In vitro clinical‐grade generation of red blood cells from human umbilical cord blood CD34+ cells

Abstract

This study is the first report on generating clinical-grade RBCs by in vitro culture with human MSCs and compared effectiveness of several cytokines for RBC production. This provides a useful basis for future production of clinically available RBCs and a model of erythropoiesis that is analogous to the in vivo system.

The potential of human peripheral blood derived CD34+ cells for ex vivo red blood cell production

“…For full terminal maturation coculture on human (Baek et al, 2008) or murine stromal feeder cells (Giarratana et al, 2005;Vlaski et al, 2009) or co-culture with macrophages (Fujimi et al, 2008) have been employed but successful enucleation in the absence of stromal support has also been reported (Miharada et al, 2006). Best results have been achieved with cord blood derived CD34+ cells and most research has focused on this source of haematopoietic stem cells due to its higher expansion potential (Fujimi et al, 2008;Miharada et al, 2006).…”

Characterization of Different Subpopulations from Bone Marrow-Derived Mesenchymal Stromal Cells by Alkaline Phosphatase Expression

“…Cord blood MSCs were isolated and cultured as previously described [23]. As control cells for differentiation assay, primary osteoblasts and preadipocytes (3T3-L1) were used.…”

Proof of principle for transfusion of in vitro–generated red blood cells

“…It is now possible in vitro to obtain complete maturation of the erythroid line to the stage of enucleation, starting from HSCs from peripheral blood, BM, [4][5][6] umbilical cord blood 7 fetal liver, 8 from embryonic cells, [9][10][11] or adult pluripotent stem cells (induced pluripotent stem cells). [12][13][14] However, until now the clinical feasibility of this concept has not been demonstrated, whatever the origin of the cells and the experimental protocol.…”