In Vitro Cancer Cell Imaging, Free Radical Scavenging, and Fe3+ Sensing Activity of Green Synthesized Carbon Dots from Leaves of Piper longum

Mohapatra, Debadatta; Pratap, Ravi; Pandey, Vivek; Shreya, Singh; Senapati, Prakash Chandra; Dubey, Pawan K.; Parmar, Avanish Singh; Sahu, Alakh N.

doi:10.1007/s10876-022-02303-9

Cited by 9 publications

(8 citation statements)

References 64 publications

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“…The nanostructure of PLECDs, which allows for better penetration into the cells than PLLEE, may be the reason for their slightly greater cytotoxic potential. Our team previously reported a similar outcome (Mohapatra et al 2022).…”

Section: In-vitro Cytotoxicity and Bioimagingsupporting

confidence: 61%

“…CDs are widely studied for bioimaging, cancer theranostic, drug delivery, diagnosing various pathological conditions, gene therapy, photodynamic therapy, sensing, optoelectronic devices, catalysis, and many biological activities Jaleel et al 2018;Sciortino et al 2018;Mohapatra et al 2022). They can be produced mainly by two different approaches; top-down and bottom-up approaches.…”

Section: Introductionmentioning

confidence: 99%

“…The top-down approaches have disadvantages in participating in harsh conditions, necessitate expensive apparatus, and include various steps. Contrary to the above technique, the bottom-up technique-based solvothermal synthesis provides the bene ts of low cost, simplicity, easy scale-up potential, eco-friendly, and use of varied available carbon precursors (Mohapatra et al 2022). This method is widely explored for the preparation of CDs for multiple applications.…”

Section: Introductionmentioning

confidence: 99%

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Indian long pepper leaves-derived dual fluorescent carbon nano dots for environmental and health aspects

Mohapatra

Pratap

Pandey

et al. 2022

Preprint

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In this article, we present the synthesis of Piper longum leaves-derived ethanolic carbon dots (PLECDs) using the most simplistic environmentally friendly solvothermal carbonization method. The PLECDs fluoresced pink color with maximum emission at 670 nm at 397 nm excitation. Additionally, the dried PLECDs dissolved in water showed green fluorescence with higher emission at 452 nm at 370 nm excitation. The UV spectra showed peaks in the UV region (271.25 nm & 320.79 nm) and a noticeable tail in the visible region, signifying the efficient synthesis of nano-sized carbon particles and the Mie scattering effect. Various functional groups (-OH, -N-H, -C-H, -C = C, -C-N, and -C-O) were identified using Fourier-transform infrared spectroscopy (FTIR). Its nanocrystalline property was revealed by the sharp peaks in the X-ray diffraction (XRD). High-Resolution Transmission Electron Microscopy (HRTEM) photomicrograph displayed a roughly-spherical structure with a mean size of 2.835 nm. The Energy Dispersive X-ray (EDAX) spectra showed the presence of C, O, N, S, and P. The High-performance thin layer chromatography (HPTLC) fingerprint of PLECDs showed an altered pattern than its precursor (Piper longum leaves ethanolic extract or PLLEE). The PLECDs sensed Cu2+ selectively with a limit of detection (LOD) of 0.11 µM. It showed excellent cytotoxicity towards MDA-MB-231 (human breast cancer), SiHa (human cervical carcinoma), and B16F10 (murine melanoma) cell lines with excellent in-vitro bioimaging outcomes. It also has free radical scavenging activity. The PLECDs also showed outstanding bacterial biocompatibility, pH-dependent fluorescence stability, photostability, physicochemical stability, and thermal stability.

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Section: In-vitro Cytotoxicity and Bioimagingsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Indian long pepper leaves-derived dual fluorescent carbon nano dots for environmental and health aspects

Mohapatra

Pratap

Pandey

et al. 2022

Preprint

Self Cite

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“…The absorption peak at 734 nm was used to quantify the reduction of ABTS * + by H-donating antioxidants. [38] In addition, ABTS * + is dissolve in both water and a broad range of organic solvents, making it ideal for antioxidant assays of hydrophilic and hydrophobic materials. Chl-CQDs rapidly respond with ABTS * + and reach equilibrium quickly, but DPPH responds slowly and takes a longer time.…”

