In Vitro Bulbification of Five Lily Varieties: An Effective Method to Produce Quality Seeds and Flowers

Abstract

Lilies are one of the most important, beautiful, and economically valuable flowers in the world. Lilium is regarded as a popular floral trade cut flower, so viable protocols are needed to provide seed production, multiplication, and preservation. In vitro protocols allow for rapid large-scale production and rejuvenation of planting material, but to be a commercially viable multiplication method, the procedure must allow for rapid production of viable, true-to-type plants quickly. The objective was to evaluate the in vitro production of microbulbs of five lily varieties (Lilium “Champion Diamond,” Lilium “Yellow Diamond,” Lilium “Batavus,” Lilium “Hyde Park,” and Lilium sp.) using different concentrations of 6-benzylaminopurine (0, 0.5, 1.0, 1.5, 1.5, and 2.0 mg L−1 BAP) and to determine the commercial quality (flowering) of the plants grown from the seed obtained. Results from the micropropagation phase show Lilium “Batavus” and Lilium “Hyde Park” varieties had better in vitro responses, especially when grown with 1.0 and 1.5 mg L−1 BAP, respectively. Plants (of all varieties) grown from microbulbs showed positive growth and generally resulted in commercially viable flower production. Finally, the results of this study support the use of bulb scales as an alternative for obtaining vegetative seeds with high potential for lily cultivation.

Effects of Growth Regulator on Invitro Propagation of Lilium Using Bulb Scale

“…According to (Pandey, Singh, & Mamta Sharma, 2009), maximum proliferation has been recorded by micropropagation of Lilium using bulb scales as explants cultivated on MS medium supplemented with 0.50 mg/1 NAA and 2.0 mg/1 BAP. The Lilium "Batavus" and Lilium "Hyde Park" varieties exhibited better in vitro responses, especially when grown with 1.0 and 1.5 mg L1 BAP (Lapiz-Culqui et al, 2022). In the present study, different concentrations of IAA, NAA, or IBA-supplemented MS medium were utilized to promote root development.…”

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

“…They also found that the explant survival rate of basal scales was higher than that of tip bulb scales. Lapiz-Culqui et al [136] and Patil et al [137] used the bulb scales to cultivate different Lilium cultivars. Youssef et al [138] used the leaf as an explant in their study to increase Lilium bulb number and size.…”

A Review on Geophytes Micropropagation: Challenges and Opportunities

“…They also found that the explant survival rate of basal scales was higher than the tip bulb scales. Lapiz-Culqui, et al [124] and Patil, et al [125] used the bulb scales to cultivate different Lilium cultivars. Youssef, et al [126] used the leaf as explant in their study to increase Lilium bulb number and size.…”