In vitro chemotaxis and tissue remodeling assays quantitatively characterize foreign body reaction

Jannasch, Maren

doi:10.14573/altex.1610071

Cited by 15 publications

(18 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, even if a transition occurs, for example from M2 to M1, the cell characteristics of putatively switched M2 macrophages strongly differ from initially M1-polarized cultures. Although, a switch between macrophage phenotypes is theoretically possible, in our previous studies we could not detect a switch after only 48 h of culture, especially without external stimulation of the macrophages [3,4].…”

Section: Discussionmentioning

confidence: 59%

“…Mononuclear cells from peripheral blood (University Hospital Wuerzburg, Germany) were isolated according to a previously published protocol [4]. Briefly, following ficoll gradient centrifugation (GE Healthcare, Freiburg, Germany), monocytes were separated by a negative magnetic cell separation (MiltenyiBiotec, Bergisch Gladbach, Germany) to remove T cells, NK cells, B cells, dendritic cells, and basophils.…”

Section: Isolation and Differentiation Of Human Monocytes To Macrophagesmentioning

confidence: 99%

“…Thus, the FBR is a key factor in the long-term survival and function of an implanted biomaterial [2]. During the FBR, macrophages play a major role [3,4]. Over time, an initial population of short-lived pro-inflammatory M1 macrophages is replaced by long-vitae M2 macrophages.…”

Section: Introductionmentioning

confidence: 99%

“…Over time, an initial population of short-lived pro-inflammatory M1 macrophages is replaced by long-vitae M2 macrophages. The chronic accumulation and fusion of these M2 macrophages in the proximity of the implant induces the production of a dense fibrous capsule by fibroblasts, isolating the foreign body from the native tissue [4].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages

et al. 2020

Self Cite

View full text Add to dashboard Cite

Implants elicit an immunological response after implantation that results in the worst case in a complete implant rejection. This biomaterial-induced inflammation is modulated by macrophages and can be influenced by nanotopographical surface structures such as titania nanotubes or fractal titanium nitride (TiN) surfaces. However, their specific impact on a distinct macrophage phenotype has not been identified. By using two different levels of nanostructures and smooth samples as controls, the influence of tubular TiO2 and fractal TiN nanostructures on primary human macrophages with M1 or M2-phenotype was investigated. Therefore, nanotopographical coatings were either, directly generated by physical vapor deposition (PVD) or by electrochemical anodization of titanium PVD coatings. The cellular response of macrophages was quantitatively assessed to demonstrate a difference in biocompatibility of nanotubes in respect to human M1 and M2-macrophages. Depending on the tube diameter of the nanotubular surfaces, low cell numbers and impaired cellular activity, was detected for M2-macrophages, whereas the impact of nanotubes on M1-polarized macrophages was negligible. Importantly, we could confirm this phenotypic response on the fractal TiN surfaces. The results indicate that the investigated topographies specifically impact the macrophage M2-subtype that modulates the formation of the fibrotic capsule and the long-term response to an implant.

show abstract

Section: Discussionmentioning

confidence: 59%

Section: Isolation and Differentiation Of Human Monocytes To Macrophagesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…This view was further supported by the macroscopic observation that the CCR2-knockout animals also did not exhibit prolonged wound healing following the surgical incisions performed for the implantations. However, interactions between macrophages and the fibroblast response have been documented before (44,45), emphasizing the necessity of myeloid cells to orchestrate the wound healing response (46,47). As we demonstrate by the in vitro fibroblast activation assay, tissues derived from CCR2-knockout animals exhibited a lower potential for fibroblast activation compared with the corresponding WT controls (8, 10) but still induced enhanced fibroblast activation compared with the untreated control cells.…”

Section: Discussionmentioning

confidence: 67%

Polypropylene mesh implantation for hernia repair causes myeloid cell–driven persistent inflammation

Heymann¹,

Trotha²,

Preisinger³

et al. 2019

JCI Insight

View full text Add to dashboard Cite

Biomimetic Connection of Transcutaneous Implants with Skin

Weigel,

Christ,

Dembski

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

Bacterial infection is a crucial complication in implant restoration, in particular in permanent skin‐penetrating implants. Therein, the resulting gap between transcutaneous implant and skin represents a permanent infection risk, limiting the field of application and the duration of application. To overcome this limitation, a tight physiological connection is required to achieve a biological and mechanical welding for a long‐term stable closure including self‐healing probabilities. This study describes a new approach, wherein the implant is connected covalently to a highly porous electrospun fleece featuring physiological dermal integration potential. The integrative potential of the scaffold is shown in vitro and confirmed in vivo, further demonstrating tissue integration by neovascularization, extracellular matrix formation, and prevention of encapsulation. To achieve a covalent connection between fleece and implant surface, self‐initiated photografting and photopolymerization of hydroxyethylmethacrylate is combined with a new crosslinker (methacrylic acid coordinated titanium‐oxo clusters) on proton‐abstractable implant surfaces. For implant modification, the attached fleece is directed perpendicular from the implant surface into the surrounding dermal tissue. First in vitro skin implantations demonstrate the implants' dermal integration capability as well as wound closure potential on top of the fleece by epithelialization, establishing a bacteria‐proof and self‐healing connection of skin and transcutaneous implant.

show abstract

In vitro chemotaxis and tissue remodeling assays quantitatively characterize foreign body reaction

Cited by 15 publications

References 34 publications

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages

Polypropylene mesh implantation for hernia repair causes myeloid cell–driven persistent inflammation

Biomimetic Connection of Transcutaneous Implants with Skin

Contact Info

Product

Resources

About