In vitro characterisation of 3D printed platelet lysate-based bioink for potential application in skin tissue engineering

Daikuara, Luciana Y.; Yue, Zhilian; Skropeta, Danielle; Wallace, Gordon G.

doi:10.1016/j.actbio.2021.01.021

Cited by 42 publications

(36 citation statements)

References 60 publications

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“…The bioprinted samples were incubated in PBS at 37 • C for 24 h. Then, the samples were removed from the PBS, and the wet weight [75] of the samples was determined. Then, samples were lyophilized for 24 h and the dry weight (W d ) was determined [51,55]. The swelling ratios of the hydrogels were calculated by Equation (2).…”

Section: Swelling Ratio Of Bioprinted Constructs With and Without Microspheresmentioning

confidence: 99%

“…The swelling capacity of the printed construct was not affected by pH or the addition of platelet lysate, since platelet lysate and gelatin methacryloyl (GelMA) presented almost analogous swelling behaviors. GelMA is a semi-synthetic hydrogel that is similar to the extracellular matrix of native tissues and made up of gelatin derivatized with methacrylamide and methacrylate groups [55].…”

Section: Introductionmentioning

confidence: 99%

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Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

et al. 2021

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Three-dimensional bioprinting can fabricate precisely controlled 3D tissue constructs. This process uses bioinks—specially tailored materials that support the survival of incorporated cells—to produce tissue constructs. The properties of bioinks, such as stiffness and porosity, should mimic those found in desired tissues to support specialized cell types. Previous studies by our group validated soft substrates for neuronal cultures using neural cells derived from human-induced pluripotent stem cells (hiPSCs). It is important to confirm that these bioprinted tissues possess mechanical properties similar to native neural tissues. Here, we assessed the physical and mechanical properties of bioprinted constructs generated from our novel microsphere containing bioink. We measured the elastic moduli of bioprinted constructs with and without microspheres using a modified Hertz model. The storage and loss modulus, viscosity, and shear rates were also measured. Physical properties such as microstructure, porosity, swelling, and biodegradability were also analyzed. Our results showed that the elastic modulus of constructs with microspheres was 1032 ± 59.7 Pascal (Pa), and without microspheres was 728 ± 47.6 Pa. Mechanical strength and printability were significantly enhanced with the addition of microspheres. Thus, incorporating microspheres provides mechanical reinforcement, which indicates their suitability for future applications in neural tissue engineering.

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Section: Swelling Ratio Of Bioprinted Constructs With and Without Microspheresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

et al. 2021

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“…Plant‐derived biomaterials, such as cellulose, [ 95 ] and pectin [ 96 ] also have been investigated to develop bioinks for skin bioprinting. Marine‐sourced biomaterials including alginate, [ 93a,94,97 ] chitosan, [ 98 ] and ulvan, [ 99 ] and human growth factor‐rich biomaterials including platelet lysate [ 100 ] are also gaining increasing attention in tissue engineering. Synthetic polymers commonly used in bioink formulations include polyethylene glycol (PEG), [ 91b,101 ] pluronic, [ 102 ] poly(vinyl pyrrolidone) (PVP), [ 92b ] and polycaprolactone (PCL).…”

Section: Skin Wound Healingmentioning

confidence: 99%

“…[ 120 ] The use of GelMA as bioink components for skin bioprinting has been widely reported. [ 91a,99b,100,110 ] Examples include Shi et al. who have described a bioink composite made of GelMA and collagen doped with tyrosinase for 3D bioprinting of living skin tissues.…”

Section: Skin Wound Healingmentioning

confidence: 99%

“…As platelet lysate lacks mechanical robustness and is unable to form extruded filaments, GelMA was incorporated into the bioink formulation to meet rheological characteristics appropriate to the printing method. [ 100 ] Nonetheless, the use of platelet bioproducts as a component of a bioink formulation for skin printing has yet to be fully explored.…”

Section: Skin Wound Healingmentioning

confidence: 99%

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3D Bioprinting Constructs to Facilitate Skin Regeneration

Daikuara

Chen

Yue

et al. 2021

Adv Funct Materials

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In the last decade, the advent of 3D printing for tissue engineering and regenerative medicine has engendered great interest for those involved in skin repair and regeneration. 3D bioprinting allows spatial distribution of skin cells into predefined custom‐made structures to produce living skin mimics on the bench for grafting or drug testing. The key aspect of 3D bioprinting lies in the formulation of printable bioinks serving as matrix mimics to house skin cells, alongside an appropriate combination of cells. In this review, bioink formulations, cell combinations, as well as manufacturing methods exploited to develop 3D bioprinted constructs for skin regeneration are summarized. Issues to do with the selection of suitable materials and cells to ensure the functionality of the resulting skin constructs and fabrication of skin appendages are also addressed.

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Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches

Sobreiro‐Almeida,

Santos,

Decarli

et al. 2024

Advanced Science

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This study proposes a tunable ink engineering methodology to allow 3D printing processability of highly bioactive but otherwise low‐viscous and unprintable blood‐derived materials. The hypothesis relies on improving the viscoelasticity and shear thinning behavior of platelet lysates (PL) and albumins (BSA) solutions by covalent coupling, enabling simultaneous extrusion and photocrosslinking upon filament deposition. The available amine groups on proteins (PL and BSA) are exploited for coupling with carboxyl groups present in methacrylated proteins (hPLMA and BSAMA), by leveraging carbodiimide chemistry. This reaction enabled the creation of a pre‐gel from these extremely low‐viscous materials (≈ 1 Pa), with precise tuning of the reaction, resulting in inks with a range of controlled viscosities and elasticities. Shape‐fidelity analysis is performed on 3D‐printed multilayered constructs, demonstrating the ability to reach clinically relevant sizes (>2 cm in size). After photocrosslinking, the scaffolds showcased a mechanically robust structure with sustained protein release over time. Bioactivity is evaluated using human adipose‐derived stem cells, resulting in increased viability and metabolic activity over time. The herein described research methodology widens the possibilities for the use of low‐viscosity materials in 3D printing but also enables the direct application of patient and blood‐derived materials in precision medicine.

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In vitro characterisation of 3D printed platelet lysate-based bioink for potential application in skin tissue engineering

Cited by 42 publications

References 60 publications

Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

3D Bioprinting Constructs to Facilitate Skin Regeneration

Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches

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