In-vitro characterisation of the four lineages of Phytophthora ramorum

O’Hanlon, Richard; Choiseul, J.W.; Grogan, Helen; Brennan, James M.

doi:10.1007/s10658-016-1019-2

Cited by 12 publications

(14 citation statements)

References 32 publications

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“…This difference in experiment 1 was consistent with the measures of sporulation, with more sporangia being produced by EU1 lesions in experiment 1. The combination of larger lesions and a greater number of sporangia per unit of lesion length observed for EU1 in experiment 1 is consistent with reports for rhododendron (Denman et al, 2005;Elliot et al, 2011;Manter et al, 2010;O'Hanlon et al, 2017). This suggests a potentially greater impact on susceptible forest species within the area of the EU1 outbreak.…”

Section: Ta B L Esupporting

confidence: 89%

Characterizing the variation in aggressiveness and sporulation of the NA1 and EU1 lineages of Phytophthora ramorum in Oregon

Søndreli

Kanaskie²,

Navarro

et al. 2021

Plant Pathology

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Section: Ta B L Esupporting

confidence: 89%

Characterizing the variation in aggressiveness and sporulation of the NA1 and EU1 lineages of Phytophthora ramorum in Oregon

Søndreli

Kanaskie²,

Navarro

et al. 2021

Plant Pathology

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“…The details of the isolates used in this study are provided in Table 1. Methods for the isolation of the isolates and tentative morphological identification have been reported previously [41,42]. Briefly, this involved plating the samples onto P 5 ARP[H] agar (Cornmeal agar with antibiotics [43]) and incubating at room temperature for two weeks.…”

Section: Isolation and Culturingmentioning

confidence: 99%

Comparative Genomic and Proteomic Analyses of Three Widespread Phytophthora Species: Phytophthora chlamydospora, Phytophthora gonapodyides and Phytophthora pseudosyringae

et al. 2020

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The Phytophthora genus includes some of the most devastating plant pathogens. Here we report draft genome sequences for three ubiquitous Phytophthora species—Phytophthora chlamydospora, Phytophthora gonapodyides, and Phytophthora pseudosyringae. Phytophthora pseudosyringae is an important forest pathogen that is abundant in Europe and North America. Phytophthora chlamydospora and Ph. gonapodyides are globally widespread species often associated with aquatic habitats. They are both regarded as opportunistic plant pathogens. The three sequenced genomes range in size from 45 Mb to 61 Mb. Similar to other oomycete species, tandem gene duplication appears to have played an important role in the expansion of effector arsenals. Comparative analysis of carbohydrate-active enzymes (CAZymes) across 44 oomycete genomes indicates that oomycete lifestyles may be linked to CAZyme repertoires. The mitochondrial genome sequence of each species was also determined, and their gene content and genome structure were compared. Using mass spectrometry, we characterised the extracellular proteome of each species and identified large numbers of proteins putatively involved in pathogenicity and osmotrophy. The mycelial proteome of each species was also characterised using mass spectrometry. In total, the expression of approximately 3000 genes per species was validated at the protein level. These genome resources will be valuable for future studies to understand the behaviour of these three widespread Phytophthora species.

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“…Furthermore, our inability to rank hosts properly for susceptibility and infectivity has made regulations less effective. A further complication has been added by the presence of at least four genetically distinct lineages of the pathogen affecting ornamental stock (Ivors et al, 2006;Van Poucke et al, 2012), which reportedly differ in their aggressiveness on plant hosts (Elliott et al, 2011;Eyre et al, 2014;Manter et al, 2010;O'Hanlon et al, 2017). The relative abundance of lineages in the ornamental plant industry varies with time and location (Grünwald et al, 2019), making long-term studies on the impact each lineage may have, particularly complex.…”

Section: P Ramorum Has Been Unwittingly Introduced Into Both Europementioning

confidence: 99%

“…Predictions based on data from studying the spread and impact of a single lineage may not apply when more than one lineage is present, and while interactions between P. ramorum and other co-mingling Phytophthora species have been studied (Kozanitas et al, 2017), the outcome of interactions among genotypes belonging to different lineages is still widely unknown, with the exception of a few studies on their sexual reproductive potential (Boutet et al, 2010;Vercauteren et al, 2011). A few studies have compared lineages using inoculation trials (Elliott et al, 2011;Eyre et al, 2014;Manter et al, 2010;O'Hanlon et al, 2017); however, these were carried out on a rather small number of hosts, thus a true understanding of the differences among these lineages is still lacking.…”

Section: P Ramorum Has Been Unwittingly Introduced Into Both Europementioning

confidence: 99%

Pathogenicity and infectivity of Phytophthora ramorum vary depending on host species, infected plant part, inoculum potential, pathogen genotype, and temperature

2020

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A total of 25 ornamental plant species representing 10 families were inoculated using three genotypes, each representing one of the genetic lineages NA1, NA2, and EU1 of the pathogen Phytophthora ramorum. Leaves were inoculated using suspensions with two zoospore concentrations and exposure at three temperatures, while stems were inoculated using agar plugs colonized by mycelia. Susceptibility was determined by measuring either the success of pathogen reisolation or lesion length caused by the pathogen. Infectivity was determined by counting sporangia in washes of inoculated leaves or stems. Results from all three pathogen genotypes combined were used to rank each of the 25 plant species for susceptibility and infectivity, while pooled results per genotype from all 25 hosts combined were employed for a preliminary comparison of pathogenicity and infectivity among genotypes. Statistical analyses showed that leaf results were affected by the concentration of zoospores, temperature, plant host, pathogen genotype, and by the interaction between host and pathogen genotype. Stem results were mostly affected by host and by the interaction between host and pathogen genotype. Hosts ranked differently when looking at the various parameters, and differences in rankings were also significant when comparing stem and leaf results. Differences were identified among the 25 hosts and the three pathogen genotypes for all parameters: results can be used for decision‐making regarding regulations or selection of plants to be grown where infestations by P. ramorum are an issue.

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In-vitro characterisation of the four lineages of Phytophthora ramorum

Cited by 12 publications

References 32 publications

Characterizing the variation in aggressiveness and sporulation of the NA1 and EU1 lineages of Phytophthora ramorum in Oregon

Characterizing the variation in aggressiveness and sporulation of the NA1 and EU1 lineages of Phytophthora ramorum in Oregon

Comparative Genomic and Proteomic Analyses of Three Widespread Phytophthora Species: Phytophthora chlamydospora, Phytophthora gonapodyides and Phytophthora pseudosyringae

Pathogenicity and infectivity of Phytophthora ramorum vary depending on host species, infected plant part, inoculum potential, pathogen genotype, and temperature

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