In vitro cellular and humoral responses to Schistosoma mansoni vaccine candidate antigens

Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells

“…Therefore, it is important to select the least conserved peptides of schistosome antigens, such as Sm14 and paramyosin, to be used in a vaccine formulation. In a recent study performed in an endemic area for schistosomiasis in Egypt, cellular immune response to Sm14 and paramyosin was related with resistance to reinfection (Al-Sherbiny et al 2003). These findings reinforce the importance of identifying T cell epitopes on these antigens that may be involved in the induction of protective immunity.…”

Identification of paramyosin T cell epitopes associated with human resistance toSchistosoma mansonireinfection

“…This assay showed that Para (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29), Para (210-226) and Para (355-371) were able to bind to 100% (8/8) of the HLA-DR molecules used in this assay,…”

“…PBMC of 14% of individuals in this study did not proliferate in response to paramyosin peptides (data not shown). Para (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) was the most promiscuous peptide according to the proliferative responses. PBMC from 20% of the individuals tested proliferated in response to this epitope ( Table 2).…”

“…However, we believe that preferential recognition of Para (210-226) by the RR group compared to the SR group cannot be attributed to immunomodulation mediated by IL-10 produced by the SR group, as no statistically significant difference was observed between both groups regarding proliferative responses to PHA. Because the human immune response to paramyosin has been associated with resistance to S. mansoni infection and reinfection [11][12][13], this peptide might represent one possible component responsible to induce resistance in individuals living in areas endemic for schistosomiasis. Additionally, Para (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) and Para (355-371) peptides induced T cell responses in 20% and 14·6% of tested individuals, respectively, and bound to six and eight of the HLA alleles studied in the in vitro HLA-binding assay.…”

“…These results indicate the immunological relevance of these epitopes as potential vaccine candidates. Furthermore, Para (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) possesses only three amino acids that are different from the Taenia solium paramyosin B cell epitope identified in a phage display library [27], and may suggest Para (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) as a potential B cell epitope.…”