Section: Abts * + Assaymentioning

confidence: 99%

Therapeutic Potential of Chlorophyll Functionalized Carbon Quantum Dots against Cervical Cancer

et al. 2022

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Cervical cancer is an emerging gynecological disorder that affects the morbidity and mortality of the females worldwide. In 2018, according to the World Health Organization, 570,000 women were diagnosed with cervical cancer and 311,000 women died from the condition. Various anticancer agents are in the run to combat cervical cancer but overall results are still demoralizing. Various reports suggest that Quantum dots are being used as promising agents against several types of cancer.In the current study, we synthesized high-quantum yield chlorophyll functionalized carbon quantum dots (Chl-CQDs) from banana leaves with a simple and environment friendly hydrothermal process. The free radical activity of Chl-CQDs was measured using diphenyl picrylhydrazyl (DPPH) and azino-bis (ethylbenzothiazoline sulphonic acid) diammonium salt (ABTS * + ), as well as their cytotoxic, bioimaging, and apoptogenic properties were also evaluated on normal cell line HEK-293 and human cancer cell lines SiHa. Our results of dimethylthiazol diphenyltetrazolium bromide tetrazolium reduction (MTT) assay showed that Chl-CQDs have profound effect on SiHa cells with an IC 50 of approximately 100 μg/ml after 24 hours and no effect HEK-293 cells. Bioimaging and apoptogenic assays confirmed that Chl-CQDs exhibited strong therapeutic potential against SiHa cells and no cytotoxic potential against HEK-293 cells. The cumulative findings suggested that Chl-CQDs exhibited non-cytotoxic nature against HEK-293 cells whereas cytotoxic nature against SiHa cells.

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“…In 2015, Zhao et al developed a bifunctional fluorescent CD with garlic as the natural precursor, which showed the DPPH radical (DPPH • ) scavenging activity for the first time. Subsequently, various biomass CDs with radical scavenging activity have also been successfully derived from natural plants such as pomelo, black soya beans, Piper longum, Carica papaya, and onion . However, due to the unsatisfactory antioxidant properties, few biomass CDs have been adopted for free-radical scavenging in vivo .…”

Section: Introductionmentioning

confidence: 99%

Hydrothermally Derived Green Carbon Dots from Broccoli Water Extracts: Decreased Toxicity, Enhanced Free-Radical Scavenging, and Anti-Inflammatory Performance

Deng

Zang

et al. 2023

ACS Biomater. Sci. Eng.

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Biomass carbon dots (CDs) derived from natural plants possess the advantages of low cost, photostability, and excellent biocompatibility, with potential applications in chemical sensing, bioimaging, and nanomedicine. However, the development of biomass CDs with excellent antioxidant activity and good biocompatibility is still a challenge. Herein, we propose a hypothesis for enhancing the antioxidant capacity of biomass CDs based on precursor optimization, extraction solvent, and other conditions with broccoli as the biomass. Compared to broccoli water extracts, broccoli powders, and broccoli organic solvent extracts, CDs derived from broccoli water extracts (BWE-CDs) have outstanding antioxidant properties due to the abundant CC, carbonyl, and amino groups on their surface. After optimization of the preparation condition, the obtained BWE-CDs exhibit excellent free-radical scavenging activity with an EC50 of 68.2 μg/mL for DPPH• and 22.4 μg/mL for ABTS•+. Cytotoxicity and zebrafish embryotoxicity results indicated that BWE-CDs have lower cytotoxicity and better biocompatibility than that of CDs derived from organic solvents. In addition, BWE-CDs effectively scavenged reactive oxygen species (ROS) in A549 cells, 293T cells, and zebrafish, as well as eliminating inflammation in LPS-stimulated zebrafish. Mechanistic studies showed that the anti-inflammatory effect of BWE-CDs was dependent on the direct reaction of CDs with free radicals, the regulation of NO levels, and the upregulation of the expression of SOD and GPX-4. This work indicates that the antioxidant activity of CDs could be enhanced by using solvent extracts of biomass as precursors, and the obtained BWE-CDs exhibit characteristics of greenness, low toxicity, and excellent antioxidant and anti-inflammatory activities, which suggests the potential promising application of BWE-CDs as an antioxidant nanomedicine for inflammatory therapy.

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In Vitro Cancer Cell Imaging, Free Radical Scavenging, and Fe3+ Sensing Activity of Green Synthesized Carbon Dots from Leaves of Piper longum

Cited by 9 publications

References 64 publications

Indian long pepper leaves-derived dual fluorescent carbon nano dots for environmental and health aspects

Indian long pepper leaves-derived dual fluorescent carbon nano dots for environmental and health aspects

Therapeutic Potential of Chlorophyll Functionalized Carbon Quantum Dots against Cervical Cancer

Hydrothermally Derived Green Carbon Dots from Broccoli Water Extracts: Decreased Toxicity, Enhanced Free-Radical Scavenging, and Anti-Inflammatory Performance

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