In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds

Abstract

The organogenic competence of leaf explants of eleven Carthamus species including C. tinctorius on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) + -naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) + NAA was investigated. Highly prolific adventitious shoot regeneration was observed in C. tinctorius and C. arborescens on both growth regulator combinations and the shoot regeneration frequency was higher on medium supplemented with TDZ + NAA. Nodal culture of nine Carthamus species on media supplemented with BA and kinetin (KIN) individually revealed the superiority of media supplemented with BA over that of KIN in facilitating a higher shoot proliferation index. Proliferating shoots from axillary buds and leaf explants were transferred to medium supplemented with 1.0 mg dm -3 KIN or 0.5 mg dm -3 BA for shoot elongation. Elongated shoots were rooted on half-strength MS medium supplemented with 1.0 mg dm -3 each of indole-butyric acid (IBA) and phloroglucinol. The plantlets thus obtained were hardened and transferred to soil.

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

“…This result can be explained with the presence of predetermined cells which first dedifferentiate themselves, subsequently form new meristematic centres, and finally differentiate again producing new organs [34][35][36][37][38][39]. This process, due to cell totipotency, has been observed in several species [30,[40][41][42][43][44][45][46][47] and is very efficient for regeneration. Our results showed that AFLP is a very sensitive and reliable molecular marker technique for revealing specific genomic alterations induced by tissue culture and for identifying slightly different genotypes.…”

Direct shoot regeneration from leaf explants of Spilanthes acmella

“…Direct regeneration from leaf, as another alternative step for clonal propagation and germplasm conservation, is a well established factor. Successful plant regeneration has been reported from leaf explants, e.g., in Indian spinach (Mitra and Mukherjee 2001), Plumbago species (Das and Rout 2002), safflower (Radhika et al 2006) and Carthamus species (Sujatha and Dinesh Kumar 2007). In view of the medicinal properties of S. acmella, its threatened nature and the increased demand for it in the pharmaceutical industry, there is a need for a large scale multiplication.…”

A Protocol for Rapid and High-Frequency In Vitro Propagation of Solanum nigrum L.

“…In addition, shoot regeneration from nodal segments by direct organogenesis in vitro has not yet been reported for S. nigrum. The cytokinins Kin and ZT (alone or in combination with auxins) are commonly used to induced callus formation and organogenesis in plant tissues (Kou et al 2012;Sujatha & Kumar 2007). In this study, all the cytokinins tested (KIN or ZT) could induced callus from petioles and rooted hypocotyls (Table 1).…”