In vitro cleavage of bovine and ovine early embryos: Improved development using coculture with trophoblastic vesicles

Heyman, Y.; Ménézo, Yves; Chesné, P.; Camous, Sylvaine; Garnier, V.

doi:10.1016/0093-691x(87)90070-7

Cited by 133 publications

(45 citation statements)

References 17 publications

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“…Therefore, we believe that t h e b l a s t o c y t d e v e l o p m e n t r a t e w a s n o t s u b s t a n t i a l l y i n f l u e n c e d b y t h e m e d i u m replacements on days 5-6 post IVF because the 8-cell block barrier had previously invalidated this procedure. Several methods have been reported for overcoming the 8-cell block , such as co-culture systems with granulosa [19][20][21], oviduct epithelial cells [11,12], trophoblastic tissues [22], addition of growth factors [23][24][25][26][27], glucose deprivation [28], culture under low oxygen concentration [29,30], addition of reducing agents [13], and cytokines [32,33]. Particularly in culture systems that replace coculture with somatic cells, methods employing a conditioned medium of bovine oviduct epithelial cells [11,12], buffalo rat hepatocytes [13], bovine granulosa cells, and vero cells [14] have been documented.…”

Section: Discussionmentioning

confidence: 99%

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Fujita

Umeki

Shimura

et al. 2006

Journal of Reproduction and Development

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Abstract.We investigated the effect of group culture on bovine embryo development, and also investigated the effect of embryo-culture conditioned medium on developmental competence of individually cultured bovine embryos. Slaughterhouse-derived bovine oocytes were matured and fertilized in vitro. The presumptive zygotes were cultured individually or cultured in groups of 2 to 5 embryos with a constant culture density (5 µl/embryo). After 7 days of culture, the rates of embryos developed to the blastocyst stage were significantly higher (P<0.05) in group cultures of more than 3 embryos/drop than for embryo culture of 1 or 2 embryos/drop. These results suggest a beneficial effect of group culture may be exerted by possible growth promoting factors secreted by embryos. In the next experiment, we investigated the effect of timing of fresh medium replacement on the development of embryos cultured in groups. The blastocyst formation rate was lower when culture medium was replaced freshly on days 2-4 after fertilization than on days 5-6. The blastocyst formation rates of single-cultured embryos were significantly (p<0.05) increased by the addition of conditioned medium derived from multiple-embryo culture. These results indicate that group culture promotes embryo development and that embryo culture-derived conditioned medium is effective for supporting development of single cultured embryos. Key words: Bovine, Conditioned medium, Development, Embryo, Single embryo (J. Reprod. Dev. 52: [137][138][139][140][141][142] 2006) ecently, ultrasound-guided ovum pick-up (OPU) has enabled repeat collection of oocytes from live cattle for genetic improvement [1][2][3]. However, the efficiency of embryo production by OPU is still low because of the limited number of oocytes collected and used for in vitro fertilization (IVF) and culture (IVC). In our field data, the blastocyst formation rate decreased when the number of in vitro fertilized oocytes collected from individual heifers was less than 3. Therefore, it is important to increase the efficiency of blastocyst production from small numbers of in vitro fertilized oocytes collected by OPU.Previous studies in mice [4, 5], sheep [6], and cattle [7][8][9] have demonstrated that group cultures promote embryo development compared with single-culture or co-culture with small numbers of embryos. In murine embryos, the blastocyst formation rate is promoted by an increase of embryo density in culture drops [10], suggesting that specific factor(s) are secreted from the embryos to influence embryo growth and development in an autocrine or paracrine manner. In the non-coculture system, embryo development is promoted by adding conditioned medium derived from bovine oviductal epithelial cells [11,12], buffalo/rat hepatocytes [13], bovine granulosa cells, or Vero

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Section: Discussionmentioning

confidence: 99%

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Fujita

Umeki

Shimura

et al. 2006

Journal of Reproduction and Development

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“…Oviducal epithelial cells have also been shown to maintain the motility and fertilizing capacity of bovine spermatozoa in vitro (Pollard et al, 1991). Various other cell types including trophoblastic vesicles (Camous et ai, 1984;Heyman et al, 1987), a trophoblastic cell strain (Scodras et al, 1990), cumulus cells (Goto et ai, 1988), embryonic chicken cells (Blakewood et al, 1988), uterine cells (Voelkel et al, 1985;Wiemer et al, 1989), fibroblast cells (Gandolfi & Moor, 1987), epididymal epithelial cells (J. W. Pollard, personal communication) and a transformed monkey-kidney cell line (VERO; Ménézo et al, 1990) have been used to facilitate the critical transition from maternal to embryonic control of early cleavage (Barnes & Eyestone, 1990). This implies that any factor(s) that may exist is probably not tissue specific.…”

