In vitro cadmium removal from human serum by Cibacron Blue F3GA-thionein-complex conjugated affinity membranes

Denizli, Fatma; Deni̇zli̇, Adil; Ca, Meijers

doi:10.1002/(sici)1097-0126(200003)49:3<302::aid-pi366>3.0.co;2-r

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Cibacron Blue F3GA) were incorporated into these microspheres for separation and removal of several substances (e.g. proteins, pathogenic antibodies, cholesterol and toxic metal ions such as cadmium) from aqueous media including plasma [12][13][14][15][16][17][18]. The main criteria for PHEMA selection is its physiological acceptability, mechanical strength, chemical and biological stability and good blood-tollerability.…”

Section: Fibronectin Purification From Human Plasma In a Packedbed Comentioning

confidence: 99%

Fibronectin purification from human plasma in a packed-bed column system with gelatin immobilized PHEMA microspheres

Kayirhan-Denizli

Arıca

Deni̇zli̇

2001

Journal of Biomaterials Science, Polymer Edition

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Bioaffinity chromatography has a unique and powerful role that is used as a purification tool in the production of therapeutic plasma protein derivatives. In this study, a bioaffinity-ligand, i.e. gelatin, was covalently immobilized with PHEMA microspheres (150-200 microm in diameter). The affinity sorbent carrying 7.5 mg gelatin g(-1) polymer was then used to separate fibronectin from human plasma in a packed-bed column system. Fibronectin separation from human plasma on unmodified PHEMA microspheres was 0.45 mg g(-1), while much higher adsorption values, up to 21.8 mg g(-1), were obtained with gelatin-immobilized microspheres. The fibronectin adsorption capacity of the microspheres decreased with an increase in the recirculation rate of plasma. Fibronectin adsorption increased with decreasing temperature, and the maximum adsorption achieved at 4 degrees C (26.3 mg fibronectin g(-1)). Up to 94.7% of the adsorbed fibronectin was desorbed by using 2 M urea in the presence of 1 M sodium chloride as elution agent. The adsorption-desorption cycle was repeated ten times using the same affinity column. There was no remarkable reduction in the adsorption capacity of the gelatin-immobilized PHEMA microspheres.

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Section: Fibronectin Purification From Human Plasma In a Packedbed Comentioning

confidence: 99%

Fibronectin purification from human plasma in a packed-bed column system with gelatin immobilized PHEMA microspheres

Kayirhan-Denizli

Arıca

Deni̇zli̇

2001

Journal of Biomaterials Science, Polymer Edition

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Cadmium removal out of human plasma using ion-imprinted beads in a magnetic column

Candan

Tüzmen

Andaç

et al. 2009

Materials Science and Engineering: C

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Human serum albumin chromatography by Cibacron Blue F3GA-derived microporous polyamide hollow-fiber affinity membranes

Kassab

Yavuz

Odabaşı

et al. 2000

Journal of Chromatography B: Biomedical Sciences and Applicatio

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In vitro cadmium removal from human serum by Cibacron Blue F3GA-thionein-complex conjugated affinity membranes

Cited by 3 publications

References 20 publications

Fibronectin purification from human plasma in a packed-bed column system with gelatin immobilized PHEMA microspheres

Fibronectin purification from human plasma in a packed-bed column system with gelatin immobilized PHEMA microspheres

Cadmium removal out of human plasma using ion-imprinted beads in a magnetic column

Human serum albumin chromatography by Cibacron Blue F3GA-derived microporous polyamide hollow-fiber affinity membranes

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