In vitro callogenesis of Poincianella pyramidalis (catingueira)

Gomes-Copeland, Kicia Karinne Pereira; Lédo, A. da S.; David, Juceni P.; Araujo, Aparecida Gomes de; Almeida, Francisco A. G.

doi:10.1016/j.bjp.2016.12.005

Cited by 4 publications

(7 citation statements)

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“…Porém, eventos indesejáveis são enfrentados durante o estabelecimento dos materiais in vitro, como a contaminação e a oxidação. Para muitas espécies florestais, existem protocolos de desinfestação já desenvolvidos e em uso (Gomes-Copeland et al, 2017;Teixeira et al, 2018;Yarra et al, 2016). No entanto, para espécies nativas e endêmicas do cerrado, as pesquisas são inexistentes o que dificulta a propagação clonal e conservação desses materiais.…”

Section: Discussionunclassified

“…Respostas similares para a variável comprimento da parte aérea foram observadas por Gomes-Copeland et al (2017) e Vicente et al (2009), trabalhando com Conobea scoparioides e Vernonia condensata, respectivamente, com a concentração de 1 mg L -¹. O presente estudo revelou que, para D. mollis, doses maiores da citocinina BAP inibiram o alongamento da parte aérea. Possivelmente, essa resposta fisiológica pode ter ocorrido devido ao BAP não ser o regulador de crescimento responsável pelo alongamento celular (Cid, 2014).…”

Section: Discussionunclassified

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Óleos essenciais e benzilaminopurina (BAP) para o cultivo in vitro de Dimorphandra mollis

et al. 2021

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A micropropagação é uma alternativa para a conservação genética de espécies endêmicas do Cerrado, como Dimorphandra mollis (fava-d’anta), possibilitando a maximização da propagação em elevada qualidade. Para solucionar os fatores limitantes ao cultivo in vitro de D. mollis, o presente trabalho teve como objetivo avaliar a eficiência de óleos essenciais como sanitizante e testar concentrações de regulador de crescimento benzilaminopurina (BAP) na fase de multiplicação in vitro. Sementes coletadas em população natural foram escarificadas e desinfestadas com hipoclorito de sódio e óleos essenciais de Lippia origanoides, Ocimum gratissimum, L. rotundifolia e Cymbopogon citratus, sendo posteriormente inoculadas em meio de cultura MS. A multiplicação in vitro foi avaliada em quatro concentrações de BAP (0,5; 1,0; 1,5 e 2,0 mg L-1). O óleo essencial de C. citratus se mostrou o mais adequado para a desinfestação de sementes da fava d’anta, na fase de estabelecimento in vitro. Fitoreguladores utilizados no presente estudo não promoveram resultados satisfatórios para definir um protocolo de multiplicação in vitro para a espécie.

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Section: Discussionunclassified

Óleos essenciais e benzilaminopurina (BAP) para o cultivo in vitro de Dimorphandra mollis

et al. 2021

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“…Among responses associated with the physiological action of 2,4-D, we can mention callogenesis. The presence of calli in damaged plant tissue is widely used in plant tissue culture studies, such as the induction of somatic embryogenesis (GOMES-COPELAND et al, 2017;CIPRIANO et al, 2018;COSTA et al, 2020). Auxinic action refers to the stimulation of cell division, as verified in the responses observed in seeds and radicles of cell proliferation forming the callus.…”

Section: P á G I N a | 835mentioning

confidence: 99%

Effects of high-dose 2,4-D on germination and initial development of Canavalia ensiformis (L.) DC.

Silva

Costa

Direito

et al. 2022

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This study aimed to evaluate the development of Canavalia ensiformis seedlings under high concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) in different substrates. In the first analysis, seeds from different origins were disinfected and introduced into a modified Leonard vessel (PET vessel) containing washed sand or washed sand plus vermiculite substrates and under high concentrations of 2,4-D (0 - control, 250 and 500 mg/L). For a second analysis, seed germination, callogenesis and contamination in different concentrations of 2,4-D (0 - control, 20, 40, 250 and 500 mg/L) were evaluated with sterile sand as substrate. Results showed that higher concentrations of 2,4-D could induce larger areas of callus formation. At a concentration of 40 mg/L of 2,4-D, germination in riparian forest substrates washed sand resulted in root growth and callus formation without contamination. Although C. ensiformis seeds showed tolerance to regions with high concentrations of 2,4-D, they do not demonstrate phytoremediation capacity for this herbicide since it caused damage to the plant’s initial development.

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“…is a mediumsized arboreal species belonging to the family Fabaceae (Leguminosae), endemic to Brazil's semiarid northeastern region, with wide distribution in the Caatinga biome (Carvalho, 2014;Chaves et al, 2016;Souza et al, 2018). It is popularly known as 'catingueira', 'pau-de-rato', 'catingueira-das-folhas-largas', 'mussitaiba' and 'paude-porco' (Leite;Machado, 2009;Gomes-Copeland et al, 2017). The species has diverse potential uses, such as for wood and charcoal (Maia, 2004;Dias Júnior et al, 2018), for medicinal purposes (Chaves et al, 2016;Souza et al, 2018), for replanting of degraded areas and in agroforestry systems (Maia, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Poincianella pyramidalis naturally propagates sexually (Gomes-Copeland et al, 2017). However, its reproduction is often impaired by inadequate extractive practices, since its parts, especially the leaves, flowers and bark, are often used in folk medicine, and its wood is also used for various purposes (Maia, 2012).…”

Section: Introductionmentioning

confidence: 99%

In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

et al. 2019

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Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.

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In vitro callogenesis of Poincianella pyramidalis (catingueira)

Cited by 4 publications

References 10 publications

Óleos essenciais e benzilaminopurina (BAP) para o cultivo in vitro de Dimorphandra mollis

Óleos essenciais e benzilaminopurina (BAP) para o cultivo in vitro de Dimorphandra mollis

Effects of high-dose 2,4-D on germination and initial development of Canavalia ensiformis (L.) DC.

In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

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