Section: Methodsmentioning

confidence: 99%

Development and viability of bovine embryos derived from oocytes matured and fertilized in vitro and co-cultured with bovine oviducal epithelial cells

Xu¹,

Yadav²,

Rorie³

et al. 1992

Reproduction

166

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“…The development of early bovine embryos derived ex vivo was improved upon by co-culture with trophoblastic vesicles derived ex vivo [13,14]. The preparation of trophoblastic vesicles derived in vitro by a simple culture method would be more practically suitable for future studies.…”

Section: Introductionmentioning

confidence: 99%

“…The development of embryos is promoted by recombinant interferon-τ, with its receptor expressed in morula-stage embryos [12]. Embryos effectively develop upon co-culture with trophoblastic vesicles derived from ex vivo cultures [13,14]. Stojkovic et al [15,16] reported that either interferon-τ secreted from trophoblastic vesicles or trophoblastic vesicle-conditioned medium improves the development of bovine embryos in vitro.…”

Section: Introductionmentioning

confidence: 99%

Development of a Single Bovine Embryo Improved by Co-culture with Trophoblastic Vesicles in Vitamin-supplemented Medium

Mori

Kasa²,

Hattori

et al. 2012

Journal of Reproduction and Development

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Abstract.To improve the development of singly cultured bovine embryos, we developed a co-culture method with trophoblastic vesicles. The growth of trophoblastic cells was markedly increased in vitamin-supplemented medium 199 compared with medium 199. Upon co-culture of a single embryo with trophoblastic vesicles in vitamin-supplemented medium 199, embryo development to the blastocyst stage was significantly higher than in embryos co-cultured with trophoblastic vesicles in RPMI 1640 or with cumulus cells in medium 199 (control). In the absence of the vitamin cocktail, co-culture with trophoblastic vesicles in medium 199 did not improve embryo development compared with that of the control. The vitamin cocktail was effective in embryo development when co-cultured with trophoblastic vesicles, but not with cumulus cells. Embryo development was not improved in the absence of co-cultured trophoblastic vesicles, even in the presence of vitamin cocktail. In conclusion, the co-culture system with trophoblastic vesicles in vitamin-supplemented medium 199 efficiently enhances the development of singly cultured embryos. Key words: Bovine, Co-culture, Embryo, Trophoblast, Vitamins (J. Reprod. Dev. 58: [717][718][719][720][721] 2012) T here has long been the need for a specific culture system for use with a small number of oocytes collected individually from one or both ovaries of a bovine under the guidance of an ultrasonic apparatus. Generally, the development of singly or sparsely cultured early embryos is poor compared with embryos cultured in larger numbers [1,2]. An individually cultured oocyte becomes well developed upon co-culturing with granulosa cells, but blastocyst formation remains low [3]. Fujita et al. [4] suggested that embryo culture medium was effective for development of an individually cultured embryo. Larson et al. [5] reported that interferon-τ was more highly secreted from embryos cultured in groups compared with an individually cultured embryo. It is well known that interferon-τ secreted from trophoblastic cells is an important signal of maternal recognition during pregnancy [6][7][8][9][10]. Interferon-τ mRNA is detected at the blastocyst stage, and interferon-τ is first produced after blastocyst expansion followed by hatching from the zona pellucida [11]. The development of embryos is promoted by recombinant interferon-τ, with its receptor expressed in morula-stage embryos [12]. Embryos effectively develop upon co-culture with trophoblastic vesicles derived from ex vivo cultures [13,14]. Stojkovic et al. [15,16] reported that either interferon-τ secreted from trophoblastic vesicles or trophoblastic vesicle-conditioned medium improves the development of bovine embryos in vitro. Thus, trophoblasts appear to have a potential role in the development of early embryos through a mechanism of enhanced interferon-τ secretion.Trophoblastic vesicles can be prepared from embryo cultures in vitro, as well as from the ruminant conceptus after collection from the uterus [17], where interferon-τ is secreted from...

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In vitro cleavage of bovine and ovine early embryos: Improved development using coculture with trophoblastic vesicles

Cited by 133 publications

References 17 publications

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Development and viability of bovine embryos derived from oocytes matured and fertilized in vitro and co-cultured with bovine oviducal epithelial cells

Development of a Single Bovine Embryo Improved by Co-culture with Trophoblastic Vesicles in Vitamin-supplemented Medium

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