeds in the absence of linkage drag. Editing could also be used to accelerate the rate of genetic progress by enabling the replacement of the germ cell lineage of commercial breeding animals with cells derived from genetically elite lines. In the future, editing may also provide a useful complement to evolving approaches to decrease the length of the generation interval through in vitro generation of gametes. For editing to be adopted, it will need to seamlessly integrate with livestock breeding schemes. This will likely involve introducing edits into multiple elite animals to avoid genetic bottlenecks. It will also require editing of different breeds and lines to maintain genetic diversity, and enable structured crossbreeding. This requirement is at odds with the process-based trigger and event-based regulatory approach that has been proposed for the products of genome editing by several countries. In the absence of regulatory harmony, researchers in some countries will have the ability to use genome editing in food animals, while others will not, resulting in disparate access to these tools, and ultimately the potential for global trade disruptions.

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“…In vitro gametogenesis from genetically modified stem cells could also be used as an efficient approach for genetic engineering (Soto & Ross, ). In addition, the in vitro production of gametes could reduce generation intervals and lead to new schemes for genetic improvement, such as in vitro breeding (IVB) (Goszczynski et al, ). The essential idea of IVB is to recapitulate in vitro the cycle of meiotic recombination and fertilization, and to combine it with genomic selection in each cycle in order to provide directional genetic progress.…”

Section: Introductionmentioning

confidence: 99%

“…This approach could be accomplished by isolation of embryonic stem cells from blastocysts, followed by genomic selection of the superior stem cells, in vitro generation of gametes, and subsequent IVF, resulting in a new generation of blastocyst‐stage embryos with potential for a subsequent round of selection. Based on simulations performed by Goszczynski et al (), this scheme could lead to genetic improvement 10 times faster than current breeding strategies, which are based on the intensive use of genomic selection and assisted reproductive technologies.…”

Section: Introductionmentioning

confidence: 99%

Gametes from stem cells: Status and applications in animal reproduction

Goszczynski

Denicol

Ross

2019

Reprod Domestic Animals

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In vitro gamete differentiation could revolutionize animal production by decreasing generation intervals, increasing the number of gametes per animal and facilitating the dissemination of elite genetics. In addition, it could help to develop new strategies for the conservation of endangered species. The recent in vitro reconstitution of germ cell development in mice has inspired researchers to invest their best efforts into reproducing this achievement in livestock species. With this goal in mind, multiple differentiation approaches and cell sources have been evaluated. The degree of success in these evaluations varies according to the species and the stage of development studied, but, in general, partially positive results have been obtained. Evidence suggests that although functional gametes with true reproductive potential are still to be obtained, it is a matter of time before this goal is achieved. K E Y W O R D S embryo, gametogenesis, stem cells | 23 GOSZCZYNSKI et al.

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In vitro breeding: application of embryonic stem cells to animal production†

Abstract: In vitro breeding constitutes a fast and intense selection strategy to genetically improve livestock populations.

Cited by 45 publications

References 114 publications

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Genome editing approaches to augment livestock breeding programs

Gametes from stem cells: Status and applications in animal reproduction

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